AUTHOR=Lyu Zhongfan , Hao Yongchao , Chen Liyang , Xu Shoushen , Wang Hongjin , Li Mengyao , Ge Wenyang , Hou Bingqian , Cheng Xinxin , Li Xuefeng , Che Naixiu , Zhen Tianyue , Sun Silong , Bao Yinguang , Yang Zujun , Jia Jizeng , Kong Lingrang , Wang Hongwei 

TITLE=Wheat-Thinopyrum Substitution Lines Imprint Compensation Both From Recipients and Donors

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.837410

DOI=10.3389/fpls.2022.837410

ISSN=1664-462X

ABSTRACT=Even frequently used in wheat breeding, we still have insufficient understanding of the biology of the products via distant hybridization.  Here, a transcriptomic analysis was performed for six Triticum aestivum-Thinopyrum elongatum substitution lines in comparison with the host plants. All of the six disomic substitution lines, showed much stronger “transcriptomic-shock” occurred on alien genomes with 57.43~69.22% genes changed expression level, but little on the recipient genome (2.19~8.97%). Genome-wide suppression of alien genes along chromosomes was observed with high proportion of down-regulated genes (39.69~48.21%). Oppositely, the wheat recipient showed genome-wide compensation with more up-regulated genes, occurring on all chromosomes but not limited on the homologous groups. Moreover, strong co-up-regulation of the orthologs between wheat and Thinopyrum sub-genomes were  enriched in photosynthesis with predicted chloroplastic localization, which indicates the compensation happened not only on wheat host genomes but also on alien genomes.