AUTHOR=de Saint Germain Alexandre , Clavé Guillaume , Schouveiler Paul , Pillot Jean-Paul , Singh Abhay-Veer , Chevalier Arnaud , Daignan Fornier Suzanne , Guillory Ambre , Bonhomme Sandrine , Rameau Catherine , Boyer François-Didier 

TITLE=Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.887347

DOI=10.3389/fpls.2022.887347

ISSN=1664-462X

ABSTRACT=<p>Strigolactones (SLs) are intriguing phytohormones that not only regulate plant development and architecture but also interact with other organisms in the rhizosphere as root parasitic plants (<italic>Striga, Orobanche</italic>, and <italic>Phelipanche</italic>) and arbuscular mycorrhizal fungi. Starting with a pioneering work in 2003 for the isolation and identification of the SL receptor in parasitic weeds, fluorescence labeling of analogs has proven a major strategy to gain knowledge in SL perception and signaling. Here, we present novel chemical tools for understanding the SL perception based on the enzymatic properties of SL receptors. We designed different profluorescent SL Guillaume Clavé (GC) probes and performed structure-activity relationship studies on pea, <italic>Arabidopsis thaliana</italic>, and <italic>Physcomitrium</italic> (formerly <italic>Physcomitrella</italic>) <italic>patens</italic>. The binding of the GC probes to PsD14/RMS3, AtD14, and OsD14 proteins was tested. We demonstrated that coumarin-based profluorescent probes were highly bioactive and well-adapted to dissect the enzymatic properties of SL receptors in pea and a resorufin profluorescent probe in moss, contrary to the commercially available fluorescein profluorescent probe, Yoshimulactone Green (YLG). These probes offer novel opportunities for the studies of SL in various plants.</p>