AUTHOR=Kornpointner Christoph , Scheibelreiter Jakob , Halbwirth Heidi TITLE=Snailase: A Promising Tool for the Enzymatic Hydrolysis of Flavonoid Glycosides From Plant Extracts JOURNAL=Frontiers in Plant Science VOLUME=Volume 13 - 2022 YEAR=2022 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.889184 DOI=10.3389/fpls.2022.889184 ISSN=1664-462X ABSTRACT=Plants typically contain a broad spectrum of flavonoids in varying concentrations. As a rule, several flavonoid classes occur in parallel, and, even for a single flavonoid, divergent glycosylation patterns are frequently observed. This can be challenging in studies in which the distribution between flavonoid classes, or features that are not affected by glycosylation patterns, are addressed. Herein, we present a fast and reliable method for enzymatic hydrolysis of flavonoid glycosides from plant extracts using the commercial enzyme mix snailase, which provided the highest aglycone yields of all investigated flavonoids (aurones: leptosidin, maritimetin, sulfuretin; chalcones: butein, lanceoletin, okanin, phloretin; dihydroflavonols: dihydrokaempferol; flavanones: eriodictyol, hesperetin; flavones: acacetin, apigenin, diosmetin, luteolin; flavonols: isorhamnetin, kaempferol, myricetin, quercetin; isoflavones: biochanin A, formononetin, genistein) from methanolic extracts of nine plants (Bidens ferulifolia, Coreopsis grandiflora, Fagus sylvatica, Malus × domestica, Mentha × piperita, Petunia × hybrida, Quercus robur, Robinia pseudoacacia and Trifolium pratense) in comparison to four other enzymes (cellobiase, cellulase, β-glucosidase and pectinase), as well as to acidic hydrolysis by hydrochloric acid.