AUTHOR=Li Zibo , Sun Chunlei , Liu Xinran , Zhou Rujun 

TITLE=Molecular Identification and Analysis on Differential Pathogenicity of Cylindrocarpon Species Associated With Ginseng Root Rust Rot in Northeastern China

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.894104

DOI=10.3389/fpls.2022.894104

ISSN=1664-462X

ABSTRACT=<p><italic>Panax ginseng</italic> C. A. Meyer is one of the most important medicinal herbs in China. It is known for its high medicinal value and economic value. The ginseng root rust rot (RRR) has always been one of the important diseases troubling the ginseng industry. The yield reduction rate of RRR is ~30%. To understand why the <italic>Cylindrocarpon</italic> species bring about the ginseng RRR in Northeastern China, this study isolates 45 strains from samples collected in Liaoning, Jilin, and Heilongjiang provinces. The rDNA-internal transcribed spacer (ITS) sequence was analyzed to identify the pathogenic species. The morphological characteristics of colonies and conidia of each strain on potato dextrose agar (PDA) medium were observed, and the pathogenicity difference between different pathogenic species was analyzed by pricking method and determining the cell wall degrading enzyme activity. The BLAST alignment analysis shows that the homology of rDNA-ITS between 45 strains and <italic>Cylindrocarpon</italic> is more than 99%, among which 28 are identified as <italic>Cylindrocarpon destructans</italic>, three are identified as <italic>C. destructans</italic> var. <italic>destructans</italic>, and 14 are identified as <italic>Ilyonectria robusta</italic>. The colony diameters of all 45 isolated range from 4.21 ± 0.16a to 7.78 ± 0.25c cm after several days of incubation. Among all the species, <italic>I. robusta</italic> has the fastest growth rate, and <italic>C. destructans</italic> var. <italic>destructans</italic> has the slowest growth rate. Pathogenicity test results show that the pathogenicity of <italic>C. destructans</italic> var. <italic>destructans</italic> is the strongest, followed by <italic>C. destructans</italic>. <italic>I. robusta</italic> has relatively weak pathogenicity.</p>