AUTHOR=Wu Junyan , Xu Xin-Dong , Liu Lijun , Ma Li , Pu Yuanyuan , Wang Wangtian , Hua Xue-Yang , Song Jia-Ming , Liu Kede , Lu Guangyuan , Fang Yan , Li Xuecai , Sun Wancang 

TITLE=A Chromosome Level Genome Assembly of a Winter Turnip Rape (Brassica rapa L.) to Explore the Genetic Basis of Cold Tolerance

JOURNAL=Frontiers in Plant Science

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.936958

DOI=10.3389/fpls.2022.936958

ISSN=1664-462X

ABSTRACT=<p>Winter rapeseed (<italic>Brassica rapa L</italic>.) is an important overwintering oilseed crop that is widely planted in northwest China and suffers chronic low temperatures in winter. So the cold stress becomes one of the major constraints that limit its production. The currently existing genomes limit the understanding of the cold-tolerant genetic basis of rapeseed. Here we assembled a high-quality long-read genome of <italic>B. rapa</italic> “Longyou-7” cultivar, which has a cold-tolerant phenotype, and constructed a graph-based pan-genome to detect the structural variations within homologs of currently reported cold-tolerant related genes in the “Longyou-7” genome, which provides an additional elucidation of the cold-tolerant genetic basis of “Longyou-7” cultivar and promotes the development of cold-tolerant breeding in <italic>B. rapa</italic>.</p>