AUTHOR=Chen Qianqian , Li Jifu , Liu Guoxuan , Lu Xing , Chen Kang , Tian Jiang , Liang Cuiyue 

TITLE=A Berberine Bridge Enzyme-Like Protein, GmBBE-like43, Confers Soybean's Coordinated Adaptation to Aluminum Toxicity and Phosphorus Deficiency

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 13 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.947986

DOI=10.3389/fpls.2022.947986

ISSN=1664-462X

ABSTRACT=<p>Phosphorus (P) deficiency and aluminum (Al) toxicity often coexist and are two major limiting factors for crop production in acid soils. The purpose of this study was to characterize the function of <italic>GmBBE-like43</italic>, a berberine bridge enzyme-like protein-encoding gene, in soybean (<italic>Glycine max</italic>) adaptation to Al and low P stresses. Present quantitative real-time PCR (qRT-PCR) assays confirmed the phosphate (Pi)-starvation enhanced and Al-stress up-regulated expression pattern of <italic>GmBBE-like43</italic> in soybean roots. Meanwhile, the expression of a <italic>GmBBE-like43-GFP</italic> chimera in both common bean hairy roots and tobacco leaves demonstrated its cell wall localization. Moreover, both transgenic Arabidopsis and soybean hairy roots revealed the function of <italic>GmBBE-like43</italic> in promoting root growth under both Al and low P stresses. <italic>GmBBE-like43</italic>-overexpression also resulted in more H<sub>2</sub>O<sub>2</sub> production on transgenic soybean hairy root surface with oligogalacturonides (OGs) application and antagonized the effects of Al on the expression of two <italic>SAUR-like</italic> genes. Taken together, our results suggest that <italic>GmBBE-like43</italic> might be involved in the soybean's coordinated adaptation to Al toxicity and Pi starvation through modulation of OGs-oxidation in the cell wall.</p>