AUTHOR=Huang Lanlan , Li Yongmei , Yuan Jing , Wan Shanping , Colinas Carlos , He Xinhua , Shi Xiaofei , Wang Yanliang , Yu Fuqiang 

TITLE=Tuber indicum and T. lijiangense colonization differentially regulates plant physiological responses and mycorrhizosphere bacterial community of Castanopsis rockii seedlings

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1134446

DOI=10.3389/fpls.2023.1134446

ISSN=1664-462X

ABSTRACT=Black truffles and white truffles are widely studied around the world, but their effects on plant growth and physiological responses, as well as ectomycorrhizal-sphere bacterial communities of the same host remains unclear. Here, a greenhouse-based mycorrhization of Castanopsis rockii by Tuber indicum (black truffle) and T. lijiangense (white truffle) respectively was conducted and their effects on host growth, physiological responses and ectomycorrhizal-sphere bacterial communities were compared. The results show that both Tuber species colonization could significantly increase leaf photosynthetic rate, leaf P concentration and rhizosphere acid phosphatase activity, as well as richness of rhizosphere bacterial communities of C. rockii seedlings. However, T. indicum colonization significantly decreased tartrate content and bacterial acid phosphatase and phoC gene abundance in the mycorrhizosphere, and peroxidase (POD) activity of ectomycorrhizal (ECM) tips but increased mycorrhizosphere pH and ECM superoxide dismutases (SOD) activity, compared to T. lijiangense colonization. Moreover, principal coordinate analysis and β-diversity results show significant difference in mycorrhizosphere bacterial community structures between T. indicum and T. lijiangese colonized C. rockii seedlings. Furthermore, the relative abundance of Agromyces cerinus have significant positive correlation with mycorrhizosphere acid phosphatase activity and leaf P concentration, suggesting that it might play an important role in P mobilization and acquisition. Overall, these results suggest that T. indicum (black truffle) and T. lijiangense (white truffle) differently regulate their host plant’s physiological responses and rhizosphere bacterial communities.