AUTHOR=Ijaz Siddra , Ul Haq Imran , Razzaq Hafiza Arooj , Nasir Bukhtawer , Ali Hayssam M. , Kaur Sukhwinder 

TITLE=In silico structural-functional characterization of three differentially expressed resistance gene analogs identified in Dalbergia sissoo against dieback disease reveals their role in immune response regulation

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1134806

DOI=10.3389/fpls.2023.1134806

ISSN=1664-462X

ABSTRACT=Plant immunity implicates enemy recognition, signal transduction, and defensive response against pathogens. We experimented to identify the genes that contribute resistance against  dieback disease to Dalbergia sissoo, an economically important timber tree. In this study, we investigated the role of three differentially expressed genes identified in the dieback-induced transcriptome in Dalbergia sissoo. The transcriptome was probed using DOP-rtPCR analysis. The identified RGAs were in silico characterized as the contributors of disease resistance that switch on under dieback stress. Their predicted fingerprints revealed involvement in stress response. The Ds-DbRCaG-02-Rga.a, Ds-DbRCaG-04-Rga.b, and Ds-DbRCaG-06-Rga.c, showed structural homology with Transthyretin-52 domain, EAL associated YkuI_C domain, and Src homology-3 domain respectively, which are the attributes of signaling proteins possessing role in regulating immune responses in plants. Based on in-silico structural and functional characterization, their role was predicted in immune response regulation in D. sissoo.