AUTHOR=Guo Susu , Ji Yanhai , Zheng Yanyan , Watkins Christopher B. , Ma Lili , Wang Qing , Liang Hao , Bai Chunmei , Fu Anzhen , Li Ling , Meng Demei , Liu Mingchi , Zuo Jinhua 

TITLE=Transcriptomic, metabolomic, and ATAC-seq analysis reveal the regulatory mechanism of senescence of post-harvest tomato fruit

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1142913

DOI=10.3389/fpls.2023.1142913

ISSN=1664-462X

ABSTRACT=Some physiological changes occurred during fruit storage, which involves the regulation of genes, metabolisms and transcription factors. Here, we compared two different varieties of tomato fruit (Solanum lycopersicum), namely ‘JF308’ (a normal tomato cultivar) and ‘YS006’ (a storable tomato cultivar), to determine difference of accumulated metabolites, gene expression, and accessible chromatin regions by metabolome, transcriptome, and ATAC-seq. A total of 1006 metabolites were identified in two cultivars. Sugars, alcohols, and flavonoids were more abundant in ‘YS006’ than ‘JF308’ during day 7, day14, and day 21 storage time, respectively. Differentially expressed genes, which involved in starch and sucrose biosynthesis were observed higher in ‘YS006’. ‘YS006’ had lower expression levels of CesA(cellulose synthase), PL (pectate lyase), EXPA(expansin) and XTH(xyloglucan endoglutransglucosylase/hydrolase) than ‘JF308’. phenylpropanoid pathway, carbohydrate metabolism and cell wall metabolism play important roles in prolonging the shelf-life of tomato fruit. ATAC-seq revealed that the TCP 2, 3, 4, 5 and 24 belong to basic helix-loop-helix factors class, are the most significant up-regulated transcription factors in J21 vs. Y21 (‘JF308’ was compared with ‘YS006’ on the day 21 of storage). The information on the molecular regulatory mechanisms and metabolic pathways of post-harvest quality changes in tomato fruit provides a theoretical foundation for slowing post-harvest fruit quality decay and loss, and has theoretical importance and application value in breeding for longer shelf life cultivars.