AUTHOR=Magembe Eric Maina , Li Hui , Taheri Ali , Zhou Suping , Ghislain Marc 

TITLE=Identification of T-DNA structure and insertion site in transgenic crops using targeted capture sequencing

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1156665

DOI=10.3389/fpls.2023.1156665

ISSN=1664-462X

ABSTRACT=Genetically engineered (GE) crops must go through a pre-market safety assessment to ensure they are safe for humans, animals, and the environment. The molecular characterization of the T-DNA insertion is a critical step for selecting a transgenic event bearing a T-DNA with all transgenes intact, without multiple full or partial copies of it, and with an insertion into a known region of the crop genome. This has long been a tedious process using multiple PCR techniques and Sanger sequencing methods, which limits the number of transgenic events that can be assessed.