AUTHOR=Choudhary Nisha , Tandi Dipty , Verma Rakesh Kumar , Yadav Virendra Kumar , Dhingra Naveen , Ghosh Tathagata , Choudhary Mahima , Gaur Rajarshi K. , Abdellatif Magda H. , Gacem Amel , Eltayeb Lienda Bashier , Alqahtani Mohammed S. , Yadav Krishna Kumar , Jeon Byong-Hun TITLE=A comprehensive appraisal of mechanism of anti-CRISPR proteins: an advanced genome editor to amend the CRISPR gene editing JOURNAL=Frontiers in Plant Science VOLUME=Volume 14 - 2023 YEAR=2023 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1164461 DOI=10.3389/fpls.2023.1164461 ISSN=1664-462X ABSTRACT=

The development of precise and controlled CRISPR-Cas tools has been made possible by the discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPRs (Acrs). The Acr protein has the ability to control off-targeted mutations and impede Cas protein–editing operations. Acr can help with selective breeding, which could help plants and animals improve their valuable features. In this review, the Acr protein–based inhibitory mechanisms that have been adopted by several Acrs, such as (a) the interruption of CRISPR-Cas complex assembly, (b) interference with target DNA binding, (c) blocking of target DNA/RNA cleavage, and (d) enzymatic modification or degradation of signalling molecules, were discussed. In addition, this review emphasizes the applications of Acr proteins in the plant research.