AUTHOR=Choudhary Nisha , Tandi Dipty , Verma Rakesh Kumar , Yadav Virendra Kumar , Dhingra Naveen , Ghosh Tathagata , Choudhary Mahima , Gaur Rajarshi K. , Abdellatif Magda H. , Gacem Amel , Eltayeb Lienda Bashier , Alqahtani Mohammed S. , Yadav Krishna Kumar , Jeon Byong-Hun 

TITLE=A comprehensive appraisal of mechanism of anti-CRISPR proteins: an advanced genome editor to amend the CRISPR gene editing

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1164461

DOI=10.3389/fpls.2023.1164461

ISSN=1664-462X

ABSTRACT=The development of extremely controlled and precise CRISPR-Cas tools has been made possible by the discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPRs (Acrs). The Anti-CRISPR protein has the ability to control off-targeted mutations as well as impede Cas protein editing operations. Anti-CRISPR can help with selective breeding, which could help plants and animals improve their valuable features. In this review, the anti-CRISPR protein-based inhibitory mechanisms that have been adopted by several Acrs, such as (a) the interruption of CRISPR-Cas complex assembly, (b) interference with target DNA binding, (c) blocking of target DNA/RNA cleavage, and (d) enzymatic modification or degradation of signalling molecules, were discussed. In addition, this review emphasises the applications of anti-CRISPR proteins in therapeutics and plant research.