AUTHOR=Li Jitong , Liu Hao , Lv Xinyi , Wang Wenjuan , Liang Xinyan , Chen Lin , Wang Yiping , Liu Jinglan 

TITLE=A key ABA biosynthetic gene OsNCED3 is a positive regulator in resistance to Nilaparvata lugens in Oryza sativa

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 15 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1359315

DOI=10.3389/fpls.2024.1359315

ISSN=1664-462X

ABSTRACT=<p>The gene encoding 9-cis-epoxycarotenoid dioxygenase 3 (<italic>NCED3</italic>) functions in abscisic acid (ABA) biosynthesis, plant growth and development, and tolerance to adverse temperatures, drought and saline conditions. In this study, three rice lines were used to explore the function of <italic>OsNCED3</italic>, these included an OsNCED3-overexpressing line (<italic>OsNCED3-OE</italic>), a knockdown line (<italic>osnced3-RNAi</italic>) and wild-type rice (WT). These rice lines were infested with the brown plant hopper (BPH; <italic>Nilaparvata lugens</italic>) and examined for physiological and biochemical changes, hormone content, and defense gene expression. The results showed that <italic>OsNCED3</italic> activated rice defense mechanisms, which led to an increased defense enzyme activity of superoxide dismutase, peroxidase, and polyphenol oxidase. The overexpression of <italic>OsNCED3</italic> decreased the number of planthoppers and reduced oviposition and BPH hatching rates. Furthermore, the overexpression of <italic>OsNCED3</italic> increased the concentrations of jasmonic acid, jasmonyl-isoleucine and ABA relative to WT rice and the <italic>osnced3-RNAi</italic> line. These results indicate that <italic>OsNCED3</italic> improved the stress tolerance in rice and support a role for both jasmonates and ABA as defense compounds in the rice-BPH interaction.</p>