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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Biotechnology
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1400301
This article is part of the Research Topic Molecular and Physiological Mechanisms Driving Phytoremediation View all articles

Comprehensive analysis of B3 family genes in pearl millet (Pennisetum glaucum) and the negative regulator role of PgRAV-04 in drought tolerance

Provisionally accepted
YinHua Wang YinHua Wang 1Xing Ye Xing Ye 1*BiYao Zhao BiYao Zhao 1*WengJing Wang WengJing Wang 1ZhengFeng Zhou ZhengFeng Zhou 1*XiangQian Zhang XiangQian Zhang 2*Juan Du Juan Du 3JianLing Song JianLing Song 4XiaoLing Huang XiaoLing Huang 1*Kunxi Ouyang Kunxi Ouyang 1Tianxiu Zhong Tianxiu Zhong 1*FeiXiong Liao FeiXiong Liao 1*
  • 1 South China Agricultural University, Guangzhou, China
  • 2 Foshan University, Foshan, Guangdong Province, China
  • 3 Oklahoma State University, Stillwater, Oklahoma, United States
  • 4 Xingyi Normal University for Nationalities, Xingyi, Guizhou, China

The final, formatted version of the article will be published soon.

    Members of the plant-specific B3 transcription factor superfamily play crucial roles in various plant growth and developmental processes. Despite numerous valuable studies on B3 genes in other species, little is known about the B3 superfamily in pearl millet. Through comparative genomic analysis, we identified 70 B3 proteins in pearl millet and categorized them into four subfamilies based on phylogenetic affiliations: ARF, RAV, LAV, and REM. We also mapped the chromosomal locations of these proteins and analyzed their gene structures, conserved motifs, and gene duplication events, providing new insights into their potential functional interactions.Using transcriptomic sequencing and real-time quantitative PCR, we determined that most PgB3 genes exhibit upregulated expression under drought and high-temperature stresses, indicating their involvement in stress response regulation. To delve deeper into the abiotic stress roles of the B3 family, we focused on a specific gene within the RAV subfamily, PgRAV-04, cloning it and overexpressing it in tobacco. PgRAV-04 overexpression led to increased drought sensitivity in the transgenic plants due to decreased proline levels and peroxidase activity. This study not only adds to the existing body of knowledge on the B3 family's characteristics but also advances our functional understanding of the PgB3 genes in pearl millet, reinforcing the significance of these factors in stress adaptation mechanisms.

    Keywords: B3 superfamily, drought tolerance, Gene Expression, pearl millet, PgRAV-

    Received: 13 Mar 2024; Accepted: 08 Jul 2024.

    Copyright: © 2024 Wang, Ye, Zhao, Wang, Zhou, Zhang, Du, Song, Huang, Ouyang, Zhong and Liao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Xing Ye, South China Agricultural University, Guangzhou, China
    BiYao Zhao, South China Agricultural University, Guangzhou, China
    ZhengFeng Zhou, South China Agricultural University, Guangzhou, China
    XiangQian Zhang, Foshan University, Foshan, 528000, Guangdong Province, China
    XiaoLing Huang, South China Agricultural University, Guangzhou, China
    Tianxiu Zhong, South China Agricultural University, Guangzhou, China
    FeiXiong Liao, South China Agricultural University, Guangzhou, China

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