The final, formatted version of the article will be published soon.
ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Pathogen Interactions
Volume 15 - 2024 |
doi: 10.3389/fpls.2024.1411837
This article is part of the Research Topic Digging Deeper: Understanding Root-Pathogen Interactions View all 5 articles
Plant-induced bacterial gene silencing: a novel control method for bacterial wilt disease
Provisionally accepted- Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea
Ralstonia pseudosolanacearum, a notorious phytopathogen, is responsible for causing bacterial wilt, leading to significant economic losses globally in many crops within the Solanaceae family. Despite various cultural and chemical control strategies, managing bacterial wilt remains a substantial challenge. This study demonstrates, for the first time, the effective use of plant-induced bacterial gene silencing against R. pseudosolanacearum, facilitated by Tobacco rattle virus-mediated gene silencing, to control bacterial wilt symptoms in Nicotiana benthamiana. The methodology described in this study could be utilized to identify novel phytobacterial virulence factors through both forward and reverse genetic approaches. To validate plant-induced gene silencing, small RNA fractions extracted from plant exudates were employed to silence bacterial gene expression, as indicated by the reduction in the expression of GFP and virulence genes in R. pseudosolanacearum.Furthermore, treatment of human and plant pathogenic Gram-negative and Gram-positive bacteria with plant-generated small RNAs resulted in the silencing of target genes within 48 hours. Taken together, the results suggest that this technology could be applied under field conditions, offering precise, gene-based control of target bacterial pathogens while preserving the indigenous microbiota.
Keywords: host-induced gene silencing, plant-induced bacterial gene silencing, Ralstonia pseudosolanacearum, Agrobacterium tumefaciens, Tobacco rattle virus
Received: 03 Apr 2024; Accepted: 16 Jul 2024.
Copyright: © 2024 Jang, Kim, Lee and Ryu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Choong-Min Ryu, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 306-809, Republic of Korea
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Doyeon Kim