AUTHOR=Li Linbao , Huang Guiyun , Wu Jinhua , Yu Yunchao , Zhang Guangxin , Su Yang , Wang Xiongying , Chen Huiyuan , Wang Yeqing , Wu Di 

TITLE=Combine photosynthetic characteristics and leaf hyperspectral reflectance for early detection of water stress

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2025.1520304

DOI=10.3389/fpls.2025.1520304

ISSN=1664-462X

ABSTRACT=Advanced techniques capable of early and non-destructive detection of the impacts of water stress on trees and estimation of the underlying photosynthetic capacities on larger scale are necessary to meet the challenges of limiting plant growth and ecological protection caused by drought. We tested influence of continuous water stress on photosynthetic traits including Leaf Chlorophyll content (LCC) and Chlorophyll Fluorescence (ChlF) and combined hyperspectral reflectance as a high-throughput approach for early and non-destructive assessment of LCC and ChlF traits in Rhamnus leptophylla trees. LCC and ChlF parameters (NPQ, Fv’/Fm’, ETR, ETRmax, Fm’, qL, qP, Y(II) were measured alongside leaf hyperspectral reflectance from Rhamnus leptophylla suffering from constant drought during water stress. Water stress caused NPQ, Fv’/Fm’, ETRmax, Fm’, qL, qP, Y(II) and ETR continuous decline throughout the entire drought period. ChlF was more sensitive to drought monitoring than LCC. The original reflectance spectra and hyperspectral vegetation indices (SVIs) showed a strong correlation with LCC and ChlF. Reflectance in 540-560nm and 750-1100nm and selected SVI such as Simple Ratio (SR)752/690 can track drought responses effectively before leaves showed drought symptoms. Multivariate Linear Regression (MLR) and three machine learning algorithms, namely Random Forest (RF), Support Vector Machine (SVM), and K-Nearest Neighbor (KNN) were employed to develop models for estimating LCC and ChlF parameters. RF provided the best estimation accuracy for LCC compared to MLR, KNN and SVM, achieving an R2 value of 0.895 for all LCC samples. The canopy layer significantly influenced the estimation accuracy of LCC, with the middle layer yielding the highest R2 value. RF also demonstrated superior performance compared to MLR, KNN and SVM for estimating NPQ, Fv’/Fm’, ETRmax, Fm’, qL, qP, Y(II) and ETR, achieving R2 value of 0.854 for NPQ, 0.610 for Fv’/Fm’, 0.878 for ETRmax, 0.676 for Fm’, 0.604 for qL, 0.731 for qP, 0.879 for Y(II), and 0.740 for ETR. Our results indicate that photosynthetic traits combined hyperspectral reflectance can monitor the effect of drought on trees effectively with significant potential for monitoring drought over large areas.