AUTHOR=Zhao Peiyan , Zhou Houfa , Liao Xiaolin , Zhao Leifeng , Zheng Yuanxian , Xiong Tiane , Zhang Gaorun , Jiang Sirong , Wang Jiming , He Yuansheng , Li Jiangtao , Zhu Jieying , Zhang Yongjun , Li Yanrun , Nian Fuzhao , Liu Di 

TITLE=The regulation of tobacco growth under preceding crop planting: insights from soil quality, microbial communities, and metabolic profiling

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2025.1530324

DOI=10.3389/fpls.2025.1530324

ISSN=1664-462X

ABSTRACT=IntroductionSpecific microorganisms and metabolites in soil play key roles in regulating organismal behavior. Currently, the effects of different preceding crops on the rhizosphere soil quality of flue-cured tobacco remain unclear.MethodsFour treatments were compared in the study: fallow + tobacco (CK), maize + tobacco (T1), rapeseed + tobacco (T2), and wheat + tobacco (T3).Results and discussionResults showed that preceding crops significantly enhanced soil nutrient levels and improved tobacco growth by altering rhizosphere metabolites and microbial community structure. Previous cultivation of maize and rapeseed significantly promoted tobacco growth, rapeseed and wheat cultivation enhanced the diversity of soil bacterial communities, and notably decreased the abundance of urea-degrading bacteria. In contrast, the preceding crop of maize reduced plant pathogenic fungi and promoted positive microbial interactions. Metabolomics analysis showed that different preceding crops altered lipids, organic acids, flavonoids, alkaloids, and terpenoids, enhancing secondary metabolite synthesis pathways in soil. Preceding crops regulated rhizosphere metabolites which potentially participated in soil carbon and nitrogen cycling, balancing soil nutrients, and improving tobacco yield. Overall, the three preceding crops altered the composition and function of metabolites and microbial community structures in rhizosphere soil, thereby increased soil nutrient concentration. Both maize and rapeseed cultivation significantly boosted tobacco growth and biomass. These findings offer new insights into the potential interactions between rhizosphere metabolites and microbial communities and strategies of comprehensively regulating tobacco growth.