AUTHOR=Wang Jingang , Wang Haijiang , Lv Xin , Cui Jing , Shi Xiaoyan , Song Jianghui , Li Weidi , Zhang Wenxu 

TITLE=Estimating photosynthetic characteristics of forage rape by fusing the sensitive spectral bands to combined stresses of nitrogen and salt

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2025.1547832

DOI=10.3389/fpls.2025.1547832

ISSN=1664-462X

ABSTRACT=Leaf gas exchange and chlorophyll fluorescence parameters (PGE-CFPs), which respond significantly and quickly to environmental stresses, have been used to assess the early responses of crop physiology to stresses. Most spectral estimations only focus on crop photosynthetic characteristics under a single environmental stress. Thus, the methods proposed previously are not suitable for the estimations under combined stresses (i.e., nitrogen and salt). In this research, the leaf spectral features of forage rape (Brassica napus L.) under nitrogen stress (NSpe) and salt stress (SSpe) were fused to increase the accuracy of the spectral estimation of photosynthetic characteristics of forage rape under combined stresses in arid region of Xinjiang, China. The results showed that PGE-CFPs’ spectral features were extracted with SPA (successive projections algorithm) after preprocessing. Among the SSpe- and NSpe-based models, the RF (random forest) models had higher estimation accuracy than the PLSR (partial least squares regression) and BPNN (backpropagation neural network) models. Specifically, the RF models had a PGE-CFPs estimation accuracy of 0.597–0.712, 0.640–0.715, and 0.377–0.461 under nitrogen stress (NS), salt stress (SS), and NS*SS, respectively. After fusing NSpe and SSpe, the accuracy in estimating PGE-CFPs of forage rape under NS, SS, and NS*SS were 0.729–0.755, 0.667–0.768, and 0.621–0.689, respectively. Then, the constructed models were further validated using field data, and the accuracy obtained was in the range of 0.585–0.711. Therefore, the feature fusion modeling method proposed has strong transferability and applicability. This research will offer a technical reference for crop photosynthesis monitoring at the early stage of environmental stresses.