AUTHOR=Wang Mingxia , Zhao Ben , Niu Xiaoli , Chu Wanqiang , Lv Guijun 

TITLE=Exploring the effect of plant nitrogen concentration on the nitrogen nutrition index of winter wheat under controlled irrigation conditions

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2025.1609847

DOI=10.3389/fpls.2025.1609847

ISSN=1664-462X

ABSTRACT=IntroductionThe nitrogen nutrition index (NNI) of winter wheat decreased under water deficit conditions, primarily due to an increase in the critical nitrogen concentration (%Nc) associated with a reduction in shoot biomass (SB). However, the effect of plant nitrogen concentration (PNC) on NNI under water deficit conditions remains unclear. This study aimed to: (1) determine whether significant differences in PNC and leaf nitrogen concentration (LNC) of winter wheat exist among different water treatments under controlled conditions; (2) analyze the reasons for changes in PNC and LNC under water deficit conditions; and (3) assess the stability of relationships between PNC and LNC, as well as between plant nitrogen accumulation (NAp) and leaf area index (LAI), across different water treatments.MethodsTo address the above mentioned objectives, a series of rainout shelter experiments were conducted during the winter wheat growing seasons from 2018 to 2021.Results and discussionThe results indicated that water deficit treatments limited PNC and LNC values at specific growth stages of winter wheat under controlled conditions. However, such severe water deficits are unlikely to occur in typical field conditions; thus, PNC was not identified as the primary factor affecting NNI in field environments experiencing water deficit. Component analysis clarified the causes behind the decline in PNC and LNC. The decline in specific leaf area (SLA) and leaf biomass fraction (LBF) contributed to the decrease in PNC, with SLA accounting for more variation than LBF. Similarly, declines in both SLA and specific leaf nitrogen (SLN) led to reduced LNC, with SLN explaining more variation in LNC than SLA across different water treatments. LNC was jointly controlled by both PNC and the ratio of SLN to LBF. Furthermore, water deficit did not alter the proportional linear relationship between NAp and LAI, suggesting that the impact of water deficit on PNC and LNC is limited, which helps a better understanding of the factors contributing to the declination of NNI.