AUTHOR=Wu Xuemei , Zhong Liwen , Ding Rong , Wang Chenghui , Chen Hongchuan , Zhong Shihong , Gu Rui 

TITLE=Non-destructive estimation of SPAD and biomass in Lamiophlomis rotata using hyperspectral imaging and deep learning with DRSA-CARS feature selection

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2025.1640779

DOI=10.3389/fpls.2025.1640779

ISSN=1664-462X

ABSTRACT=IntroductionMonitoring the growth status and aboveground biomass of wild and cultivated medicinal herbs remains a persistent challenge in precision agriculture.MethodsIn this study, we developed machine learning and deep learning models to estimate SPAD values and biomass of Lamiophlomis rotata (Benth.). The models used hyperspectral data and time-series phenotypic traits from 508 samples collected across different altitudes. Regions of interest (ROIs) were manually defined from plant contours. The corresponding mean spectral profiles were then preprocessed. To improve feature selection, we proposed a Dynamic Reptile Search Algorithm-enhanced CARS (DRSA-CARS) method. This method integrates a dynamic behavioral strategy into the CARS framework to identify informative spectral bands. Vegetation indices (VIs) and gray-level co-occurrence matrix (GLCM)-based texture parameters were extracted and combined with spectral features to construct the PLSR, SVR, FNN, and CNN models.ResultsCompared to CARS, the DRSA-CARS method reduced feature dimensionality by up to 75.7% for SPAD and 29.2% for biomass, while improving prediction accuracy (R²) by 24.4% and 34.7%, respectively. Among all models, the FNN achieved the highest performance, with R² values of 0.7732 (training) and 0.7502 (testing) for SPAD and 0.8260 and 0.7933 for biomass. Feature fusion further improved predictive accuracy by 11% for SPAD and 30% for biomass compared to models based on individual feature types.DiscussionThese results demonstrate that coupling DRSA-CARS-based feature selection with deep learning provides a robust, non-destructive approach for evaluating plant growth status. This framework highlights the potential of hyperspectral imaging as a rapid, reliable, non-invasive tool for precision cultivation of medicinal herbs.