AUTHOR=Yang Weiyue , Li Jinshan , Feng Yayang , Li Xuemin , Zheng Rui , Sun Xiulu 

TITLE=Deep learning-based approach for phenotypic trait extraction and computation of tomato under varying water stress

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2025.1660593

DOI=10.3389/fpls.2025.1660593

ISSN=1664-462X

ABSTRACT=IntroductionWith the advancement of imaging technologies, the efficiency of acquiring plant phenotypic information has significantly improved. The integration of deep learning has further enhanced the automatic recognition of plant structures and the accuracy of phenotypic parameter extraction. To enable efficient monitoring of tomato water stress, this study developed a deep learning-based framework for phenotypic trait extraction and parameter computation, applied to tomato images collected under varying water stress conditions.MethodsBased on the You Only Look Once version 11 nano (YOLOv11n) object detection model, adaptive kernel convolution (AKConv) was integrated into the backbone’s C3 module with kernel size 2 convolution (C3k2), and a recalibration feature pyramid detection head based on the P2 layer was designed.Results and discussionResults showed that the improved model achieved a 4.1% increase in recall, a 2.7% increase in mAP50, and a 5.4% increase in mAP50–95 for tomato phenotype recognition. Using the bounding box information extracted by the model, key phenotype parameters were further calculated through geometric analysis. The average relative error for plant height was 6.9%, and the error in petiole count was 10.12%, indicating good applicability and accuracy for non-destructive crop phenotype analysis. Based on these extracted traits, multiple sets of weighted combinations were constructed as input features for classification. Seven classification algorithms—Logistic Regression, Support Vector Machine, Random Forest, Decision Tree, K-Nearest Neighbors, Naive Bayes, and Gradient Boosting—were used to differentiate tomato plants under different water stress conditions. The results showed that Random Forest consistently performed the best across all combinations, with the highest classification accuracy reaching 98%. This integrated approach provides a novel approach and technical support for the early identification of water stress and the advancement of precision irrigation.