AUTHOR=Zhao Yan , Fang Jiaqi , Gislum René , Zhao Baowen , Zhong Zhiming , Lei Yingxia , Yan Donghai , He Ruzhi , Chen Youjun , Zhou Qingping , Wang Hui 

TITLE=Genetic diversity analysis of phenotypic and agronomic traits in oat germplasm resources

JOURNAL=Frontiers in Plant Science

VOLUME=Volume 16 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2025.1670684

DOI=10.3389/fpls.2025.1670684

ISSN=1664-462X

ABSTRACT=The evaluation of genetic diversity in germplasm resources is fundamental to crop breeding. A total of 183 oat germplasm resources were evaluated through field trials at Xinjin District and Shandan County, located in southern and northern China, respectively. Phenotypic and agronomic traits were assessed, including six qualitative and sixteen quantitative characteristics. Results revealed significant variation in  panicle attitude and grain color, based on the statistical analysis using SPSS. Among the sixteen quantitative traits, coefficient of variation ranged from 4.92% to 48.02% with the second internode thickness exhibiting the highest genetic diversity index. Correlation analysis of sixteen quantitative traits was performed using R Studio, and the results indicated significant positive relationships between grain weight and several ear characteristics, including spikelet number, ear length, layer numbers, and grain numbers per ear. Principal component analysis categorized the sixteen quantitative phenotypic traits into five independent factors. The structural equation modeling using SPSS-AMOS indicated that ear characteristics showed strong direct contributions to grain weight, establishing it as a key indicator for future breeding efforts. The multiple correspondence analysis by R Studio suggested that a total of nineteen oat germplasm resources showed the grain and biomass production potential across both experimental regions.