AUTHOR=Elnagar Asmaa , El-Dawy Khalifa , El-Belbasi Hussein I. , Rehan Ibrahim F. , Embark Hamdy , Al-Amgad Zeinab , Shanab Obeid , Mickdam Elsayed , Batiha Gaber E. , Alamery Salman , Fouad Samer S. , Cavalu Simona , Youssef Mohammed 

TITLE=Ameliorative Effect of Oxytocin on FBN1 and PEPCK Gene Expression, and Behavioral Patterns in Rats' Obesity-Induced Diabetes

JOURNAL=Frontiers in Public Health

VOLUME=Volume 10 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/public-health/articles/10.3389/fpubh.2022.777129

DOI=10.3389/fpubh.2022.777129

ISSN=2296-2565

ABSTRACT=Amelioration of hyperinsulinemia and insulin resistance associated with obesity is a cardinal target for therapeutics. Therefore, we investigated the relation of Fibrilln-1 (FBN1) mRNA expression and hepatic phosphoenolpyruvate caboxykinase (PEPCK) enzyme to the ameliorative impact of oxytocin on obesity-induced diabetes, suggesting glycogenolysis markers in diabetic models. Four groups of forty male Wistar rats were formed (n=10): a control one fed basal diet and intraperitoneal injected of saline; an oxytocin-injected group; a diet-induced obese one fed high-fat/high-sugar diet and injected with saline; a diet-induced obese group injected with oxytocin. Depending on blood glucose levels, obese groups were further sub-grouped into prediabetic, and diabetic rats, with 5 rats each, at the 9th and the16th week of the feeding period, respectively. FBN1 expression and PEPCK activity were determined using the qPCR technique and some biochemical parameters (glycemic, lipid profile, kidney and liver functions) were determined using kits. Obese groups showed an elevation of brain FBN1 expression, high serum lipid profile, high glucose level, and a deleterious impact on liver and kidney functions. Obese groups showed stimulator effect of PEPCK enzyme and time-dependent pathological changes in renal and hepatic tissues. The motor activities wee negatively correlated with FBN1 gene expression in prediabetic rats and diabetic ones. In addition to our previous reviewing of the crucial role of asprosin, here we showed that oxytocin could ameliorate obesity-induced diabetes and decrease FBN1 gene expression centrally to block appetite. Also, oxytocin caused decreasing in PEPCK enzyme activity as well as glycogenolysis in the liver. Therefore, oxytocin has a potential effect on FBN1 expression and PEPCK enzyme activity in the obesity-induced diabetic-rat model.