AUTHOR=Lertpanprom Muntinee , Silsirivanit Atit , Tippayawat Patcharaporn , Proungvitaya Tanakorn , Roytrakul Sittiruk , Proungvitaya Siriporn 

TITLE=High expression of protein tyrosine phosphatase receptor S (PTPRS) is an independent prognostic marker for cholangiocarcinoma

JOURNAL=Frontiers in Public Health

VOLUME=Volume 10 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/public-health/articles/10.3389/fpubh.2022.835914

DOI=10.3389/fpubh.2022.835914

ISSN=2296-2565

ABSTRACT=Cholangiocarcinoma (CCA) is an aggressive tumor of the bile duct with high rate of mortality. Lymph node metastasis is an important factor facilitating the progression of CCA. A reliable biomarker for diagnosis, progression status or prognosis of CCA is still lacking. To identify a novel and reliable biomarker for diagnosis/prognosis of CCA, liquid chromatography-mass spectrometry and tandem mass spectrometry (LC-MS/MS) in combination with bioinformatic analysis was applied for the representative serum samples of CCA patients. The proteome results showed that protein tyrosine phosphatase receptor S (PTPRS) had the highest potential candidate. Then, a dot blot assay was used to measure the level of serum PTPRS in CCA patients (n=80), benign biliary disease patients (BBD; n=39) and healthy controls (HC; n=55). PTPRS level of CCA sera (14.38 ± 9.42 ng/mL) was significantly higher than that of BBD (10.70 ± 5.05 ng/mL) or HC (6.00 ± 3.73 ng/mL) (P< 0.0001). PTPRS was associated with serum albumin (P=0.028), lymph node metastasis (P=0.038), and the survival time of patients (P=0.011). Using a log-rank test, higher serum PTPRS level was significantly (P=0.031) correlated with longer overall survival time of CCA patients, and PTPRS was an independent prognostic marker for CCA superior to carbohydrate antigen 19-9 (CA19-9), carcinoembryonic antigen (CEA) or alkaline phosphatase (ALP). High expression of PTPRS could be a good independent prognostic marker for CCA.