AUTHOR=Song Zexuan , Liu Zhi , Ma Aijing , Liu Chunfa , He Wencong , Zeng Xiangjie , Wang Yiting , He Ping , Liu Dongxin , Zhao Bing , Xia Hui , Wang Shengfen , Zhao Yanlin 

TITLE=High diversity of clinical Mycobacterium intracellulare in China revealed by whole genome sequencing

JOURNAL=Frontiers in Public Health

VOLUME=Volume 10 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/public-health/articles/10.3389/fpubh.2022.989587

DOI=10.3389/fpubh.2022.989587

ISSN=2296-2565

ABSTRACT=Mycobacterium intracellulare is the most common cause of nontuberculous mycobacterial lung disease, with a rapidly growing prevalence worldwide. Interesting, we found that 89 (76.07%) belong to Mycobacterium. indicus prani compared the whole genome sequence of 117 clinical isolates that were previous identified with M. intracellulare. The phylogenetic analysis showed M. indicus pranii and M. intracellulare isolates are diverse and not cluster. M. indicus pranii may be considered as causative agent of chronic lung disease. Furthermore, our data demonstrated that clarithromycin, amikacin and rifabutin showed strong antimicrobial activity against both M. intracellulare and M. indicus pranii in vitro. However, there is no clear correction between the rrs, rrl, DNA gyrase genes (gyrA and gyrB) and the aminoglycoside, macrolide, moxifloxacin resistance, respectively. This study highlights the importance of precise identification of clinical isolates genetically and suggests pay great attention to the lung disease caused by M. indicus pranii.