AUTHOR=Ruhs Emily Cornelius , Chia Wan Ni , Foo Randy , Peel Alison J. , Li Yimei , Larman H. Benjamin , Irving Aaron T. , Wang Linfa , Brook Cara E. TITLE=Applications of VirScan to broad serological profiling of bat reservoirs for emerging zoonoses JOURNAL=Frontiers in Public Health VOLUME=Volume 11 - 2023 YEAR=2023 URL=https://www.frontiersin.org/journals/public-health/articles/10.3389/fpubh.2023.1212018 DOI=10.3389/fpubh.2023.1212018 ISSN=2296-2565 ABSTRACT=We present the first full-application of Phage ImmunoPrecipitation Sequencing (PhIP-Seq) technology in a bat system, to describe the history of viral exposure in semi-captive populations of Pteropus alecto and Eonycteris spelaea fruit bats. Our paper provides the first record of antibody responses to several previously-unreported viruses in P. alecto serum, including alphaviruses, parvoviruses, and orthopoxviruses and offers a uniquely comprehensive window into associations between past viral exposures and bat health. As bats are largely assumed to be tolerant of most viruses, documentation of the subtle morbidity effects of infection is important. While prior studies have focused primarily on applications of PhIP-Seq to profiling antiviral immunity in humans and mice, here, we outline recommendations for its application to wildlife species. PhIP-Seq technology holds exciting potential as a tool to facilitate future surveillance efforts for potentially zoonotic viruses in wildlife reservoirs, including bats.