AUTHOR=Li Yulian , Bian Wentao , Wu Shiping , Zhang Jie , Li Dan 

TITLE=Metagenomic next-generation sequencing for Mycobacterium tuberculosis complex detection: a meta-analysis

JOURNAL=Frontiers in Public Health

VOLUME=Volume 11 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/public-health/articles/10.3389/fpubh.2023.1224993

DOI=10.3389/fpubh.2023.1224993

ISSN=2296-2565

ABSTRACT=Objective: Metagenomic next-generation sequencing (mNGS) has been gradually applied to the diagnosis of tuberculosis (TB) due to its rapid and highly sensitive characteristics. Despite numerous studies on this subject, their results vary significantly. Thus, the current meta-analysis was performed to assess the performance of the mNGS on tuberculosis. Methods: PubMed, Embase, Web of science, and The Cochrane Library were searched up to June 21, 2023. Studies utilizing the mNGS for tuberculosis detection were included. The risk of bias was assessed by QUADAS-2, and a meta-analysis was performed with STATA14.0 software. Results: 17 studies comprising 3205 specimens were included. The combined sensitivity and specificity of mNGS for clinical specimens were 0.