AUTHOR=Bentley Tanya G. K. , Seeber Cerena , Hightower Emily , Mackenzie Brian , Wilson Rob , Velazquez Aly , Cheng Anna , Arce Nicholas N. , Lorenz Kent A. TITLE=Slow-Breathing Curriculum for Stress Reduction in High School Students: Lessons Learned From a Feasibility Pilot JOURNAL=Frontiers in Rehabilitation Sciences VOLUME=Volume 3 - 2022 YEAR=2022 URL=https://www.frontiersin.org/journals/rehabilitation-sciences/articles/10.3389/fresc.2022.864079 DOI=10.3389/fresc.2022.864079 ISSN=2673-6861 ABSTRACT=Purpose Nearly 1 in 3 US adolescents meet the criteria for anxiety, an issue that has worsened with the COVID-19 pandemic. We developed a video-based slow diaphragmatic breathing stress-reduction curriculum for high school students and evaluated its feasibility, tolerability, and preliminary effectiveness. Methods This cluster-randomized feasibility pilot compared 5-minute slow diaphragmatic breathing for 5 weeks with treatment-as-usual control among four 12th-grade public high school classes. Students individually participated after school during COVID-19-related hybrid teaching, with slow diaphragmatic breathing 3 times/week and breath science education once/week. Feasibility was based on completion of breathing exercises, breath science education, and preliminary effectiveness assessments, and ease/tolerability was based on qualitative assessments. Preliminary effectiveness was measured with the State-Trait Anxiety Inventory (STAI) and a timed-exhale carbon dioxide tolerance test (CO2TT) of physiological stress response. Descriptive statistics and repeated analysis of variance were performed to quantify and compare outcomes between time periods. Human subjects research approval was granted through Western IRB - Copernicus Group (WCG IRB). [ClinicalTrials.gov Identifier: NCT05266833] Results Forty-three students consented to participate. Breath practice compliance ranged from 29% to 83% across classes and weeks, and decreased on average over the 5 weeks. Compliance with the breath science videos ranged from 43% to 86%, and that with the weekly STAI-State and CO2TT measures varied from 36% to 86%. Compliance with ease/tolerability assessments ranged from 0-60%. Preliminary effectiveness assessments’ compliance varied across classes from 83%-89% during baseline, and 29%-72% at follow-up. The curriculum was rated as somewhat-to-definitely useful/beneficial, and definitely-to-very easy/tolerable. Students reported enjoying the diaphragmatic breathing, CO2TT, and breath science education; some found the extended exhales challenging and the curriculum and assessments time-consuming. Preliminary effectiveness analyses indicated no significant changes in STAI or CO2TT from baseline to followup or from before to after breathing exercises (p>0.05 for all). Conclusions Implementation of this 5-week slow breathing curriculum was feasible and tolerable to this cohort. Compliance, tolerability, and effectiveness may be improved with in-class participation. Future research on simple and accessible slow-breathing exercises is warranted to address today’s adolescent stress-management crisis.