AUTHOR=Fernandez-Gnecco Gabriela , Covacevich Fernanda , Consolo Veronica F. , Behr Jan H. , Sommermann Loreen , Moradtalab Narges , Maccario Lorrie , Sørensen Søren J. , Deubel Annette , Schellenberg Ingo , Geistlinger Joerg , Neumann Günter , Grosch Rita , Smalla Kornelia , Babin Doreen 

TITLE=Effect of Long-Term Agricultural Management on the Soil Microbiota Influenced by the Time of Soil Sampling

JOURNAL=Frontiers in Soil Science

VOLUME=Volume 2 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/soil-science/articles/10.3389/fsoil.2022.837508

DOI=10.3389/fsoil.2022.837508

ISSN=2673-8619

ABSTRACT=Application of agrochemicals and mechanization enabled to increase agricultural productivity yet caused various environmental and soil health-related problems. Agricultural practices affect soil microorganisms which are the key players of many ecosystem processes. However, less is known whether this effect differs between time points. To achieve this goal, soil was sampled in winter (without crop) and in summer (presence of maize) from a long-term field experiment (LTE) in Bernburg (Germany) managed either under cultivator tillage (CT) or mouldboard plough (MP) in combination with either intensive nitrogen (N)-fertilization and pesticides (Int) or extensive reduced N- fertilization without fungicides (Ext), respectively. High-throughput sequencing of 16S rRNA gene and fungal ITS2 amplicons showed that changes in the microbial community composition were correlated to differences in soil chemical properties caused by tillage practice. Microbial communities of soils sampled in winter differed only depending on the tillage practice, while in summer also a strong effect of the fertilization intensity was observed. A small proportion of microbial taxa was shared between soils from the two sampling times, suggesting the existence of a stable core microbiota at the LTE. In general, taxa associated with organic matter decomposition (such as Actinobacteria, Bacteroidetes, Rhizopus and Exophiala) had a higher relative abundance under CT. Among the taxa with significant changes in relative abundances due to different long-term agricultural practices were putative pathogenic (e.g., Gibellulopsis and Gibberella) and beneficial microbial genera (e.g., Chitinophagaceae, Ferruginibacter, Minimedusa). In summary, this study suggests that the effects of long-term agricultural management practices on the soil microbiota are influenced by the soil sampling time which need to be kept in mind in future studies for the interpretation of field data