AUTHOR=van der Zwaard Stephan , Brocherie Franck , Jaspers Richard T. TITLE=Under the Hood: Skeletal Muscle Determinants of Endurance Performance JOURNAL=Frontiers in Sports and Active Living VOLUME=Volume 3 - 2021 YEAR=2021 URL=https://www.frontiersin.org/journals/sports-and-active-living/articles/10.3389/fspor.2021.719434 DOI=10.3389/fspor.2021.719434 ISSN=2624-9367 ABSTRACT=In the past decades, researchers have extensively studied (elite) athletes’ physiological responses to understand how to maximize their endurance performance. In endurance sports, whole-body measurements such as the maximal oxygen consumption, lactate threshold and efficiency/economy play a key role in performance. Although these determinants are known to interact, it has also been demonstrated that athletes rarely excel in all three. The leading question is how athletes reach exceptional values in one or all of these determinants to optimise their endurance performance, and how such performance can be explained by (combinations of) underlying physiological determinants. In this review, we advance on Joyner and Coyle’s conceptual framework of endurance performance, by integrating a meta-analysis of the interrelationships and corresponding effect sizes between endurance performance and its key physiological determinants at the macroscopic (whole-body) and the microscopic level (muscle tissue, i.e. muscle fibre oxidative capacity, oxygen supply, muscle fibre size and fibre type). Moreover, we discuss how these physiological determinants can be improved by training and what potential physiological challenges endurance athletes may face when trying to maximize their performance. This review highlights that integrative assessment of skeletal muscle determinants points towards efficient type-I fibres with a high mitochondrial oxidative capacity and strongly encourages well-adjusted capillarization and myoglobin concentrations to accommodate the required oxygen flux during endurance performance, especially in large muscle fibres. Optimisation of endurance performance requires careful design of training interventions that finetune modulation of exercise intensity, frequency and duration, and particularly periodisation with respect to the skeletal muscle determinants.