AUTHOR=Lv Xiaofei , Yu Xi 

TITLE=Signatures and prognostic values of related immune targets in tongue cancer

JOURNAL=Frontiers in Surgery

VOLUME=Volume 9 - 2022

YEAR=2023

URL=https://www.frontiersin.org/journals/surgery/articles/10.3389/fsurg.2022.952389

DOI=10.3389/fsurg.2022.952389

ISSN=2296-875X

ABSTRACT=Background
Tongue cancer, as one of the most malignant oral cancers, is highly invasive and has a high risk of recurrence. At present, tongue cancer is not obvious and easy to miss the opportunity of early diagnosis when in the advanced stage. It is important to find markers that can predict the occurrence and progression of tongue cancer. 
Methods
Bioinformatics analysis plays an important role in the acquisition of marker genes. GEO and TCGA data are very important public databases. In addition to expression data, TCGA database also contains corresponding clinical data. In this study, we screened three GEO datasets included GSE13601, GSE34105 and GSE34106 that met the standard. These data sets were combined using the SVA package to prepare the data for differential expression analysis, and then the LIMMA package was used to set the standard to p<0.05 and |log2 (FC)| ≥1.5. 
Results
The 170 differentially expressed genes (DEGs) were identified. Besides, the DEseq package was used for differential expression analysis using the same criteria for samples in TCGA database. It ended up with 1589 DEGs (644 up-regulated, 945 down-regulated). By merging these two sets of DEGs, 5 common up-regulated DEGs (CCL20, SCG5, SPP1, KRT75 and FOLR3) and 15 common down-regulated DEGs were obtained. 
Conclusions
Further functional analysis of the DEGs showed that CCL20, SCG5 and SPP1 is closely related to prognosis and may be a therapeutic target of TSCC.