AUTHOR=Chen Jiahuan , Feng Chenwei , Guo Xiao , Zhou Yuchen , Gu Tianxiao , Zhuang Xinjian , Cheng Lei , Zhang Kun 

TITLE=Development of polyclonal antibodies-based serological methods for detection of the rehmannia mosaic virus in field plants

JOURNAL=Frontiers in Sustainable Food Systems

VOLUME=Volume 6 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/sustainable-food-systems/articles/10.3389/fsufs.2022.1013470

DOI=10.3389/fsufs.2022.1013470

ISSN=2571-581X

ABSTRACT=We explored the occurrence, damage, and genetic variation of Rehmannia mosaic virus (ReMV) in samples from Henan Province, China. Using preserved samples of Rehmannia glutinosa carrying ReMV from a previous study. Total RNA was extracted using phenol-chloroform, and the CPReMV opening reading frame was cloned and sequenced. The target sequence was further placed into a prokaryotic expression vector to express the N-terminal-tagged recombinant CPReMV protein (His-CPReMV). Purified His-CPReMV was used as an antigen to immunize New Zealand white rabbits, and antiserum was obtained. The titers and sensitivities of the antisera were analyzed and evaluated. Polyclonal antibodies were purified from the antiserum, and the titers and sensitivity to the target His-CPReMV protein were evaluated. The results demonstrate that the obtained polyclonal antibodies against His-CPReMV could be successfully used for rapid and efficient detection of ReMV from R. glutinosa planted in the wild. Our investigation established serological-based detection methods for ReMV for the first time, and provides a foundation for future exploration of the pathogenic mechanisms of ReMV in R. glutinosa.