AUTHOR=Wu Xiyang , Chen Yue , Chen Chunyan , Huang Qiang , Qin Yingfei , Zhang Xin , Li Chenggang , Tan Xinqiu , Liu Yong , Zhang Deyong 

TITLE=Combining the microbial agent Rhodopseudomonas palustris strain PSB-06 with fungicides for controlling rice blast

JOURNAL=Frontiers in Sustainable Food Systems

VOLUME=Volume 6 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/sustainable-food-systems/articles/10.3389/fsufs.2022.1072156

DOI=10.3389/fsufs.2022.1072156

ISSN=2571-581X

ABSTRACT=The rice blast disease caused by Magnaporthe oryzae threatens global rice production yields. The photosynthetic bacterium Rhodopseudomonas palustris (PSB-06) significantly antagonizes rice blast. Tricyclazole and isoprothiolane are widely used fungicides with high activity against rice blast. However, the effect of combining these two chemical fungicides with PSB-06 on rice blast control is unclear. First, we detected the Isoprothiolane and tricyclazole have significant inhibitory effects on Magnaporthe oryzae, The sensitivity of PSB-06 to tricyclazole and isoprothiolane was then tested. The growth of PSB-06 was unaffected by up to 1.25 mg/L of tricyclazole and 0.3 mg/L of isoprothiolane in the photosynthetic medium. While the concentration of the two fungicides was 5 mg/L, the rate of appressorium germination was only 7%. When PSB-06 was combined with isoprothiolane, the leaf lesion density was the lowest, and the control efficiency in the field test reached 76.06%. This value was significantly higher than the individual efficiency of PSB-06 (67.99%) and tricyclazole (65.46%) and the combined control efficiency (72.20%) of these two chemicals. Our current findings highlighted the potential of combining R. palustris strain PSB-06 with isoprothiolane to control rice blast, thereby providing environmental protection and reducing the use of fungicides.