AUTHOR=Yang Miaoling , Han Xiaoqing , Xie Jiabei , Zhang Shangqing , Lv Zhaoyang , Li Boya , Shi Liming , Zhang Kecheng , Ge Beibei TITLE=Field Application of Wuyiencin Against Sclerotinia Stem Rot in Soybean JOURNAL=Frontiers in Sustainable Food Systems VOLUME=Volume 6 - 2022 YEAR=2022 URL=https://www.frontiersin.org/journals/sustainable-food-systems/articles/10.3389/fsufs.2022.930079 DOI=10.3389/fsufs.2022.930079 ISSN=2571-581X ABSTRACT=Sclerotinia stem rot, caused by Sclerotinia sclerotiorum, is a devastating disease of soybean. Biological control is a potential alternative to chemical fungicides for disease management, and provides broad benefits to the environment, farmers and consumers. Herein, we established a field application technique for biocontrol of Sclerotinia stem rot in soybean using wuyiencin, expanding on a previous study showing biocontrol potential. We used wuyiencin to reduce sclerotia in soybean seed, and disease incidence analysis by seed bioassay revealed an optimal wuyiencin seed soaking concentration of 12.5 µg/mL. We found that different application methods had different effects on soybean plant growth. Soybean pot experiments showed that soybean roots were sensitive to wuyiencin, especially at high concentrations, and the optimal concentration for wuyiencin spraying was 100200 µg/mL. We tested the efficacy of applying wuyiencin under field conditions, and the control effect of 200 µg/mL wuyiencin sprayed three times was the best (64.0%), but this was slightly inferior to the control effect of 200 µg/mL dimethachlon (77.6%).