AUTHOR=Sharma Shakshi , Sharma Nivedita , Kaushal Neha 

TITLE=Utilization of novel bacteriocin synthesized silver nanoparticles (AgNPs) for their application in antimicrobial packaging for preservation of tomato fruit

JOURNAL=Frontiers in Sustainable Food Systems

VOLUME=Volume 7 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/sustainable-food-systems/articles/10.3389/fsufs.2023.1072738

DOI=10.3389/fsufs.2023.1072738

ISSN=2571-581X

ABSTRACT=Developing safe packaging system that maintains quality of food and prevent its spoilage is the current demand of food industries. In the present study an attempt had been made to obtain purified bacteriocin from 3 potential bacteriocinogenic probiotic isolates i.e., Lactobacillus pentosus S6 (KU92122), Lactobacillus crustorum F11 (KT865221) and Lactobacillus spicheri G2 (JX481912) to synthesize silver nanoparticles (AgNPs) and their usage in antimicrobial coating of cellulose paper. The antimicrobial efficacy of crude, partially purified and purified bacteriocin was tested against food borne spoilage causing pathogens i.e. Bacillus cereus and Staphylococcus aureus. The purified bacteriocin obtained was used for the synthesis of AgNPs. Further, the AgNPs formation was confirmed by using UV-vis spectroscopy, Scanning Electron Microscopy (SEM) and Transmission electron Microscopy (TEM) technique. Bacteriocin synthesized AgNPs was then used for coating of cellulose paper with a main motive to avoid spoilage and enhance shelf stability of tomato fruit during storage. SEM analysis confirmed the coating of AgNPs in the cellulose paper. The enhanced antimicrobial efficacy of coated paper was observed against Bacillus cereus and Staphylococcus aureus. The AgNPs coated paper along with silver nitrate (AgNO3) coated paper and uncoated paper was used for the storage of tomato fruit for a period of 10 days in room temperature. Changes during storage were determined by analyzing morphological and color changes. Tomato fruit stored in AgNPs coated paper maintained and retained its appearance and firmness as compared to the AgNO3 coated and uncoated paper.