AUTHOR=Rivera-Solís Rodrigo A. , Granados-Baeza Manuel J. , Solís-Pereira Sara E. , Lizama-Uc Gabriel , Magaña-Ortiz Denis , Sánchez-González Mónica N. , Rojas-Herrera Rafael A. , Rivera-Muñoz Gerardo 

TITLE=Establishment of the colloidal chitin enzymatic hydrolysis conditions to obtain N-acetyl glucosamine

JOURNAL=Frontiers in Sustainable Food Systems

VOLUME=Volume 7 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/sustainable-food-systems/articles/10.3389/fsufs.2023.1077429

DOI=10.3389/fsufs.2023.1077429

ISSN=2571-581X

ABSTRACT=Establishment of the colloidal chitin enzymatic hydrolysis conditions to obtain N-acetyl glucosamine.
Rivera-Solís, R.A.2,  Granados-Baeza, M.1. Solís-Pereira S. E.1, Lizama-Uc, G.1, Ortiz-Magaña, D.1, Sánchez-González, M.N.2, Rojas-Herrera, R.A.2, Rivera-Muñoz, G1.

1) Instituto Tecnológico de Mérida/Tecnológico Nacional de México, Departamento de Ingeniería Química y Bioquímica. Km. 5 Carr. Mérida-Progreso S.N., Mérida, Yucatán México
2)  Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Periférico norte Km. 33.5, tablaje catastral 13615, Chuburna de Hidalgo Inn, C.P. 97203. 

The waste generated by the shrimp industry represents between 50 and 60% of the catch volume. This percentage corresponds to the head, viscera, and shell. These residues are potential pollutants if they are not given proper disposal and treatment. One way to solve the problem associated with the pollution generated by this type of waste is its use to obtain functional foods. In this sense, shrimp waste is rich in chitin, which is considered the second most abundant biopolymer on the planet, only after cellulose. The hydrolysis of chitin gives N-acetyl glucosamine as a final product that can be used as a sweetener in the food sector and as an aid in the treatment of coronary diseases and gonarthrosis. This feature allows us to glimpse the potential of the shrimp waste to be used as raw material to obtain N-acetyl glucosamine to be used as a functional food.
In this work, we describe the study conducted by our group to set up the enzymatic hydrolysis conditions for obtaining N-acetyl glucosamine, using an enzymatic filtrate produced by a marine bacterium isolated in the coastal zone of Progreso, Yucatan, Mexico.
The best N-acetyl glucosamine titer, 265 µg/mL, (2.65%) was obtained using 10000 µg/mL colloidal chitin. The conditions to achieve this were: a temperature of 60 oC and a pH of 8.9.