AUTHOR=Zhao Yixuan , Guo Junhong , Luo Shiheng , Shen Yuying , Siddique Kadambot H. M. , Li Yuan TITLE=Optimizing lucerne (Medicago sativa) termination on the Loess Plateau, China: a comparative analysis of conventional tillage and herbicide treatments JOURNAL=Frontiers in Sustainable Food Systems VOLUME=Volume 7 - 2023 YEAR=2023 URL=https://www.frontiersin.org/journals/sustainable-food-systems/articles/10.3389/fsufs.2023.1194724 DOI=10.3389/fsufs.2023.1194724 ISSN=2571-581X ABSTRACT=Lucerne (Medicago sativa L.) is a crucial component in agricultural rotation systems due to its ability to enhance soil carbon (C) and nitrogen (N) contents. In China, conventional tillage (CT) is commonly used for lucerne termination, leading to soil structure degradation and C and N losses. While herbicide application has been suggested for lucerne termination in the USA and EU, its impact on lucerne growth suppression and soil C and N contents in China remains uncertain. In this study, we examined the effects of herbicide type [glyphosate and 2,4-D (G+2), glyphosate and dicamba (G+D), 2,4-D and dicamba (2+D), with water (W) and CT as controls] and concentration (100%, 200%, 300%) on various lucerne and soil properties. Our findings revealed that G+2 and 2+D treatments significantly reduced the regreening rate, and the recommended herbicide concentration (100%) was sufficient for lucerne termination. CT and W treatments resulted in a higher soil pH compared to herbicide treatments. Moreover, herbicide treatments exhibited higher soil organic C (SOC) and total N (TN) levels than the CT treatment. The 2+D treatment demonstrated a higher SOC content compared to the G+2 treatment, while the 200% and 300% herbicide concentrations reduced SOC and TN levels. The G+2 treatment had the highest soil ammonium nitrogen content (7.94±1.45 mg kg–1), while the CT treatment showed the lowest (6.46±1.54 mg kg–1). In conclusion, our study suggests that applying the recommended herbicide dosage (100%) effectively terminates lucerne grassland on China's Loess Plateau without negatively impacting soil C and N storage.