AUTHOR=Zhang Yansong , Ding Yihan , Ma Yidan , Zhang Zixin , Wang Yipei , Li Daojin , Wang Shuangshou 

TITLE=High-capacity boronate affinity-based template-immobilized surface imprinted silica nanoparticles for rapid, selective, and efficient extraction and determination of lincomycin in milk and chicken

JOURNAL=Frontiers in Sustainable Food Systems

VOLUME=Volume 7 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/sustainable-food-systems/articles/10.3389/fsufs.2023.1271921

DOI=10.3389/fsufs.2023.1271921

ISSN=2571-581X

ABSTRACT=Background: Lincomycin, a natural antibiotic, is widely used by animal and fishery husbandries to prevent infections and treat diseases. It brings threat to the people's health when they eat these foods containing lincomycin residue, especial frequent milk and chicken consumption. Thus, it is extremely important to evaluate the content of lincomycin in food samples. However, the direct analysis of lincomycin in milk and chicken is quite difficult because of its very low concentration level and the presence of undesirable matrix effects. Thus, selective and efficient extraction of lincomycin from complex food samples prior to its quantification is required.Results: In this study, the lincomycin-imprinted silica nanoparticles were prepared according to boronate affinity-based template-immobilized surface imprinting. Silica nanoparticles and boronic acid ligands 3-fluoro-4-formylphenylboronic acid were used as supporting materials and functional monomer, respectively. The prepared lincomycin-imprinted silica nanoparticles exhibited several significant results, such as good specificity, high binding capacity (19.45 mg/g), fast kinetics (6 min) and low binding pH (pH 5.0) toward lincomycin. The reproducibility of lincomycin-imprinted silica nanoparticles was satisfactory. The lincomycin-imprinted silica nanoparticles could be still reused after seven adsorption-desorption cycles, which indicated high chemical stability. In addition, the recoveries of the proposed method for lincomycin at three spiked levels analysis in milk and chicken were 93.3% to 103.3% and 90.0% to 100.0%, respectively.The prepared lincomycin-imprinted silica nanoparticles are feasible for the recognition of target lincomycin with low concentration in real food samples milk and chicken. Our approach makes sample pre-preparation simple, fast, selective and efficient.