AUTHOR=Tewari Kalpana , Kumar Vaibhav , Parihar Ashok Kumar , Jha Sudhir Kumar , Kumar Kuldeep , Kumar Rishikesh , Deo Man Mohan , Meena Surendra Kumar , Kumar Rajeev , Senthil Kumar M. , Dixit Girish Prasad 

TITLE=Characterisation of the α-GOS profile of field pea and cowpea cultivars and their modulation through sustainable processing methods

JOURNAL=Frontiers in Sustainable Food Systems

VOLUME=Volume 9 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/sustainable-food-systems/articles/10.3389/fsufs.2025.1600726

DOI=10.3389/fsufs.2025.1600726

ISSN=2571-581X

ABSTRACT=α-galactooligosaccharides (α-GOS) are a class of non-nutritional compounds present in pulses. These carbohydrates are associated with irritable bowel syndrome (IBS). This study explored the genotypic variability for α-GOS content in field pea and cowpea cultivars using high-performance liquid chromatography coupled with a refractive index detector (HPLC-RI). Verbacose, stachyose and raffinose were detected in field pea, whereas in cowpea, only stachyose and raffinose were detected, except the cultivar RC 101 in which verbascose was also found. The total α-GOS content varied from 65.89 ± 0.21 mg/g DW to 101.84 ± 0.33 mg/g DW and 78.78 ± 0.65 mg/g DW to 108.00 ± 0.49 mg/g DW in field pea and cowpea, respectively. The cultivars IPFD 16–13 and KBC 7 were identified as low α-GOS cultivars of field pea and cowpea, respectively. Further, three low-cost, sustainable processing methods, viz. soaking, cooking, and malting (12, 24, 48 and 72 h), were investigated for their effect on the α-GOS content of field pea and cowpea. The results from this study show that malting with 72 h of germination emerged as the most effective method in reducing the total α-GOS content. It reduced verbascose and stachyose content by 74.6 and 67.43% in field pea and by 44.82 and 69.64% in cowpea.