AUTHOR=Angarita-Rodríguez Andrea , Mendoza-Mejía Nicolás , González Janneth , Papin Jason , Aristizábal Andrés Felipe , Pinzón Andrés 

TITLE=Improvement in the prediction power of an astrocyte genome-scale metabolic model using multi-omic data

JOURNAL=Frontiers in Systems Biology

VOLUME=Volume 4 - 2024

YEAR=2025

URL=https://www.frontiersin.org/journals/systems-biology/articles/10.3389/fsysb.2024.1500710

DOI=10.3389/fsysb.2024.1500710

ISSN=2674-0702

ABSTRACT=IntroductionThe availability of large-scale multi-omic data has revolution-ized the study of cellular machinery, enabling a systematic understanding of biological processes. However, the integration of these datasets into Genome-Scale Models of Metabolism (GEMs) re-mains underexplored. Existing methods often link transcriptome and proteome data independently to reaction boundaries, providing models with estimated maximum reaction rates based on individual datasets. This independent approach, however, introduces uncertainties and inaccuracies.MethodsTo address these challenges, we applied a principal component analysis (PCA)-based approach to integrate transcriptome and proteome data. This method facilitates the reconstruction of context-specific models grounded in multi-omics data, enhancing their biological relevance and predictive capacity.ResultsUsing this approach, we successfully reconstructed an astrocyte GEM with improved prediction capabilities compared to state-of-the-art models available in the literature.DiscussionThese advancements underscore the potential of multi-omic inte-gration to refine metabolic modeling and its critical role in studying neurodegeneration and developing effective therapies.