AUTHOR=Aonuma Hiroka , Iizuka Itoe , Li Jian-Chiuan , Ote Manabu , Tajima Shigeru , Saijo Masayuki , Chen Chun-Hong , Kanuka Hirotaka 

TITLE=LAMP Detection of Virus-Derived DNA of Zika Virus in Vector Mosquito

JOURNAL=Frontiers in Tropical Diseases

VOLUME=Volume 3 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/tropical-diseases/articles/10.3389/fitd.2022.759375

DOI=10.3389/fitd.2022.759375

ISSN=2673-7515

ABSTRACT=Detection of infectious viruses in mosquitoes is one of the prerequisite measures to monitor the prevalence of vector-borne viral diseases. Determining which mosquito is presently infected with an arbovirus such as Zika, dengue, and chikungunya viruses is still not practical in endemic areas due to multiple causes including difficulty in dealing with the unstable RNA of the virus in the field. In this study, instead of handling viral RNA, virus-derived DNA (vDNA), which is a newly discovered DNA form of arbovirus generated in insect cells, was introduced as a target template for nucleic acid amplification. In combination with loop-mediated isothermal amplification (LAMP), we examined a LAMP-based vDNA detection assay (vDNA-LAMP) targeting Zika virus (ZIKV). The vDNA-LAMP reaction amplifying part of the NS3 region of ZIKV successfully detected its vDNA from crude DNA purified from artificially infected cultured cells and Aedes mosquitoes. This rapid, simple, and versatile method may provide a promising field-surveillance method for arbovirus circulation via vector mosquitoes.