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<front>
<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Tuberc.</journal-id>
<journal-title>Frontiers in Tuberculosis</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Tuberc.</abbrev-journal-title>
<issn pub-type="epub">2813-7868</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="doi">10.3389/ftubr.2024.1378068</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Tuberculosis</subject>
<subj-group>
<subject>Review</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Diverse interactions of <italic>Mycobacterium tuberculosis</italic> infection and of BCG vaccination with SARS-CoV-2</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author" corresp="yes">
<name><surname>Salgame</surname> <given-names>Padmini</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
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<contrib contrib-type="author" equal-contrib="yes">
<name><surname>Pentakota</surname> <given-names>Sri Ram</given-names></name>
<xref ref-type="aff" rid="aff2"><sup>2</sup></xref>
<xref ref-type="author-notes" rid="fn002"><sup>&#x02020;</sup></xref>
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<contrib contrib-type="author" equal-contrib="yes">
<name><surname>Malabad</surname> <given-names>John Carlo M.</given-names></name>
<xref ref-type="aff" rid="aff3"><sup>3</sup></xref>
<xref ref-type="aff" rid="aff4"><sup>4</sup></xref>
<xref ref-type="author-notes" rid="fn002"><sup>&#x02020;</sup></xref>
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<name><surname>Narasimhan</surname> <given-names>Prakash Babu</given-names></name>
<xref ref-type="aff" rid="aff5"><sup>5</sup></xref>
<xref ref-type="author-notes" rid="fn002"><sup>&#x02020;</sup></xref>
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<contrib contrib-type="author" equal-contrib="yes">
<name><surname>Verma</surname> <given-names>Sheetal</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
<xref ref-type="author-notes" rid="fn002"><sup>&#x02020;</sup></xref>
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<contrib contrib-type="author" equal-contrib="yes">
<name><surname>Prakash Babu</surname> <given-names>Senbagavalli</given-names></name>
<xref ref-type="aff" rid="aff6"><sup>6</sup></xref>
<xref ref-type="author-notes" rid="fn002"><sup>&#x02020;</sup></xref>
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<name><surname>Sharma</surname> <given-names>Vartika</given-names></name>
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<name><surname>Sarkar</surname> <given-names>Sonali</given-names></name>
<xref ref-type="aff" rid="aff6"><sup>6</sup></xref>
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<contrib contrib-type="author">
<name><surname>Alejandria</surname> <given-names>Marissa M.</given-names></name>
<xref ref-type="aff" rid="aff3"><sup>3</sup></xref>
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<name><surname>Ellner</surname> <given-names>Jerrold J.</given-names></name>
<xref ref-type="aff" rid="aff1"><sup>1</sup></xref>
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<aff id="aff1"><sup>1</sup><institution>Department of Medicine, Center for Emerging Pathogens, Rutgers-New Jersey Medical School</institution>, <addr-line>Newark, NJ</addr-line>, <country>United States</country></aff>
<aff id="aff2"><sup>2</sup><institution>Department of Medicine, Rutgers New Jersey Medical School</institution>, <addr-line>Newark, NJ</addr-line>, <country>United States</country></aff>
<aff id="aff3"><sup>3</sup><institution>Institute of Clinical Epidemiology, National Institutes of Health, University of the Philippines Manila</institution>, <addr-line>Manila</addr-line>, <country>Philippines</country></aff>
<aff id="aff4"><sup>4</sup><institution>Department of Science and Technology, DOST Compound</institution>, <addr-line>Taguig</addr-line>, <country>Philippines</country></aff>
<aff id="aff5"><sup>5</sup><institution>Department of Clinical Immunology, Jawaharlal Institute of Postgraduate Medical Education and Research</institution>, <addr-line>Puducherry</addr-line>, <country>India</country></aff>
<aff id="aff6"><sup>6</sup><institution>Department of Preventive and Social Medicine, Jawaharlal Institute of Postgraduate Medical Education and Research</institution>, <addr-line>Puducherry</addr-line>, <country>India</country></aff>
<author-notes>
<fn fn-type="edited-by"><p>Edited by: Elsa Anes, University of Lisbon, Portugal</p></fn>
<fn fn-type="edited-by"><p>Reviewed by: Paulo J. G. Bettencourt, Universidade Cat&#x000F3;lica Portuguesa, Portugal</p>
<p>Hridayesh Prakash, Amity Centre for Translational Research, India</p></fn>
<corresp id="c001">&#x0002A;Correspondence: Padmini Salgame <email>salgampa&#x00040;njms.rutgers.edu</email></corresp>
<corresp id="c002">Jerrold J. Ellner <email>ellnerjj&#x00040;njms.rutgers.edu</email></corresp>
<fn fn-type="equal" id="fn002"><p>&#x02020;These authors have contributed equally to this work</p></fn></author-notes>
<pub-date pub-type="epub">
<day>23</day>
<month>04</month>
<year>2024</year>
</pub-date>
<pub-date pub-type="collection">
<year>2024</year>
</pub-date>
<volume>2</volume>
<elocation-id>1378068</elocation-id>
<history>
<date date-type="received">
<day>29</day>
<month>01</month>
<year>2024</year>
</date>
<date date-type="accepted">
<day>02</day>
<month>04</month>
<year>2024</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#x000A9; 2024 Salgame, Pentakota, Malabad, Narasimhan, Verma, Prakash Babu, Sharma, Sarkar, Alejandria and Ellner.</copyright-statement>
<copyright-year>2024</copyright-year>
<copyright-holder>Salgame, Pentakota, Malabad, Narasimhan, Verma, Prakash Babu, Sharma, Sarkar, Alejandria and Ellner</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/"><p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</p></license>
</permissions>
<abstract>
<p>The COVID pandemic and tuberculosis (TB) endemicity is double trouble to much of the world. SARS-CoV-2 and <italic>Mycobacterium tuberculosis</italic> (Mtb), causative agents of COVID and TB, respectively, are both infectious respiratory pathogens involving close communities and individuals. Both pathogens can cause lung disease, involving unbalanced inflammatory cell immune responses that can lead to a syndemic impact. Moreover, dual infection is common in certain settings. In low- and middle- income countries, most individuals with SARS-CoV-2 infection or COVID-19, in fact, will have been exposed to or infected with Mtb and some will develop active TB. Here we review the literature examining the diverse interactions of <italic>M. tuberculosis</italic> infection and of BCG vaccination with SARS-CoV-2. We discuss areas in which contradictory results have been published and conclude that there are still several unresolved issues that warrant further study on the co-pathogenesis of SARS-CoV-2 and Mtb and BCG- mediated heterologous protection against COVID-19.</p></abstract>
<kwd-group>
<kwd>SARS-CoV-2</kwd>
<kwd>tuberculosis</kwd>
<kwd>trained immunity</kwd>
<kwd>COVID-19</kwd>
<kwd><italic>Mycobacterium tuberculosis</italic></kwd>
</kwd-group>
<counts>
<fig-count count="2"/>
<table-count count="0"/>
<equation-count count="0"/>
<ref-count count="105"/>
<page-count count="9"/>
<word-count count="7802"/>
</counts>
<custom-meta-wrap>
<custom-meta>
<meta-name>section-at-acceptance</meta-name>
<meta-value>Pathogen and Host Biology of Tuberculosis</meta-value>
</custom-meta>
</custom-meta-wrap>
</article-meta>
</front>
<body>
<sec sec-type="intro" id="s1">
<title>Introduction</title>
<p>The clinical, epidemiologic, and biologic interactions of <italic>Mycobacterium tuberculosis</italic> (Mtb) infection (MTBI), and of BCG vaccination with SARS-CoV-2 infection and may be substantial, diverse and bidirectional. SARS-CoV-2 and <italic>Mycobacterium tuberculosis</italic> (Mtb), causative agents of COVID and TB, respectively, are both infectious respiratory pathogens involving close communities and individuals. Both pathogens can cause lung disease, involving unbalanced inflammatory cell immune responses that can lead to a syndemic impact. (<xref ref-type="bibr" rid="B1">1</xref>). Moreover, dual infection is common in certain settings. In low- and middle- income countries, most individuals with SARS-CoV-2 infection or COVID-19, in fact, will have been infected with Mtb by adolescence (<xref ref-type="bibr" rid="B2">2</xref>, <xref ref-type="bibr" rid="B3">3</xref>) and some will develop active tuberculosis (TB). COVID-19 also may occur before TB particularly with the nearly ubiquitous frequency of SARS-CoV-2 in the population (<xref ref-type="bibr" rid="B3">3</xref>, <xref ref-type="bibr" rid="B4">4</xref>). Therefore, it is important to consider interactions that may be impacted by the order of acquisition of these pathogens and the stage and severity of the resultant infection and disease.</p>
<p>There may be relevant lessons from the interactions of Mtb and HIV-1 (<xref ref-type="bibr" rid="B5">5</xref>). The pandemic of HIV-1 infection had profound impact on TB (<xref ref-type="bibr" rid="B6">6</xref>). A surge in new cases of TB was the harbinger of a new, at the time, unknown infectious agent spreading in Africa. HIV-1 affected the natural history, manifestations, and course of TB. It soon became apparent that TB also affected the course of HIV-1 infection, promoting viral replication and accelerating progression to immunodeficiency (<xref ref-type="bibr" rid="B7">7</xref>, <xref ref-type="bibr" rid="B8">8</xref>). The impact on TB on other co-infections such as malaria, Middle East respiratory syndrome (MERS), and severe acute respiratory syndrome (SARS) has been addressed (<xref ref-type="bibr" rid="B5">5</xref>, <xref ref-type="bibr" rid="B9">9</xref>&#x02013;<xref ref-type="bibr" rid="B11">11</xref>).</p>
<p>There are similarities and overlap in the pathogenic mechanisms of Mtb and of SARS-CoV-2. Each is spread by the aerosol route and engages receptors prominent in type II pneumocytes, making the lungs the primary initial target of infections. SARS-CoV-2 binds to the receptor-binding domain (RBD) of angiotensin-converting enzyme 2 (ACE2) (<xref ref-type="bibr" rid="B12">12</xref>); and although alveolar macrophages are recognized as the primary host entry point for Mtb (<xref ref-type="bibr" rid="B13">13</xref>, <xref ref-type="bibr" rid="B14">14</xref>), the pathogen also targets type II pneumocytes (<xref ref-type="bibr" rid="B15">15</xref>). Another similarity is the activation of Type I interferons (IFNs). The role of IFNs in driving TB pathogenesis is well-described (<xref ref-type="bibr" rid="B16">16</xref>&#x02013;<xref ref-type="bibr" rid="B18">18</xref>). Likewise, IFNs stimulate the expression of SARS-CoV-2 entry receptors and mediate endocytosis of the virus (<xref ref-type="bibr" rid="B19">19</xref>). Given the strong evidence of the involvement of IFNs in the pathogenesis of both TB and COVID-19 (<xref ref-type="bibr" rid="B20">20</xref>, <xref ref-type="bibr" rid="B21">21</xref>), the impact of heightened IFNs may be detrimental to the host. Leukopenia and lymphocytopenia are hallmarks of both TB and COVID-19 with concomitant perturbation in the numbers and function of T cells, B cells, NK cells and neutrophils (<xref ref-type="bibr" rid="B22">22</xref>&#x02013;<xref ref-type="bibr" rid="B24">24</xref>). These similarities in pathogenesis suggest that there may be synergistic impact of co-infection on immunopathogenesis. As of December 2023, PubMed lists 1,830 publications on TB and COVID-19. We are aware of only two general reviews of the interaction, one published early in 2021 and the other focused on immune mechanisms (<xref ref-type="bibr" rid="B25">25</xref>, <xref ref-type="bibr" rid="B26">26</xref>). We will discuss various facets of SARS-CoV-2 and Mtb interactions subsequent outcomes in the co-infected host based on the currently available data.</p></sec>
<sec id="s2">
<title>Impact of SARS-CoV-2 on TB disease</title>
<sec>
<title>Public health/epidemiology</title>
<p>The pandemic of SARS-CoV-2 destabilized TB control programs because personnel and laboratory resources were shifted to address the COVID-19 pandemic (<xref ref-type="bibr" rid="B27">27</xref>). As a result, the diagnosis of TB was delayed. TB treatment completion was affected, as well, by reduced mobility of both patients and health care professionals. This was superimposed on a general decline in health care access and an increase in poverty (<xref ref-type="bibr" rid="B28">28</xref>). The WHO Global TB Report released in October 2022 (<xref ref-type="bibr" rid="B29">29</xref>), showed a decrease in the number of persons reported with TB from 7.1 million in 2019 to 5.8 million in 2020 (18%) and 6.4 million in 2021; there was a major global recovery in 2022 with 7.5 million reflecting a &#x0201C;back-log&#x0201D; (<xref ref-type="bibr" rid="B30">30</xref>). In fact, deaths attributed to TB were 1.4 million in 2019, 1.5 million in 2020; 1.6 million in 2021 and 1.4 million in 2022. The COVID-19 pandemic was estimated to cause 500,000 additional deaths from TB. Other consequences of the pandemic were a 17% decline in people provided treatment for drug resistant TB, a 21% decline in provision of preventive therapy. and a decrease in global spending for essential TB services. COVID-19 also was associated with increased food insecurity worldwide; the attendant malnutrition no doubt will further increase TB prevalence in countries with a high TB burden (<xref ref-type="bibr" rid="B31">31</xref>). A recent thematic scoping review addressed the global public health impact of COVID-19 on TB including discussions of mental health and stigma (<xref ref-type="bibr" rid="B32">32</xref>).</p></sec>
<sec>
<title>Clinical manifestations</title>
<p>Co-infection with TB and COVID complicates the diagnosis and management of each because of similar transmission routes, clinical symptoms and involvement of the lungs (<xref ref-type="bibr" rid="B33">33</xref>, <xref ref-type="bibr" rid="B34">34</xref>). Pulmonary disease and pathology are a hallmark of acute COVID-19 and usually takes the form of viral pneumonia and adult respiratory distress syndrome (ARDS). Patients with COVID-19 are at risk for additional lung damage due to subsequent infections with other respiratory pathogens including TB. SARS-CoV-2 infections also are associated with hyperinflammation and multi-system inflammatory syndrome. Theoretically, there could be a failure to modulate the inflammatory response in TB co-infection with more extensive lung involvement and damage. COVID-19 patients with hyperinflammation may be treated with immunosuppressive treatment such as dexamethasone and Tocilizumab (monoclonal antibody against IL-6 receptor) which may in turn promote progression from Mtb infection to disease. In addition to TB prevalence these immunosuppressive therapies may well-impact TB presentation (late-stage presentation/more severe disease), treatment outcomes: i.e., increased mortality and treatment failure, and TB sequelae: i.e., more post TB pulmonary dysfunction. In the first published report from the Global Tuberculosis Network of 49 patients with TB and COVID-19 (<xref ref-type="bibr" rid="B35">35</xref>), 43 (88%) were symptomatic and hospitalized, 20 needed oxygen supplementation and six died. In this cohort (<xref ref-type="bibr" rid="B35">35</xref>), 26 (53%) had TB diagnosed before COVID-19, seven of whom had post-TB pulmonary sequelae. Fourteen cases (28.5%) had COVID-19 first and nine (18.3%) had both diseases diagnosed within the same week (<xref ref-type="bibr" rid="B35">35</xref>). Pulmonary, compared to extra pulmonary TB was predominant in co-infected cases as in TB alone; both drug-susceptible and drug-resistant mycobacterial strains were reported equally (<xref ref-type="bibr" rid="B35">35</xref>). Two cohort studies indicated that co-infection was more common in migrants and in males (<xref ref-type="bibr" rid="B35">35</xref>, <xref ref-type="bibr" rid="B36">36</xref>). Among patients with TB and COVID-19 both unilateral pulmonary and bilateral infiltrates have been reported (<xref ref-type="bibr" rid="B35">35</xref>, <xref ref-type="bibr" rid="B36">36</xref>). A meta-analysis of 20 studies, with a total of 205,702 patients found that patients with TB had an increased risk of mortality during a co-infection with SARS-CoV-2 (<xref ref-type="bibr" rid="B37">37</xref>). This may be explained, in part, by the reduced frequency of Mtb-specific CD4 T cells in COVID-19 patients (<xref ref-type="bibr" rid="B38">38</xref>).</p></sec>
<sec>
<title>Treatment outcomes</title>
<p>COVID-19 had a moderate effect on the prognosis and clinical response to antituberculosis treatment (ATT) in the short term (<xref ref-type="bibr" rid="B39">39</xref>). In a systematic review that examined the impact of COVID-19 co-infection with TB there was, however, no significant association between SARS-CoV-2 coinfection and un-favorable TB treatment outcomes (<xref ref-type="bibr" rid="B40">40</xref>). Completion of the long-term outcome evaluations of the global TB and COVID-19 co-infection cohort of 767 patients found that death was the outcome in &#x0003E;10% of the coinfected patients (<xref ref-type="bibr" rid="B41">41</xref>). Furthermore, among survivors, success of TB-treatment was reduced (<xref ref-type="bibr" rid="B41">41</xref>). A retrospective cohort study conducted in New York City reported significantly higher mortality risk over a 2-year period among those diagnosed with TB/COVID-19 within 120 days of each other compared to those diagnosed with TB alone (adj. HR 2.69, 95% CI: 1.66&#x02013;4.13) (<xref ref-type="bibr" rid="B42">42</xref>). Age-adjusted mortality rates were higher among California residents diagnosed with TB/COVID-19 (74.2 per 1,000 persons) compared to those diagnosed with TB during the pre-pandemic period (56.3 per 1,000 persons) (<xref ref-type="bibr" rid="B43">43</xref>). An important finding in the global co-infection cohort was that greater numbers of COVID-19 cases did not recover if SARS-CoV-2 was acquired after the end of TB treatment (26.8%) than in those with COVID-19 diagnosis before or during TB treatment (10.3%) (<xref ref-type="bibr" rid="B41">41</xref>, <xref ref-type="bibr" rid="B44">44</xref>). This suggests that post-TB lung disease negatively impacts COVID-19 disease outcome.</p></sec></sec>
<sec id="s3">
<title>Impact of SARS-CoV-2 on MTBI</title>
<p>COVID-19 may promote reactivation of TB by triggering type 1 IFN signaling which is permissive for mycobacterial growth (<xref ref-type="bibr" rid="B45">45</xref>) and through local effects such as lung inflammation and fibrosis (<xref ref-type="bibr" rid="B46">46</xref>, <xref ref-type="bibr" rid="B47">47</xref>). Immunosuppression caused by SARS-CoV-2 infection could lead to activation and progression of existing TB foci (<xref ref-type="bibr" rid="B33">33</xref>) and progression from MTBI to TB. There is a decrease in the numbers as well as functional exhaustion of T cells in response to COVID-19 infection (<xref ref-type="bibr" rid="B48">48</xref>) and COVID-19-induced cytokine storm (<xref ref-type="bibr" rid="B49">49</xref>). Rajamanickam et al. (<xref ref-type="bibr" rid="B50">50</xref>), in fact, found increased baseline and Mtb antigen induced cytokine responses in persons with MTBI in the presence of SARS-CoV-2 seropositivity. Despite biologic plausibility there are no direct data concerning how often SARS-CoV-2 leads to TB. In addition to direct effects of SARS-CoV-2 infection, corticosteroids administered to treat COVID-19 patients creates an immunosuppressive state and an opportunity for reactivation of latent TB (<xref ref-type="bibr" rid="B51">51</xref>, <xref ref-type="bibr" rid="B52">52</xref>). Treatment of COVID-19 may attenuate inflammatory processes required for host containment of MTBI (<xref ref-type="bibr" rid="B1">1</xref>, <xref ref-type="bibr" rid="B53">53</xref>). Case reports have described activation of latent TB to active TB and development of TB in patients with no history of exposure to TB during their post-COVID period (<xref ref-type="bibr" rid="B54">54</xref>, <xref ref-type="bibr" rid="B55">55</xref>), as seen in past viral pandemics (<xref ref-type="bibr" rid="B56">56</xref>). In one instance, a 40 year old female with possible latent TB developed active tuberculosis 7 weeks after her initial infection with COVID-19 (<xref ref-type="bibr" rid="B57">57</xref>). In another report, a 29-year-old healthy male with no prior exposure or history to Mtb, was diagnosed with miliary pulmonary TB after COVID-19 infection. The prolonged usage of corticosteroids for the treatment of COVID-19 can lead to reactivation of TB and also reduces the permeability of anti-TB drugs into lung epithelium impacting their effectiveness (<xref ref-type="bibr" rid="B58">58</xref>).</p></sec>
<sec id="s4">
<title>Impact of TB on SARS-CoV-2</title>
<p>In the global cohort described above of 767 TB/COVID-19 co-infected patients from 34 countries covering the period from March 2020 to June 2021, the mortality rate was 11.1% and hospitalization due to COVID-19 was 61.7% (<xref ref-type="bibr" rid="B59">59</xref>). The authors suggest that TB, either active or inactive, is an important risk factor for the development of more severe forms of COVID-19 disease. In an updated meta-analysis that included 36 studies and 60,103 COVID-19 patients from Asia, Africa, Brazil and USA, covering the period of January 2020 to May 2021, the authors found an increased risk for severe COVID-19 infection (OR = 1.56, 95% CI: 1.13&#x02013;2.16) and death (OR = 1.94, 95% CI: 1.28&#x02013;2.93) among patients with TB compared to those without TB (<xref ref-type="bibr" rid="B60">60</xref>). Therefore, TB is a significant risk factor that increases morbidity and mortality among COVID-19 patients.</p>
<p>Although experimental data on the immunopathology of TB/COVID-19 co-infection are limited, TB (past or current) induces lung damage by the proinflammatory response in the lung parenchyma that, in turn, may increase susceptibility to other airborne pathogens, such as SARS-CoV-2. The immune responses implicated in TB and COVID-19 pathogenesis are similar, involving local expression of TNF and IFN&#x003B3;, cytokines which contribute to inflammation and accumulation of active cells in the lung. During TB and COVID-19 co-infection, inflammatory stimuli may add up, leading to further lung tissue damage (<xref ref-type="bibr" rid="B1">1</xref>, <xref ref-type="bibr" rid="B61">61</xref>). A systemic manifestation of TB/COVID-19 co-infection is T-cell exhaustion. The Th-1 immune response intensified by SARS-CoV-2 infection in patients with pre-existing TB may cause depletion and dysfunction of T-cells (CD4&#x0002B; and CD8&#x0002B;), and immune dysregulation increased expression of pro-inflammatory cytokines and cytokine storm (<xref ref-type="bibr" rid="B62">62</xref>) potentially acute disease and long-term sequelae. Another mechanism by which TB may increase the susceptibility to and severity of COVID-19 is through activation of myeloid-derived suppressor cells (MDS), the known suppressors of T cells in viral infections (<xref ref-type="bibr" rid="B25">25</xref>). Further, in cases of TB with cavitary lesions, the distorted pulmonary architecture could result in increased susceptibility to SARS-CoV-2-induced pneumonia and respiratory failure. As a consequence, patients with co-infection have severe disease and poorer prognosis (<xref ref-type="bibr" rid="B1">1</xref>).</p></sec>
<sec id="s5">
<title>Impact of MTBI on SARS-CoV-2</title>
<p>MTBI may itself be associated with immune activation (<xref ref-type="bibr" rid="B63">63</xref>&#x02013;<xref ref-type="bibr" rid="B65">65</xref>) providing a potential mechanism for co-pathogenesis, and, further, inflammation may be prolonged following an episode of TB despite apparent clinical &#x0201C;cure&#x0201D; (<xref ref-type="bibr" rid="B66">66</xref>). Regarding the relationship between latent TB infection (LTBI) and COVID-19, a study which used observational case-control design involving 36 TB/COVID-19 patients from multiple primary care hospitals in China reported that COVID-1 was twice as likely to occur in those with latent TB infection (LTBI)&#x0002B;, determined through interferon-gamma release assay (IGRA), compared to the general population (<xref ref-type="bibr" rid="B61">61</xref>). This finding suggests that LTBI was a risk factor for susceptibility to COVID-19. There also was a significantly higher proportion of LTBI among severe and critical COVID-19 cases compared to those with mild and moderate disease (78 vs. 22%; <italic>p</italic> = 0.0049). Studies from the Philippines and the Global Tuberculosis Network also suggested a potential increase in susceptibility to SARS CoV-2 and increase in COVID-19 severity among patients with active or past TB or LTBI (<xref ref-type="bibr" rid="B35">35</xref>, <xref ref-type="bibr" rid="B67">67</xref>). These cohort studies however lacked adjustment for potential confounders such as comorbidities.</p>
<p>There is a theoretic possibility, in fact, that LTBI may protect against or ameliorate SARS-CoV-2 infection through trained immunity. Trained immunity is defined as epigenetic and metabolic reprogramming of innate immune cells leading to enhanced non-specific antimicrobial response to a secondary infection (<xref ref-type="bibr" rid="B68">68</xref>, <xref ref-type="bibr" rid="B69">69</xref>). This phenomenon as manifest in monocytes of individuals recently exposed to Mtb (<xref ref-type="bibr" rid="B70">70</xref>) conceivably would lead to heterologous protection and protection against or a milder course of SARS-CoV-2 infection. In a study conducted in India, severely ill patients with COVID-19 were less like to be IGRA&#x0002B; although it was not possible to discern whether IGRA&#x0002B; status was protective or severely ill patients lost their IGRA response (<xref ref-type="bibr" rid="B71">71</xref>). In a study of 76 patients with COVID-19 in Turkey a positive tuberculin skin test (TST) was associated with milder disease (<xref ref-type="bibr" rid="B72">72</xref>); again it is not clear whether COVID-19 suppressed the TST response. These results are contradictory to those reported from China and the Philippines as discussed above and raise the possibility that LTBI under certain circumstances may compromise the induction of trained immunity. In this regard, a study performed in the mouse model showed that Mtb indeed impaired the development of protective trained immunity by uniquely reprogramming haematopoietic stem cells (HSCs) via type I interferon (<xref ref-type="bibr" rid="B73">73</xref>). Despite its ability to repress trained immunity, Mtb infection induced resistance to secondary infection with SARS-CoV-2 in two different mouse models, (K18-hACE2 (ACE2) mice infected with SARS-CoV-2 and C57BL/6 mice infected with a mouse-adapted SARS-CoV-2) (<xref ref-type="bibr" rid="B74">74</xref>). Clearly, additional studies are required to fully comprehend the impact of LTBI induced host immune response on SARS-CoV-2 infection.</p></sec>
<sec id="s6">
<title>Bacillus Calmette-Gu&#x000E9;rin and heterologous protection against COVID-19</title>
<p>There is evidence, as well, that BCG vaccination of infants protects them from pathogens other than Mtb resulting in heterologous protection (<xref ref-type="bibr" rid="B75">75</xref>). Subsequent studies showed that vaccine-induced heterologous immunity was mediated by durable epigenetic modifications to the innate immune response, ergo, trained immunity (<xref ref-type="bibr" rid="B76">76</xref>, <xref ref-type="bibr" rid="B77">77</xref>). In BCG-vaccinated adults, circulating monocytes and NK cells acquired a trained phenotype, characterized by increased production of proinflammatory cytokines (<xref ref-type="bibr" rid="B68">68</xref>, <xref ref-type="bibr" rid="B69">69</xref>). Members of the IL-1 family play an important role in trained immunity (<xref ref-type="bibr" rid="B78">78</xref>). Importantly, a randomized placebo-controlled human challenge study showed that BCG-induced trained immunity provided protection <italic>in vivo</italic> in a pathogen agnostic manner (<xref ref-type="bibr" rid="B79">79</xref>). Subjects were challenged with attenuated yellow fever virus vaccine strain and the BCG-vaccinated showed lower viremia compared to controls. The decreased viremia strongly correlated with genome-wide epigenetic reprogramming of human monocytes and associated increased IL-1&#x003B2; production (<xref ref-type="bibr" rid="B79">79</xref>). Mechanistically, BCG induces trained immunity by reprogramming haematopoietic stem cells (HSCs) in the bone marrow via a type II interferon pathway (<xref ref-type="bibr" rid="B80">80</xref>).</p>
<p>These findings provided the rationale for studies of whether BCG-induced trained immunity could serve as a tool against COVID-19 (<xref ref-type="bibr" rid="B81">81</xref>, <xref ref-type="bibr" rid="B82">82</xref>). Early in the COVID-19 pandemic, several observational studies and small clinical trials suggested that trained immunity induced by BCG vaccination might have a protective effect against SARS-CoV-2 infection. For example, in a study from Turkey the decreased mortality in health care workers with COVID-19 might have been due to increased Mtb exposure history and BCG vaccination (<xref ref-type="bibr" rid="B83">83</xref>). In a unicentric, randomized-controlled clinical trial, revaccination of health care workers with BCG Moscow was associated with a lower incidence of COVID-19 positivity, though the results did not reach statistical significance (<xref ref-type="bibr" rid="B84">84</xref>). Prior BCG vaccination of health-care workers was associated with decreased SARS-CoV-2 IgG seroconversion (<xref ref-type="bibr" rid="B85">85</xref>).</p>
<p>Sixty-five randomized controlled trials of BCG to prevent or ameliorate COVID-19 have been registered to date. In a phase 3, randomized, double-blind, multicenter clinical trial in healthy elderly volunteers, vaccination with VPM1002, a genetically modified BCG, prevented severe respiratory disease including that due to COVID-19 (<xref ref-type="bibr" rid="B86">86</xref>).</p>
<p>However, data from recent clinical trials fail to show a protective role for BCG against SARS-CoV-2 infection. Meta-analyses of 7,963 participants from nine randomized controlled trials as of November 2022, showed no efficacy of BCG against COVID-19 infection (OR, 0.96; 95% CI: 0.82&#x02013;1.13); COVID-19 related-hospitalization (OR, 0.66; 95% CI: 0.37&#x02013;1.18); ICU-admission (OR, 0.25; 95% CI: 0.05&#x02013;1.18) or mortality (OR, 0.64; 95% CI: 0.17&#x02013;2.44) (<xref ref-type="bibr" rid="B87">87</xref>). Two, more recent, randomized, placebo-controlled trials in healthcare workers (BCG-Corona Study Group and BRACE Trial Consortium Group) showed that BCG vaccination did not reduce SARS-CoV-2 infections, symptomatic COVID-19 or severe COVID-19 in individuals with sero-confirmed infections as well as self-reported positive SARS-CoV-2 tests (<xref ref-type="bibr" rid="B88">88</xref>, <xref ref-type="bibr" rid="B89">89</xref>). Further, BCG vaccination, despite enhancing cytokine and antibody responses to SARS-CoV-2, had no effect on the incidence of SARS-CoV-2 infection in older adult volunteers (<xref ref-type="bibr" rid="B90">90</xref>). Nor did BCG protect health-care workers in South Africa from SARS-CoV-2 infection or related severe COVID-19 disease and hospitalization (<xref ref-type="bibr" rid="B91">91</xref>). During the COVID-19 pandemic, BCG-vaccination of HCW exposed to COVID-19 patients did not reduce unplanned absenteeism nor documented COVID-19 (<xref ref-type="bibr" rid="B92">92</xref>). In a multi-center, randomized, double-blind, placebo-controlled study on a group of 695 health care workers aged 25 years and over in Poland, statistical analysis did not reveal any significant correlation between the frequency of incidents suspected of COVID-19 and BCG-10 vaccination, the result of the tuberculin test or the number of BCG scars (<xref ref-type="bibr" rid="B93">93</xref>).</p>
<p>There seems to be a disconnect between the early observational studies and experimental models and the results of randomized clinical trials. Possible explanations are: (i) inclusion of trial participants with active TB and LTBI; (ii) variance in protective efficacy and immunogenicity across BCG strains; (iii) age and gender driven differential non-specific effects of BCG; and (iv) impact of recent vaccination history (<xref ref-type="bibr" rid="B94">94</xref>, <xref ref-type="bibr" rid="B95">95</xref>). An important factor to be considered is whether BCG was administered at birth so that the trial dose represented a vaccine boost; and also, that efficacy may vary against individual SARS-CoV-2 strains. BCG vaccination could, in fact, be effective in certain populations. In a high-risk population, a multi-center quadruple-blind study showed that BCG had protective effect and reduced the incidence of acute respiratory illness in symptomatic COVID-19 infection and the severity of the disease (<xref ref-type="bibr" rid="B96">96</xref>). In a randomized, double-blinded, placebo-controlled phase 2/3 trial conduced in the USA, the safety and efficacy of a multi-dose (BCG) vaccine for the prevention of COVID-19 was evaluated in a COVID-19-unvaccinated population of adults with type 1 diabetes. The study found that BCG vaccinated group had fewer cases of confirmed COVID-19 in comparison to the placebo group, with vaccine efficacy at 92% (<xref ref-type="bibr" rid="B97">97</xref>). The severity of COVID-19 was also significantly lower in the vaccinated group compared to the placebo. It is of interest that blood immunoglobulin G (IgG) levels specific to SARS-CoV-2 were higher in the BCG group, albeit without statistical significance (<xref ref-type="bibr" rid="B98">98</xref>). BCG also could have an immunomodulatory effect potentially increasing the efficacy of specific vaccines or prior SARS-CoV-2 infection.</p>
<p>In experimental models the efficacy of BCG against MTB is greater when administered intravenously (<xref ref-type="bibr" rid="B80">80</xref>, <xref ref-type="bibr" rid="B99">99</xref>, <xref ref-type="bibr" rid="B100">100</xref>). In mice, intravenous BCG immunization provided significant cross-protection against subsequent influenza A virus infection through the induction of trained immunity mediated by IFN&#x003B3; secreted from CX3CR1<sup>hi</sup> effector memory T cells (<xref ref-type="bibr" rid="B101">101</xref>). Intravenous BCG vaccination also elicited strong protection against SARS-CoV-2. For example, in the golden Syrian hamster model, intravenous BCG vaccination reduced viral load and SARS-CoV-2 severity (<xref ref-type="bibr" rid="B102">102</xref>). Intravenous administration of BCG also protected mice against lethal SARS-CoV-2 challenge (<xref ref-type="bibr" rid="B103">103</xref>, <xref ref-type="bibr" rid="B104">104</xref>) via BCG-specific Th1 cell mediated prolonged innate antiviral resistance (<xref ref-type="bibr" rid="B104">104</xref>). Although there are unresolved issues, the impetus to use BCG vaccine for non-specific protective effects should be pursued, especially given that the iv approach can be used with the development of &#x0201C;suicide&#x0201D; BCG strains that are cleared in immunocompetent and immunocompromised hosts (<xref ref-type="bibr" rid="B105">105</xref>).</p></sec>
<sec sec-type="conclusions" id="s7">
<title>Conclusions</title>
<p>The available data as reviewed support synergism in the co-pathogenesis of TB and COVID-19 in dually infected persons (<xref ref-type="fig" rid="F1">Figure 1</xref>). There are, however, several areas in which contradictory results have been published. The complexity of obtaining clearcut results is explained by considering that the natural history of infection with MTB and of SARS-CoV-2 each represents a continuum so that there is marked heterogeneity in the stage of co-infection at the individual level. There too are compounding effects of host demographics and co-morbidities, the infecting strain of SARS-CoV-2 and of Mtb and the level of protection conferred by prior COVID-19, vaccination and both. This leaves the possibility that several areas of uncertainty may never be resolved.</p>
<fig id="F1" position="float">
<label>Figure 1</label>
<caption><p>Schematic representation of lung pathology in <bold>(A)</bold> COVID-19 disease; <bold>(B)</bold> Mtb infected individual (MTBI) coinfected with SARS-CoV-2; <bold>(C)</bold> Individual with TB disease and SARS-CoV-2 coinfection; <bold>(D)</bold> SARS-CoV-2 infection in individual post-TB disease.</p></caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="ftubr-02-1378068-g0001.tif"/>
</fig>
<p>The impact of the COVID-19 pandemic on TB control measures and consequently on epidemiology and lethality of TB is clear-cut and well-documented. Also, the lung damage of both diseases is at least additive and may impact on short- and long-term outcomes including survival. The mechanism of co-pathogenesis appears to involve modulation of ACE on pneumocytes, Type 1 interferons, immune activation, inflammation and the cytokine storm. Although there is no evidence to date that SARS-CoV-2 vaccinations affects the incidence or prevalence of TB, it may modify co-pathogenesis.</p>
<p>Perhaps the area of greatest uncertainty is whether MTBI or revaccination with BCG confers heterologous protection against SARS-CoV-2 (<xref ref-type="fig" rid="F2">Figure 2</xref>). The evidence from <italic>ex vivo</italic> and experimental studies indicates that BCG vaccination confers training of the immune response and/or protection from heterologous challenge. The results of randomized controlled trials, however, have not shown that BCG revaccination impacts on the incidence or severity of COVID-19. The myriad of potential confounders is difficult to address. There may, however, be greater efficacy of BCG vaccination in subpopulations (high-risk groups, pre-existing Type 1 diabetes mellitus. It will be of interest to determine whether BCG modifies the immune response to other vaccines including SAR-CoV-2 vaccines. These unresolved issues assume additional importance as revaccination with BCG is under investigation for prevention of TB. The possibility that this approach will provide some level of non-specific protection against current and future pathogens is intriguing. Hopefully data from the trials that are completed or in progress will shed light on this issue.</p>
<fig id="F2" position="float">
<label>Figure 2</label>
<caption><p>Schematic representation of the varied outcome of SARS-CoV-2 following BCG vaccination.</p></caption>
<graphic mimetype="image" mime-subtype="tiff" xlink:href="ftubr-02-1378068-g0002.tif"/>
</fig></sec>
<sec sec-type="author-contributions" id="s8">
<title>Author contributions</title>
<p>PS: Conceptualization, Funding acquisition, Writing &#x02013; review &#x00026; editing. SRP: Writing &#x02013; original draft. JM: Writing &#x02013; original draft. PN: Writing &#x02013; original draft. SV: Writing &#x02013; original draft. SP: Writing &#x02013; original draft. VS: Writing &#x02013; original draft. SS: Funding acquisition, Writing &#x02013; original draft. MA: Funding acquisition, Writing &#x02013; original draft. JE: Conceptualization, Funding acquisition, Writing &#x02013; review &#x00026; editing.</p></sec>
</body>
<back>
<sec sec-type="funding-information" id="s9">
<title>Funding</title>
<p>The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by G-202105-67806 (NIAID/CRDF Global).</p>
</sec>
<sec sec-type="COI-statement" id="conf1">
<title>Conflict of interest</title>
<p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
<sec sec-type="disclaimer" id="s10">
<title>Publisher&#x00027;s note</title>
<p>All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>

<ref-list>
<title>References</title>
<ref id="B1">
<label>1.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Mousquer</surname> <given-names>GT</given-names></name> <name><surname>Peres</surname> <given-names>A</given-names></name> <name><surname>Fiegenbaum</surname> <given-names>M</given-names></name></person-group>. <article-title>Pathology of TB/COVID-19 co-infection: the phantom menace</article-title>. <source>Tuberculosis.</source> (<year>2021</year>) <volume>126</volume>:<fpage>102020</fpage>. <pub-id pub-id-type="doi">10.1016/j.tube.2020.102020</pub-id><pub-id pub-id-type="pmid">33246269</pub-id></citation></ref>
<ref id="B2">
<label>2.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Wood</surname> <given-names>R</given-names></name> <name><surname>Liang</surname> <given-names>H</given-names></name> <name><surname>Wu</surname> <given-names>H</given-names></name> <name><surname>Middelkoop</surname> <given-names>K</given-names></name> <name><surname>Oni</surname> <given-names>T</given-names></name> <name><surname>Rangaka</surname> <given-names>MX</given-names></name> <etal/></person-group>. <article-title>Changing prevalence of tuberculosis infection with increasing age in high-burden townships in South Africa</article-title>. <source>Int J Tuberc Lung Dis.</source> (<year>2010</year>) <volume>14</volume>:<fpage>406</fpage>&#x02013;<lpage>12</lpage>.<pub-id pub-id-type="pmid">20202297</pub-id></citation></ref>
<ref id="B3">
<label>3.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Kizza</surname> <given-names>FN</given-names></name> <name><surname>List</surname> <given-names>J</given-names></name> <name><surname>Nkwata</surname> <given-names>AK</given-names></name> <name><surname>Okwera</surname> <given-names>A</given-names></name> <name><surname>Ezeamama</surname> <given-names>AE</given-names></name> <name><surname>Whalen</surname> <given-names>CC</given-names></name> <etal/></person-group>. <article-title>Prevalence of latent tuberculosis infection and associated risk factors in an urban African setting</article-title>. <source>BMC Infect Dis.</source> (<year>2015</year>) <volume>15</volume>:<fpage>165</fpage>. <pub-id pub-id-type="doi">10.1186/s12879-015-0904-1</pub-id><pub-id pub-id-type="pmid">25879423</pub-id></citation></ref>
<ref id="B4">
<label>4.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Sikka</surname> <given-names>K</given-names></name> <name><surname>Yogal</surname> <given-names>R</given-names></name> <name><surname>Thakar</surname> <given-names>A</given-names></name> <name><surname>Kumar</surname> <given-names>R</given-names></name> <name><surname>Chaudhary</surname> <given-names>T</given-names></name> <name><surname>Bhartiya</surname> <given-names>M</given-names></name> <etal/></person-group>. <article-title>Objective comparison of benefits derived from contralateral routing of signal hearing aid and bone conduction device in noisy surroundings in patients with single-sided deafness</article-title>. <source>J Audiol Otol.</source> (<year>2022</year>) <volume>26</volume>:<fpage>202</fpage>&#x02013;<lpage>7</lpage>. <pub-id pub-id-type="doi">10.7874/jao.2021.00682</pub-id><pub-id pub-id-type="pmid">35405064</pub-id></citation></ref>
<ref id="B5">
<label>5.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Bruchfeld</surname> <given-names>J</given-names></name> <name><surname>Correia-Neves</surname> <given-names>M</given-names></name> <name><surname>Kallenius</surname> <given-names>G</given-names></name></person-group>. <article-title>Tuberculosis and HIV coinfection</article-title>. <source>Cold Spring Harb Perspect Med</source>. (<year>2015</year>) <volume>5</volume>:<fpage>a017871</fpage>. <pub-id pub-id-type="doi">10.1101/cshperspect.a017871</pub-id></citation>
</ref>
<ref id="B6">
<label>6.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Eriki</surname> <given-names>PP</given-names></name> <name><surname>Okwera</surname> <given-names>A</given-names></name> <name><surname>Aisu</surname> <given-names>T</given-names></name> <name><surname>Morrissey</surname> <given-names>AB</given-names></name> <name><surname>Ellner</surname> <given-names>JJ</given-names></name> <name><surname>Daniel</surname> <given-names>TM</given-names></name></person-group>. <article-title>The influence of human immunodeficiency virus infection on tuberculosis in Kampala, Uganda</article-title>. <source>Am Rev Respir Dis.</source> (<year>1991</year>) <volume>143</volume>:<fpage>185</fpage>&#x02013;<lpage>7</lpage>. <pub-id pub-id-type="doi">10.1164/ajrccm/143.1.185</pub-id><pub-id pub-id-type="pmid">1986677</pub-id></citation></ref>
<ref id="B7">
<label>7.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Whalen</surname> <given-names>C</given-names></name> <name><surname>Horsburgh</surname> <given-names>CR</given-names></name> <name><surname>Hom</surname> <given-names>D</given-names></name> <name><surname>Lahart</surname> <given-names>C</given-names></name> <name><surname>Simberkoff</surname> <given-names>M</given-names></name> <name><surname>Ellner</surname> <given-names>J</given-names></name></person-group>. <article-title>Accelerated course of human immunodeficiency virus infection after tuberculosis</article-title>. <source>Am J Respir Crit Care Med.</source> (<year>1995</year>) <volume>151</volume>:<fpage>129</fpage>&#x02013;<lpage>35</lpage>. <pub-id pub-id-type="doi">10.1164/ajrccm.151.1.7812542</pub-id><pub-id pub-id-type="pmid">7812542</pub-id></citation></ref>
<ref id="B8">
<label>8.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Whalen</surname> <given-names>CC</given-names></name> <name><surname>Nsubuga</surname> <given-names>P</given-names></name> <name><surname>Okwera</surname> <given-names>A</given-names></name> <name><surname>Johnson</surname> <given-names>JL</given-names></name> <name><surname>Hom</surname> <given-names>DL</given-names></name> <name><surname>Michael</surname> <given-names>NL</given-names></name> <etal/></person-group>. <article-title>Impact of pulmonary tuberculosis on survival of HIV-infected adults: a prospective epidemiologic study in Uganda</article-title>. <source>AIDS.</source> (<year>2000</year>) <volume>14</volume>:<fpage>1219</fpage>&#x02013;<lpage>28</lpage>. <pub-id pub-id-type="doi">10.1097/00002030-200006160-00020</pub-id><pub-id pub-id-type="pmid">10894287</pub-id></citation></ref>
<ref id="B9">
<label>9.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Baluku</surname> <given-names>JB</given-names></name> <name><surname>Nassozi</surname> <given-names>S</given-names></name> <name><surname>Gyagenda</surname> <given-names>B</given-names></name> <name><surname>Namanda</surname> <given-names>M</given-names></name> <name><surname>Andia-Biraro</surname> <given-names>I</given-names></name> <name><surname>Worodria</surname> <given-names>W</given-names></name> <etal/></person-group>. <article-title>Prevalence of malaria and TB coinfection at a national tuberculosis treatment centre in Uganda</article-title>. <source>J Trop Med</source>. (<year>2019</year>) <volume>2019</volume>:<fpage>3741294</fpage>. <pub-id pub-id-type="doi">10.1155/2019/3741294</pub-id><pub-id pub-id-type="pmid">31428162</pub-id></citation></ref>
<ref id="B10">
<label>10.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Alfaraj</surname> <given-names>SH</given-names></name> <name><surname>Al-Tawfiq</surname> <given-names>JA</given-names></name> <name><surname>Altuwaijri</surname> <given-names>TA</given-names></name> <name><surname>Memish</surname> <given-names>ZA</given-names></name></person-group>. <article-title>Middle east respiratory syndrome coronavirus and pulmonary tuberculosis coinfection: implications for infection control</article-title>. <source>Intervirology.</source> (<year>2017</year>) <volume>60</volume>:<fpage>53</fpage>&#x02013;<lpage>5</lpage>. <pub-id pub-id-type="doi">10.1159/000477908</pub-id><pub-id pub-id-type="pmid">28683463</pub-id></citation></ref>
<ref id="B11">
<label>11.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Low</surname> <given-names>JG</given-names></name> <name><surname>Lee</surname> <given-names>CC</given-names></name> <name><surname>Leo</surname> <given-names>YS</given-names></name> <name><surname>Low</surname> <given-names>JG</given-names></name> <name><surname>Lee</surname> <given-names>CC</given-names></name> <name><surname>Leo</surname> <given-names>YS</given-names></name></person-group>. <article-title>Severe acute respiratory syndrome and pulmonary tuberculosis</article-title>. <source>Clin Infect Dis.</source> (<year>2004</year>) <volume>38</volume>:<fpage>e123</fpage>&#x02013;<lpage>5</lpage>. <pub-id pub-id-type="doi">10.1086/421396</pub-id><pub-id pub-id-type="pmid">15227635</pub-id></citation></ref>
<ref id="B12">
<label>12.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Hoffmann</surname> <given-names>M</given-names></name> <name><surname>Kleine-Weber</surname> <given-names>H</given-names></name> <name><surname>Schroeder</surname> <given-names>S</given-names></name> <name><surname>Kr&#x000FC;ger</surname> <given-names>N</given-names></name> <name><surname>Herrler</surname> <given-names>T</given-names></name> <name><surname>Erichsen</surname> <given-names>S</given-names></name> <etal/></person-group>. <article-title>SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor</article-title>. <source>Cell</source>. (<year>2020</year>) <volume>181</volume>:<fpage>271</fpage>. <pub-id pub-id-type="doi">10.1016/j.cell.2020.02.052</pub-id><pub-id pub-id-type="pmid">32142651</pub-id></citation></ref>
<ref id="B13">
<label>13.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Cohen</surname> <given-names>SB</given-names></name> <name><surname>Gern</surname> <given-names>BH</given-names></name> <name><surname>Delahaye</surname> <given-names>JL</given-names></name> <name><surname>Adams</surname> <given-names>KN</given-names></name> <name><surname>Plumlee</surname> <given-names>CR</given-names></name> <name><surname>Winkler</surname> <given-names>JK</given-names></name> <etal/></person-group>. <article-title>Alveolar macrophages provide an early <italic>Mycobacterium tuberculosis</italic> Niche and initiate dissemination</article-title>. <source>Cell Host Microbe</source>. (<year>2018</year>) 24:439&#x02013;46 e4. <pub-id pub-id-type="doi">10.1016/j.chom.2018.08.001</pub-id><pub-id pub-id-type="pmid">30146391</pub-id></citation></ref>
<ref id="B14">
<label>14.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Huang</surname> <given-names>L</given-names></name> <name><surname>Nazarova</surname> <given-names>EV</given-names></name> <name><surname>Tan</surname> <given-names>S</given-names></name> <name><surname>Liu</surname> <given-names>Y</given-names></name> <name><surname>Russell</surname> <given-names>DG</given-names></name></person-group>. <article-title>Growth of <italic>Mycobacterium tuberculosis in vivo</italic> segregates with host macrophage metabolism and ontogeny</article-title>. <source>J Exp Med.</source> (<year>2018</year>) <volume>215</volume>:<fpage>1135</fpage>&#x02013;<lpage>52</lpage>. <pub-id pub-id-type="doi">10.1084/jem.20172020</pub-id><pub-id pub-id-type="pmid">29500179</pub-id></citation></ref>
<ref id="B15">
<label>15.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Ganbat</surname> <given-names>D</given-names></name> <name><surname>Seehase</surname> <given-names>S</given-names></name> <name><surname>Richter</surname> <given-names>E</given-names></name> <name><surname>Vollmer</surname> <given-names>E</given-names></name> <name><surname>Reiling</surname> <given-names>N</given-names></name> <name><surname>Fellenberg</surname> <given-names>K</given-names></name> <etal/></person-group>. <article-title>Mycobacteria infect different cell types in the human lung and cause species dependent cellular changes in infected cells</article-title>. <source>BMC Pulm Med.</source> (<year>2016</year>) <volume>16</volume>:<fpage>19</fpage>. <pub-id pub-id-type="doi">10.1186/s12890-016-0185-5</pub-id><pub-id pub-id-type="pmid">26803467</pub-id></citation></ref>
<ref id="B16">
<label>16.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Mayer-Barber</surname> <given-names>KD</given-names></name> <name><surname>Andrade</surname> <given-names>BB</given-names></name> <name><surname>Oland</surname> <given-names>SD</given-names></name> <name><surname>Amaral</surname> <given-names>EP</given-names></name> <name><surname>Barber</surname> <given-names>DL</given-names></name> <name><surname>Gonzales</surname> <given-names>J</given-names></name> <etal/></person-group>. <article-title>Host-directed therapy of tuberculosis based on interleukin-1 and type I interferon crosstalk</article-title>. <source>Nature.</source> (<year>2014</year>) <volume>511</volume>:<fpage>99</fpage>&#x02013;<lpage>103</lpage>. <pub-id pub-id-type="doi">10.1038/nature13489</pub-id><pub-id pub-id-type="pmid">24990750</pub-id></citation></ref>
<ref id="B17">
<label>17.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Moreira-Teixeira</surname> <given-names>L</given-names></name> <name><surname>Mayer-Barber</surname> <given-names>K</given-names></name> <name><surname>Sher</surname> <given-names>A</given-names></name> <name><surname>O&#x00027;Garra</surname> <given-names>A</given-names></name></person-group>. <article-title>Type I interferons in tuberculosis: foe and occasionally friend</article-title>. <source>J Exp Med.</source> (<year>2018</year>) <volume>215</volume>:<fpage>1273</fpage>&#x02013;<lpage>85</lpage>. <pub-id pub-id-type="doi">10.1084/jem.20180325</pub-id><pub-id pub-id-type="pmid">29666166</pub-id></citation></ref>
<ref id="B18">
<label>18.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Moreira-Teixeira</surname> <given-names>L</given-names></name> <name><surname>Stimpson</surname> <given-names>PJ</given-names></name> <name><surname>Stavropoulos</surname> <given-names>E</given-names></name> <name><surname>Hadebe</surname> <given-names>S</given-names></name> <name><surname>Chakravarty</surname> <given-names>P</given-names></name> <name><surname>Ioannou</surname> <given-names>M</given-names></name> <etal/></person-group>. <article-title>Type I IFN exacerbates disease in tuberculosis-susceptible mice by inducing neutrophil-mediated lung inflammation and NETosis</article-title>. <source>Nat Commun.</source> (<year>2020</year>) <volume>11</volume>:<fpage>5566</fpage>. <pub-id pub-id-type="doi">10.1038/s41467-020-19412-6</pub-id><pub-id pub-id-type="pmid">33149141</pub-id></citation></ref>
<ref id="B19">
<label>19.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Yang</surname> <given-names>P</given-names></name> <name><surname>Wang</surname> <given-names>X</given-names></name></person-group>. <article-title>COVID-19: a new challenge for human beings</article-title>. <source>Cell Mol Immunol.</source> (<year>2020</year>) <volume>17</volume>:<fpage>555</fpage>&#x02013;<lpage>7</lpage>. <pub-id pub-id-type="doi">10.1038/s41423-020-0407-x</pub-id><pub-id pub-id-type="pmid">32235915</pub-id></citation></ref>
<ref id="B20">
<label>20.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Masood</surname> <given-names>KI</given-names></name> <name><surname>Yameen</surname> <given-names>M</given-names></name> <name><surname>Ashraf</surname> <given-names>J</given-names></name> <name><surname>Shahid</surname> <given-names>S</given-names></name> <name><surname>Mahmood</surname> <given-names>SF</given-names></name> <name><surname>Nasir</surname> <given-names>A</given-names></name> <etal/></person-group>. <article-title>Upregulated type I interferon responses in asymptomatic COVID-19 infection are associated with improved clinical outcome</article-title>. <source>Sci Rep.</source> (<year>2021</year>) <volume>11</volume>:<fpage>22958</fpage>. <pub-id pub-id-type="doi">10.1038/s41598-021-02489-4</pub-id><pub-id pub-id-type="pmid">34824360</pub-id></citation></ref>
<ref id="B21">
<label>21.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Cliff</surname> <given-names>JM</given-names></name> <name><surname>Kaufmann</surname> <given-names>SH</given-names></name> <name><surname>McShane</surname> <given-names>H</given-names></name> <name><surname>van Helden</surname> <given-names>P</given-names></name> <name><surname>O&#x00027;Garra</surname> <given-names>A</given-names></name></person-group>. <article-title>The human immune response to tuberculosis and its treatment: a view from the blood</article-title>. <source>Immunol Rev.</source> (<year>2015</year>) <volume>264</volume>:<fpage>88</fpage>&#x02013;<lpage>102</lpage>. <pub-id pub-id-type="doi">10.1111/imr.12269</pub-id><pub-id pub-id-type="pmid">25703554</pub-id></citation></ref>
<ref id="B22">
<label>22.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Musso</surname> <given-names>M</given-names></name> <name><surname>Di Gennaro</surname> <given-names>F</given-names></name> <name><surname>Gualano</surname> <given-names>G</given-names></name> <name><surname>Mosti</surname> <given-names>S</given-names></name> <name><surname>Cerva</surname> <given-names>C</given-names></name> <name><surname>Fard</surname> <given-names>SN</given-names></name> <etal/></person-group>. <article-title>Concurrent cavitary pulmonary tuberculosis and COVID-19 pneumonia with <italic>in vitro</italic> immune cell anergy</article-title>. <source>Infection.</source> (<year>2021</year>) <volume>49</volume>:<fpage>1061</fpage>&#x02013;<lpage>4</lpage>. <pub-id pub-id-type="doi">10.1007/s15010-021-01576-y</pub-id><pub-id pub-id-type="pmid">33454928</pub-id></citation></ref>
<ref id="B23">
<label>23.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Luo</surname> <given-names>Y</given-names></name> <name><surname>Xie</surname> <given-names>Y</given-names></name> <name><surname>Zhang</surname> <given-names>W</given-names></name> <name><surname>Lin</surname> <given-names>Q</given-names></name> <name><surname>Tang</surname> <given-names>G</given-names></name> <name><surname>Wu</surname> <given-names>S</given-names></name> <etal/></person-group>. <article-title>Combination of lymphocyte number and function in evaluating host immunit.y Aging</article-title>. (<year>2019</year>) <volume>11</volume>:<fpage>12685</fpage>&#x02013;<lpage>707</lpage>. <pub-id pub-id-type="doi">10.18632/aging.102595</pub-id><pub-id pub-id-type="pmid">31857499</pub-id></citation></ref>
<ref id="B24">
<label>24.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Muefong</surname> <given-names>CN</given-names></name> <name><surname>Sutherland</surname> <given-names>JS</given-names></name></person-group>. <article-title>Neutrophils in tuberculosis-associated inflammation and lung pathology</article-title>. <source>Front Immunol.</source> (<year>2020</year>) <volume>11</volume>:<fpage>962</fpage>. <pub-id pub-id-type="doi">10.3389/fimmu.2020.00962</pub-id><pub-id pub-id-type="pmid">32536917</pub-id></citation></ref>
<ref id="B25">
<label>25.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Visca</surname> <given-names>D</given-names></name> <name><surname>Ong</surname> <given-names>CWM</given-names></name> <name><surname>Tiberi</surname> <given-names>S</given-names></name> <name><surname>Centis</surname> <given-names>R</given-names></name> <name><surname>D&#x00027;Ambrosio</surname> <given-names>L</given-names></name> <name><surname>Chen</surname> <given-names>B</given-names></name> <etal/></person-group>. <article-title>Tuberculosis and COVID-19 interaction: a review of biological, clinical and public health effects</article-title>. <source>Pulmonology.</source> (<year>2021</year>) <volume>27</volume>:<fpage>151</fpage>&#x02013;<lpage>65</lpage>. <pub-id pub-id-type="doi">10.1016/j.pulmoe.2020.12.012</pub-id><pub-id pub-id-type="pmid">33547029</pub-id></citation></ref>
<ref id="B26">
<label>26.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Booysen</surname> <given-names>P</given-names></name> <name><surname>Wilkinson</surname> <given-names>KA</given-names></name> <name><surname>Sheerin</surname> <given-names>D</given-names></name> <name><surname>Waters</surname> <given-names>R</given-names></name> <name><surname>Coussens</surname> <given-names>AK</given-names></name> <name><surname>Wilkinson</surname> <given-names>RJ</given-names></name> <etal/></person-group>. <article-title>Immune interaction between SARS-CoV-2 and Mycobacterium tuberculosis</article-title>. <source>Front Immunol.</source> (<year>2023</year>) <volume>14</volume>:<fpage>1254206</fpage>. <pub-id pub-id-type="doi">10.3389/fimmu.2023.1254206</pub-id><pub-id pub-id-type="pmid">37841282</pub-id></citation></ref>
<ref id="B27">
<label>27.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Pai</surname> <given-names>M</given-names></name> <name><surname>Kasaeva</surname> <given-names>T</given-names></name> <name><surname>Swaminathan</surname> <given-names>S</given-names></name></person-group>. <article-title>Covid-19&#x00027;s devastating effect on tuberculosis care - a path to recovery</article-title>. <source>N Engl J Med.</source> (<year>2022</year>) <volume>386</volume>:<fpage>1490</fpage>&#x02013;<lpage>3</lpage>. <pub-id pub-id-type="doi">10.1056/NEJMp2118145</pub-id><pub-id pub-id-type="pmid">34986295</pub-id></citation></ref>
<ref id="B28">
<label>28.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>McQuaid</surname> <given-names>CF</given-names></name> <name><surname>Vassall</surname> <given-names>A</given-names></name> <name><surname>Cohen</surname> <given-names>T</given-names></name> <name><surname>Fiekert</surname> <given-names>K</given-names></name> <name><surname>White</surname> <given-names>RG</given-names></name></person-group>. <article-title>The impact of COVID-19 on TB: a review of the data</article-title>. <source>Int J Tuberc Lung Dis.</source> (<year>2021</year>) <volume>25</volume>:<fpage>436</fpage>&#x02013;<lpage>46</lpage>. <pub-id pub-id-type="doi">10.5588/ijtld.21.0148</pub-id></citation>
</ref>
<ref id="B29">
<label>29.</label>
<citation citation-type="book"><person-group person-group-type="author"><collab>WHO</collab></person-group>. <source>Global Tuberculosis Report 2022</source>. <publisher-loc>Geneva</publisher-loc>: <publisher-name>World Health Organization</publisher-name> (<year>2022</year>).</citation>
</ref>
<ref id="B30">
<label>30.</label>
<citation citation-type="book"><person-group person-group-type="author"><collab>WHO</collab></person-group>. <source>Report, G.t., Licence: CC BY-NC-SA 3.0 IGO</source>. <publisher-loc>Geneva</publisher-loc>: <publisher-name>World Health Organization</publisher-name> (<year>2023</year>).</citation>
</ref>
<ref id="B31">
<label>31.</label>
<citation citation-type="journal"><person-group person-group-type="author"><collab>Carwile ME Hochberg NS Sinha P Undernutrition is feeding the tuberculosis pandemic: a perspective</collab></person-group>. <source>J Clin Tuberc Other Mycobact Dis</source>. (<year>2022</year>) <volume>27</volume>:<fpage>100311</fpage>. <pub-id pub-id-type="doi">10.1016/j.jctube.2022.100311</pub-id></citation>
</ref>
<ref id="B32">
<label>32.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Marco</surname> <given-names>MH</given-names></name> <name><surname>Ahmedov</surname> <given-names>S</given-names></name> <name><surname>Castro</surname> <given-names>KG</given-names></name></person-group>. <article-title>The global impact of COVID-19 on tuberculosis: a thematic scoping review, 2020-2023</article-title>. <source>medRxiv.</source> (<year>2024</year>). <pub-id pub-id-type="doi">10.1101/2024.03.04.24303743</pub-id></citation>
</ref>
<ref id="B33">
<label>33.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Lin</surname> <given-names>L</given-names></name> <name><surname>Lu</surname> <given-names>L</given-names></name> <name><surname>Cao</surname> <given-names>W</given-names></name> <name><surname>Li</surname> <given-names>T</given-names></name></person-group>. <article-title>Hypothesis for potential pathogenesis of SARS-CoV-2 infection-a review of immune changes in patients with viral pneumonia</article-title>. <source>Emerg Microbes Infect.</source> (<year>2020</year>) <volume>9</volume>:<fpage>727</fpage>&#x02013;<lpage>32</lpage>. <pub-id pub-id-type="doi">10.1080/22221751.2020.1746199</pub-id><pub-id pub-id-type="pmid">32196410</pub-id></citation></ref>
<ref id="B34">
<label>34.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Yang</surname> <given-names>X</given-names></name> <name><surname>Yu</surname> <given-names>Y</given-names></name> <name><surname>Xu</surname> <given-names>J</given-names></name> <name><surname>Shu</surname> <given-names>H</given-names></name> <name><surname>Xia</surname> <given-names>J</given-names></name> <name><surname>Liu</surname> <given-names>H</given-names></name> <etal/></person-group>. <article-title>Clinical course and outcomes of critically ill patients with SARS-CoV-2 pneumonia in Wuhan, China: a single-centered, retrospective, observational study</article-title>. <source>Lancet Respir Med.</source> (<year>2020</year>) <volume>8</volume>:<fpage>475</fpage>&#x02013;<lpage>81</lpage>. <pub-id pub-id-type="doi">10.1016/S2213-2600(20)30079-5</pub-id><pub-id pub-id-type="pmid">32105632</pub-id></citation></ref>
<ref id="B35">
<label>35.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Tadolini</surname> <given-names>M</given-names></name> <name><surname>Codecasa</surname> <given-names>LR</given-names></name> <name><surname>Garc&#x000ED;a-Garc&#x000ED;a</surname> <given-names>JM</given-names></name> <name><surname>Blanc</surname> <given-names>FX</given-names></name> <name><surname>Borisov</surname> <given-names>S</given-names></name> <name><surname>Alffenaar</surname> <given-names>JW</given-names></name> <etal/></person-group>. <article-title>Active tuberculosis, sequelae and COVID-19 co-infection: first cohort of 49 cases</article-title>. <source>Eur Respir J</source>. (<year>2020</year>) <fpage>56</fpage>. <pub-id pub-id-type="doi">10.1183/13993003.02328-2020</pub-id><pub-id pub-id-type="pmid">32457198</pub-id></citation></ref>
<ref id="B36">
<label>36.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Motta</surname> <given-names>I</given-names></name> <name><surname>Centis</surname> <given-names>R</given-names></name> <name><surname>D&#x00027;Ambrosio</surname> <given-names>L</given-names></name> <name><surname>Garc&#x000ED;a-Garc&#x000ED;a</surname> <given-names>JM</given-names></name> <name><surname>Goletti</surname> <given-names>D</given-names></name> <name><surname>Gualano</surname> <given-names>G</given-names></name> <etal/></person-group>. <article-title>Tuberculosis, COVID-19 and migrants: preliminary analysis of deaths occurring in 69 patients from two cohorts</article-title>. <source>Pulmonology.</source> (<year>2020</year>) <volume>26</volume>:<fpage>233</fpage>&#x02013;<lpage>40</lpage>. <pub-id pub-id-type="doi">10.1016/j.pulmoe.2020.05.002</pub-id><pub-id pub-id-type="pmid">32411943</pub-id></citation></ref>
<ref id="B37">
<label>37.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Sarkar</surname> <given-names>S</given-names></name> <name><surname>Khanna</surname> <given-names>P</given-names></name> <name><surname>Singh</surname> <given-names>AK</given-names></name></person-group>. <article-title>Impact of COVID-19 in patients with concurrent co-infections: a systematic review and meta-analyses</article-title>. <source>J Med Virol.</source> (<year>2021</year>) <volume>93</volume>:<fpage>2385</fpage>&#x02013;<lpage>95</lpage>. <pub-id pub-id-type="doi">10.1002/jmv.26740</pub-id><pub-id pub-id-type="pmid">33331656</pub-id></citation></ref>
<ref id="B38">
<label>38.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Riou</surname> <given-names>C</given-names></name> <name><surname>du Bruyn</surname> <given-names>E</given-names></name> <name><surname>Stek</surname> <given-names>C</given-names></name> <name><surname>Daroowala</surname> <given-names>R</given-names></name> <name><surname>Goliath</surname> <given-names>RT</given-names></name> <name><surname>Abrahams</surname> <given-names>F</given-names></name> <etal/></person-group>. <article-title>Relationship of SARS-CoV-2-specific CD4 response to COVID-19 severity and impact of HIV-1 and tuberculosis coinfection</article-title>. <source>J Clin Invest</source>. (<year>2021</year>) 131 <pub-id pub-id-type="doi">10.1172/JCI149125</pub-id><pub-id pub-id-type="pmid">33945513</pub-id></citation></ref>
<ref id="B39">
<label>39.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Guest</surname> <given-names>PC</given-names></name></person-group>. <article-title>Clinical, biological and molecular aspects of COVID-19 preface</article-title>. <source>Clin Biol Mol Aspects Covid-19</source>. (<year>2021</year>) 1321:V <pub-id pub-id-type="doi">10.1007/978-3-030-59261-5</pub-id></citation>
</ref>
<ref id="B40">
<label>40.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Vadlamudi</surname> <given-names>NK</given-names></name> <name><surname>Basham</surname> <given-names>CA</given-names></name> <name><surname>Johnston</surname> <given-names>JC</given-names></name> <name><surname>Ahmad Khan</surname> <given-names>F</given-names></name> <name><surname>Battista Migliori</surname> <given-names>G</given-names></name> <name><surname>Centis</surname> <given-names>R</given-names></name> <etal/></person-group>. <article-title>The association of SARS-CoV-2 infection and tuberculosis disease with unfavorable treatment outcomes: a systematic review</article-title>. <source>PLOS Glob Public Health.</source> (<year>2023</year>) <volume>3</volume>:<fpage>e0002163</fpage>. <pub-id pub-id-type="doi">10.1371/journal.pgph.0002163</pub-id></citation>
</ref>
<ref id="B41">
<label>41.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Casco</surname> <given-names>N</given-names></name> <name><surname>Jorge</surname> <given-names>AL</given-names></name> <name><surname>Palmero</surname> <given-names>DJ</given-names></name> <name><surname>Alffenaar</surname> <given-names>JW</given-names></name> <name><surname>Fox</surname> <given-names>GJ</given-names></name> <name><surname>Ezz</surname> <given-names>W</given-names></name> <etal/></person-group>. <article-title>Long-term outcomes of the global tuberculosis and COVID-19 co-infection cohort</article-title>. <source>Eur Respir J</source>. (<year>2023</year>) <volume>62</volume>:<fpage>2300925</fpage>. <pub-id pub-id-type="doi">10.1183/13993003.00925-2023</pub-id><pub-id pub-id-type="pmid">37827576</pub-id></citation></ref>
<ref id="B42">
<label>42.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Easton</surname> <given-names>AV</given-names></name> <name><surname>Salerno</surname> <given-names>MM</given-names></name> <name><surname>Trieu</surname> <given-names>L</given-names></name> <name><surname>Humphrey</surname> <given-names>E</given-names></name> <name><surname>Kaba</surname> <given-names>F</given-names></name> <name><surname>Macaraig</surname> <given-names>M</given-names></name> <etal/></person-group>. <article-title>Cohort study of the mortality among patients in New York City with tuberculosis and COVID-19, March 2020 to June 2022</article-title>. <source>PLOS Glob Public Health.</source> (<year>2023</year>) <volume>3</volume>:<fpage>e0001758</fpage>. <pub-id pub-id-type="doi">10.1371/journal.pgph.0001758</pub-id><pub-id pub-id-type="pmid">37186110</pub-id></citation></ref>
<ref id="B43">
<label>43.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Nabity</surname> <given-names>SA</given-names></name> <name><surname>Han</surname> <given-names>E</given-names></name> <name><surname>Lowenthal</surname> <given-names>P</given-names></name> <name><surname>Henry</surname> <given-names>H</given-names></name> <name><surname>Okoye</surname> <given-names>N</given-names></name> <name><surname>Chakrabarty</surname> <given-names>M</given-names></name> <etal/></person-group>. <article-title>Sociodemographic characteristics, comorbidities, and mortality among persons diagnosed with tuberculosis and COVID-19 in close succession in California</article-title>. (<year>2020</year>) <italic>JAMA Netw Open</italic>. (<year>2021</year>) <volume>4</volume>:<fpage>e2136853</fpage>. <pub-id pub-id-type="doi">10.1001/jamanetworkopen.2021.36853</pub-id><pub-id pub-id-type="pmid">34860244</pub-id></citation></ref>
<ref id="B44">
<label>44.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Diel</surname> <given-names>R</given-names></name> <name><surname>Schluger</surname> <given-names>NW</given-names></name></person-group>. <article-title>Long-term outcome of co-infection of COVID-19 and tuberculosis: the &#x0201C;cursed duet&#x0201D;</article-title>. <source>Eur Respir J</source>. (<year>2023</year>) <fpage>62</fpage>. <pub-id pub-id-type="doi">10.1183/13993003.01881-2023</pub-id></citation>
</ref>
<ref id="B45">
<label>45.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Singh</surname> <given-names>A</given-names></name> <name><surname>Prasad</surname> <given-names>R</given-names></name> <name><surname>Gupta</surname> <given-names>A</given-names></name> <name><surname>Das</surname> <given-names>K</given-names></name> <name><surname>Gupta</surname> <given-names>N</given-names></name></person-group>. <article-title>Severe acute respiratory syndrome coronavirus-2 and pulmonary tuberculosis: convergence can be fatal</article-title>. <source>Monaldi Arch Chest Dis.</source> (<year>2020</year>) <volume>90</volume>:<fpage>441</fpage>&#x02013;<lpage>50</lpage>. <pub-id pub-id-type="doi">10.4081/monaldi.2020.1368</pub-id><pub-id pub-id-type="pmid">32697060</pub-id></citation></ref>
<ref id="B46">
<label>46.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Luke</surname> <given-names>E</given-names></name> <name><surname>Swafford</surname> <given-names>K</given-names></name> <name><surname>Shirazi</surname> <given-names>G</given-names></name> <name><surname>Venketaraman</surname> <given-names>V</given-names></name></person-group>. <article-title>TB and COVID-19: an exploration of the characteristics and resulting complications of co-infection</article-title>. <source>Front Biosci.</source> (<year>2022</year>) <volume>14</volume>:<fpage>6</fpage>. <pub-id pub-id-type="doi">10.31083/j.fbs1401006</pub-id><pub-id pub-id-type="pmid">35320917</pub-id></citation></ref>
<ref id="B47">
<label>47.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Elziny</surname> <given-names>MM</given-names></name> <name><surname>Ghazy</surname> <given-names>A</given-names></name> <name><surname>Elfert</surname> <given-names>KA</given-names></name> <name><surname>Aboukamar</surname> <given-names>M</given-names></name></person-group>. <article-title>Case report: development of miliary pulmonary tuberculosis in a patient with peritoneal tuberculosis after COVID-19 upper respiratory tract infection</article-title>. <source>Am J Trop Med Hyg.</source> (<year>2021</year>) <volume>104</volume>:<fpage>1792</fpage>&#x02013;<lpage>5</lpage>. <pub-id pub-id-type="doi">10.4269/ajtmh.20-1156</pub-id><pub-id pub-id-type="pmid">33784243</pub-id></citation></ref>
<ref id="B48">
<label>48.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Diao</surname> <given-names>B</given-names></name> <name><surname>Wang</surname> <given-names>C</given-names></name> <name><surname>Tan</surname> <given-names>Y</given-names></name> <name><surname>Chen</surname> <given-names>X</given-names></name> <name><surname>Liu</surname> <given-names>Y</given-names></name> <name><surname>Ning</surname> <given-names>L</given-names></name> <etal/></person-group>. <article-title>Reduction and functional exhaustion of T cells in patients with coronavirus disease 2019 (COVID-19)</article-title>. <source>Front Immunol</source>. (<year>2020</year>) <volume>11</volume>:<fpage>827</fpage>. <pub-id pub-id-type="doi">10.3389/fimmu.2020.00827</pub-id><pub-id pub-id-type="pmid">32425950</pub-id></citation></ref>
<ref id="B49">
<label>49.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Manson</surname> <given-names>JJ</given-names></name> <name><surname>Crooks</surname> <given-names>C</given-names></name> <name><surname>Naja</surname> <given-names>M</given-names></name> <name><surname>Ledlie</surname> <given-names>A</given-names></name> <name><surname>Goulden</surname> <given-names>B</given-names></name> <name><surname>Liddle</surname> <given-names>T</given-names></name> <etal/></person-group>. <article-title>COVID-19-associated hyperinflammation and escalation of patient care: a retrospective longitudinal cohort study</article-title>. <source>Lancet Rheumatol.</source> (<year>2020</year>) <volume>2</volume>:<fpage>e594</fpage>&#x02013;<lpage>602</lpage>. <pub-id pub-id-type="doi">10.1016/S2665-9913(20)30275-7</pub-id><pub-id pub-id-type="pmid">32864628</pub-id></citation></ref>
<ref id="B50">
<label>50.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Rajamanickam</surname> <given-names>A</given-names></name> <name><surname>Kumar</surname> <given-names>NP</given-names></name> <name><surname>Chandrasekaran</surname> <given-names>P</given-names></name> <name><surname>Nancy</surname> <given-names>A</given-names></name> <name><surname>Bhavani</surname> <given-names>PK</given-names></name> <name><surname>Selvaraj</surname> <given-names>N</given-names></name> <etal/></person-group>. <article-title>Effect of SARS-CoV-2 seropositivity on antigen - specific cytokine and chemokine responses in latent tuberculosis</article-title>. <source>Cytokine.</source> (<year>2022</year>) <volume>150</volume>:<fpage>155785</fpage>. <pub-id pub-id-type="doi">10.1016/j.cyto.2021.155785</pub-id><pub-id pub-id-type="pmid">34933240</pub-id></citation></ref>
<ref id="B51">
<label>51.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Gopalaswamy</surname> <given-names>R</given-names></name> <name><surname>Subbian</surname> <given-names>S</given-names></name></person-group>. <article-title>Corticosteroids for COVID-19 therapy: potential implications on tuberculosis</article-title>. <source>Int J Mol Sci</source>. (<year>2021</year>) <fpage>22</fpage>. <pub-id pub-id-type="doi">10.3390/ijms22073773</pub-id><pub-id pub-id-type="pmid">33917321</pub-id></citation></ref>
<ref id="B52">
<label>52.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Shariq</surname> <given-names>M</given-names></name> <name><surname>Sheikh</surname> <given-names>JA</given-names></name> <name><surname>Quadir</surname> <given-names>N</given-names></name> <name><surname>Sharma</surname> <given-names>N</given-names></name> <name><surname>Hasnain</surname> <given-names>SE</given-names></name> <name><surname>Ehtesham</surname> <given-names>NZ</given-names></name></person-group>. <article-title>COVID-19 and tuberculosis: the double whammy of respiratory pathogens</article-title>. <source>Eur Respir Rev</source>. (<year>2022</year>) <volume>31</volume>:<fpage>210264</fpage>. <pub-id pub-id-type="doi">10.1183/16000617.0264-2021</pub-id><pub-id pub-id-type="pmid">35418488</pub-id></citation></ref>
<ref id="B53">
<label>53.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Robert</surname> <given-names>M</given-names></name> <name><surname>Miossec</surname> <given-names>P</given-names></name></person-group>. <article-title>Reactivation of latent tuberculosis with TNF inhibitors: critical role of the beta 2 chain of the IL-12 receptor</article-title>. <source>Cell Mol Immunol.</source> (<year>2021</year>) <volume>18</volume>:<fpage>1644</fpage>&#x02013;<lpage>51</lpage>. <pub-id pub-id-type="doi">10.1038/s41423-021-00694-9</pub-id><pub-id pub-id-type="pmid">34021269</pub-id></citation></ref>
<ref id="B54">
<label>54.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Al-Kayali</surname> <given-names>RS</given-names></name> <name><surname>Kashkash</surname> <given-names>MF</given-names></name> <name><surname>Alhussein Alhajji</surname> <given-names>AH</given-names></name> <name><surname>Khouri</surname> <given-names>A</given-names></name></person-group>. <article-title>Activation of tuberculosis in recovered COVID-19 patients: a case report</article-title>. <source>Ann Med Surg.</source> (<year>2023</year>) <volume>85</volume>:<fpage>280</fpage>&#x02013;<lpage>3</lpage>. <pub-id pub-id-type="doi">10.1097/MS9.0000000000000188</pub-id><pub-id pub-id-type="pmid">36845786</pub-id></citation></ref>
<ref id="B55">
<label>55.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Alemu</surname> <given-names>A</given-names></name> <name><surname>Bitew</surname> <given-names>ZW</given-names></name> <name><surname>Seid</surname> <given-names>G</given-names></name> <name><surname>Diriba</surname> <given-names>G</given-names></name> <name><surname>Gashu</surname> <given-names>E</given-names></name> <name><surname>Berhe</surname> <given-names>N</given-names></name> <etal/></person-group>. <article-title>Tuberculosis in individuals who recovered from COVID-19: a systematic review of case reports</article-title>. <source>PLoS ONE.</source> (<year>2022</year>) <volume>17</volume>:<fpage>e0277807</fpage>. <pub-id pub-id-type="doi">10.1371/journal.pone.0277807</pub-id><pub-id pub-id-type="pmid">36441785</pub-id></citation></ref>
<ref id="B56">
<label>56.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Pathak</surname> <given-names>L</given-names></name> <name><surname>Gayan</surname> <given-names>S</given-names></name> <name><surname>Pal</surname> <given-names>B</given-names></name> <name><surname>Talukdar</surname> <given-names>J</given-names></name> <name><surname>Bhuyan</surname> <given-names>S</given-names></name> <name><surname>Sandhya</surname> <given-names>S</given-names></name> <etal/></person-group>. <article-title>Coronavirus activates an altruistic stem cell-mediated defense mechanism that reactivates dormant tuberculosis implications in coronavirus disease 2019 pandemic</article-title>. <source>Am J Pathol.</source> (<year>2021</year>) <volume>191</volume>:<fpage>1255</fpage>&#x02013;<lpage>68</lpage>. <pub-id pub-id-type="doi">10.1016/j.ajpath.2021.03.011</pub-id><pub-id pub-id-type="pmid">33887214</pub-id></citation></ref>
<ref id="B57">
<label>57.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Khayat</surname> <given-names>M</given-names></name> <name><surname>Fan</surname> <given-names>H</given-names></name> <name><surname>Vali</surname> <given-names>Y</given-names></name></person-group>. <article-title>COVID-19 promoting the development of active tuberculosis in a patient with latent tuberculosis infection: a case report</article-title>. <source>Respir Med Case Rep.</source> (<year>2021</year>) <volume>32</volume>:<fpage>101344</fpage>. <pub-id pub-id-type="doi">10.1016/j.rmcr.2021.101344</pub-id><pub-id pub-id-type="pmid">33495728</pub-id></citation></ref>
<ref id="B58">
<label>58.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Silva</surname> <given-names>DR</given-names></name></person-group>. <article-title>Mello FCd, D&#x00027;Ambrosio L, Centis R, Dalcolmo MP, Migliori GB. Tuberculosis and COVID-19, the new cursed duet: what differs between Brazil and Europe?</article-title> <source>J Bras Pneumol.</source> (<year>2021</year>) <volume>47</volume>:<fpage>e20210044</fpage>. <pub-id pub-id-type="doi">10.36416/1806-3756/e20210044</pub-id><pub-id pub-id-type="pmid">33950095</pub-id></citation></ref>
<ref id="B59">
<label>59.</label>
<citation citation-type="journal"><person-group person-group-type="author"><collab>TB/COVID-19 Global Study Group</collab></person-group>. <article-title>Tuberculosis and COVID-19 co-infection: description of the global cohort</article-title>. <source>Eur Respir J</source>. (<year>2022</year>) <volume>59</volume>:<fpage>2102538</fpage>. <pub-id pub-id-type="doi">10.1183/13993003.02538-2021</pub-id></citation>
</ref>
<ref id="B60">
<label>60.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Wang</surname> <given-names>Y</given-names></name> <name><surname>Feng</surname> <given-names>R</given-names></name> <name><surname>Xu</surname> <given-names>J</given-names></name> <name><surname>Hou</surname> <given-names>H</given-names></name> <name><surname>Feng</surname> <given-names>H</given-names></name> <name><surname>Yang</surname> <given-names>H</given-names></name></person-group>. <article-title>An updated meta-analysis on the association between tuberculosis and COVID-19 severity and mortality</article-title>. <source>J Med Virol.</source> (<year>2021</year>) <volume>93</volume>:<fpage>5682</fpage>&#x02013;<lpage>6</lpage>. <pub-id pub-id-type="doi">10.1002/jmv.27119</pub-id><pub-id pub-id-type="pmid">34061374</pub-id></citation></ref>
<ref id="B61">
<label>61.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Chen</surname> <given-names>Y</given-names></name> <name><surname>Wang</surname> <given-names>Y</given-names></name> <name><surname>Fleming</surname> <given-names>J</given-names></name> <name><surname>Yu</surname> <given-names>Y</given-names></name> <name><surname>Gu</surname> <given-names>Y</given-names></name> <name><surname>Liu</surname> <given-names>C</given-names></name> <etal/></person-group>. <article-title>Active or latent tuberculosis increases susceptibility to COVID-19 and disease severity</article-title>. <source>medRxiv</source>. <pub-id pub-id-type="doi">10.1101/2020.03.10.20033795</pub-id></citation>
</ref>
<ref id="B62">
<label>62.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Ong</surname> <given-names>CWM</given-names></name> <name><surname>Migliori</surname> <given-names>GB</given-names></name> <name><surname>Raviglione</surname> <given-names>M</given-names></name> <name><surname>MacGregor-Skinner</surname> <given-names>G</given-names></name> <name><surname>Sotgiu</surname> <given-names>G</given-names></name> <name><surname>Alffenaar</surname> <given-names>J-W</given-names></name> <etal/></person-group>. <article-title>Epidemic and pandemic viral infections: impact on tuberculosis and the lung: A consensus by the World Association for Infectious Diseases and Immunological Disorders (WAidid), Global Tuberculosis Network (GTN), and members of the European Society of Clinical Microbiology and Infectious Diseases Study Group for Mycobacterial Infections (ESGMYC)</article-title>. <source>Eur Respir J</source>. (<year>2020</year>) <fpage>56</fpage>. <pub-id pub-id-type="doi">10.1183/13993003.01727-2020</pub-id></citation>
</ref>
<ref id="B63">
<label>63.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Bass</surname> <given-names>SN</given-names></name> <name><surname>Spagnuolo</surname> <given-names>PJ</given-names></name> <name><surname>Ellner</surname> <given-names>JJ</given-names></name></person-group>. <article-title>Augmented neutrophil adherence in active and remote tuberculosis</article-title>. <source>Am Rev Respir Dis.</source> (<year>1981</year>) <volume>124</volume>:<fpage>643</fpage>&#x02013;<lpage>5</lpage>.<pub-id pub-id-type="pmid">7030159</pub-id></citation></ref>
<ref id="B64">
<label>64.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Canaday</surname> <given-names>DH</given-names></name> <name><surname>Sridaran</surname> <given-names>S</given-names></name> <name><surname>Van Epps</surname> <given-names>P</given-names></name> <name><surname>Aung</surname> <given-names>H</given-names></name> <name><surname>Burant</surname> <given-names>CJ</given-names></name> <name><surname>Nsereko</surname> <given-names>M</given-names></name> <etal/></person-group>. <article-title>CD4&#x0002B; T cell polyfunctional profile in HIV-TB coinfection are similar between individuals with latent and active TB infection</article-title>. <source>Tuberculosis.</source> (<year>2015</year>) <volume>95</volume>:<fpage>470</fpage>&#x02013;<lpage>5</lpage>. <pub-id pub-id-type="doi">10.1016/j.tube.2014.12.008</pub-id><pub-id pub-id-type="pmid">25956974</pub-id></citation></ref>
<ref id="B65">
<label>65.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Sullivan</surname> <given-names>ZA</given-names></name> <name><surname>Wong</surname> <given-names>EB</given-names></name> <name><surname>Ndung&#x00027;u</surname> <given-names>T</given-names></name> <name><surname>Kasprowicz</surname> <given-names>VO</given-names></name> <name><surname>Bishai</surname> <given-names>WR</given-names></name></person-group>. <article-title>Latent and active tuberculosis infection increase immune activation in individuals co-infected with HIV</article-title>. <source>EBioMedicine.</source> (<year>2015</year>) <volume>2</volume>:<fpage>334</fpage>&#x02013;<lpage>40</lpage>. <pub-id pub-id-type="doi">10.1016/j.ebiom.2015.03.005</pub-id><pub-id pub-id-type="pmid">26114158</pub-id></citation></ref>
<ref id="B66">
<label>66.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Malherbe</surname> <given-names>ST</given-names></name> <name><surname>Shenai</surname> <given-names>S</given-names></name> <name><surname>Ronacher</surname> <given-names>K</given-names></name> <name><surname>Loxton</surname> <given-names>AG</given-names></name> <name><surname>Dolganov</surname> <given-names>G</given-names></name> <name><surname>Kriel</surname> <given-names>M</given-names></name> <etal/></person-group>. <article-title>Persisting positron emission tomography lesion activity and Mycobacterium tuberculosis mRNA after tuberculosis cure</article-title>. <source>Nat Med.</source> (<year>2016</year>) <volume>22</volume>:<fpage>1094</fpage>&#x02013;<lpage>100</lpage>. <pub-id pub-id-type="doi">10.1038/nm.4177</pub-id><pub-id pub-id-type="pmid">27595324</pub-id></citation></ref>
<ref id="B67">
<label>67.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Sy</surname> <given-names>KTL</given-names></name> <name><surname>Haw</surname> <given-names>NJL</given-names></name> <name><surname>Uy</surname> <given-names>J</given-names></name></person-group>. <article-title>Previous and active tuberculosis increases risk of death and prolongs recovery in patients with COVID-19</article-title>. <source>Infect Dis.</source> (<year>2020</year>) <volume>52</volume>:<fpage>902</fpage>&#x02013;<lpage>7</lpage>. <pub-id pub-id-type="doi">10.1080/23744235.2020.1806353</pub-id><pub-id pub-id-type="pmid">32808838</pub-id></citation></ref>
<ref id="B68">
<label>68.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Kleinnijenhuis</surname> <given-names>J</given-names></name> <name><surname>Quintin</surname> <given-names>J</given-names></name> <name><surname>Preijers</surname> <given-names>F</given-names></name> <name><surname>Joosten</surname> <given-names>LAB</given-names></name> <name><surname>Ifrim</surname> <given-names>DC</given-names></name> <name><surname>Saeed</surname> <given-names>S</given-names></name> <etal/></person-group>. <article-title>Bacille Calmette-Guerin induces NOD2-dependent nonspecific protection from reinfection via epigenetic reprogramming of monocytes</article-title>. <source>Proc Natl Acad Sci USA.</source> (<year>2012</year>) <volume>109</volume>:<fpage>17537</fpage>&#x02013;<lpage>42</lpage>. <pub-id pub-id-type="doi">10.1073/pnas.1202870109</pub-id><pub-id pub-id-type="pmid">22988082</pub-id></citation></ref>
<ref id="B69">
<label>69.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Kleinnijenhuis</surname> <given-names>J</given-names></name> <name><surname>Quintin</surname> <given-names>J</given-names></name> <name><surname>Preijers</surname> <given-names>F</given-names></name> <name><surname>Joosten</surname> <given-names>LA</given-names></name> <name><surname>Jacobs</surname> <given-names>C</given-names></name> <name><surname>Xavier</surname> <given-names>RJ</given-names></name> <etal/></person-group>. <article-title>BCG-induced trained immunity in NK cells: Role for non-specific protection to infection</article-title>. <source>Clin Immunol.</source> (<year>2014</year>) <volume>155</volume>:<fpage>213</fpage>&#x02013;<lpage>9</lpage>. <pub-id pub-id-type="doi">10.1016/j.clim.2014.10.005</pub-id><pub-id pub-id-type="pmid">25451159</pub-id></citation></ref>
<ref id="B70">
<label>70.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Joosten</surname> <given-names>SA</given-names></name> <name><surname>van Meijgaarden</surname> <given-names>KE</given-names></name> <name><surname>Arend</surname> <given-names>SM</given-names></name> <name><surname>Prins</surname> <given-names>C</given-names></name> <name><surname>Oftung</surname> <given-names>F</given-names></name> <name><surname>Korsvold</surname> <given-names>GE</given-names></name> <etal/></person-group>. <article-title>Mycobacterial growth inhibition is associated with trained innate immunity</article-title>. <source>J Clin Investig.</source> (<year>2018</year>) <volume>128</volume>:<fpage>1837</fpage>&#x02013;<lpage>51</lpage>. <pub-id pub-id-type="doi">10.1172/JCI97508</pub-id><pub-id pub-id-type="pmid">29461976</pub-id></citation></ref>
<ref id="B71">
<label>71.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Gupta</surname> <given-names>A</given-names></name> <name><surname>Sural</surname> <given-names>S</given-names></name> <name><surname>Gupta</surname> <given-names>A</given-names></name> <name><surname>Rousa</surname> <given-names>S</given-names></name> <name><surname>Koner</surname> <given-names>BC</given-names></name> <name><surname>Bhalotra</surname> <given-names>A</given-names></name> <etal/></person-group>. <article-title>Positive QuantiFERON test and the severity of COVID-19 disease: a prospective study</article-title>. <source>Indian J Tuberc.</source> (<year>2021</year>) <volume>68</volume>:<fpage>474</fpage>&#x02013;<lpage>80</lpage>. <pub-id pub-id-type="doi">10.1016/j.ijtb.2020.12.013</pub-id><pub-id pub-id-type="pmid">34752316</pub-id></citation></ref>
<ref id="B72">
<label>72.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Gulen</surname> <given-names>TA</given-names></name> <name><surname>Bayraktar</surname> <given-names>M</given-names></name> <name><surname>Yaksi</surname> <given-names>N</given-names></name> <name><surname>Kayabas</surname> <given-names>U</given-names></name></person-group>. <article-title>Is the course of COVID-19 associated with tuberculin skin test diameter? A retrospective study</article-title>. <source>J Med Virol.</source> (<year>2022</year>) <volume>94</volume>:<fpage>1020</fpage>&#x02013;<lpage>6</lpage>. <pub-id pub-id-type="doi">10.1002/jmv.27414</pub-id><pub-id pub-id-type="pmid">34676582</pub-id></citation></ref>
<ref id="B73">
<label>73.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Khan</surname> <given-names>N</given-names></name> <name><surname>Downey</surname> <given-names>J</given-names></name> <name><surname>Sanz</surname> <given-names>J</given-names></name> <name><surname>Kaufmann</surname> <given-names>E</given-names></name> <name><surname>Blankenhaus</surname> <given-names>B</given-names></name> <name><surname>Pacis</surname> <given-names>A</given-names></name> <etal/></person-group>. <article-title>M. tuberculosis reprograms hematopoietic stem cells to limit myelopoiesis and impair trained immunity</article-title>. <source>Cell</source>. (<year>2020</year>) 183:752&#x02013;70 e22. <pub-id pub-id-type="doi">10.1016/j.cell.2020.09.062</pub-id></citation>
</ref>
<ref id="B74">
<label>74.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Mejia</surname> <given-names>OR</given-names></name> <name><surname>Gloag ES Li</surname> <given-names>J</given-names></name> <name><surname>Ruane-Foster</surname> <given-names>M</given-names></name> <name><surname>Claeys</surname> <given-names>TA</given-names></name> <name><surname>Farkas</surname> <given-names>D</given-names></name> <etal/></person-group>. <article-title>Mice infected with <italic>Mycobacterium tuberculosis</italic> are resistant to acute disease caused by secondary infection with SARS-CoV-2</article-title>. <source>PLoS Pathog.</source> (<year>2022</year>) <volume>18</volume>:<fpage>e1010093</fpage>. <pub-id pub-id-type="doi">10.1371/journal.ppat.1010093</pub-id><pub-id pub-id-type="pmid">35325013</pub-id></citation></ref>
<ref id="B75">
<label>75.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Butkeviciute</surname> <given-names>E</given-names></name> <name><surname>Jones</surname> <given-names>CE</given-names></name> <name><surname>Smith</surname> <given-names>SG</given-names></name></person-group>. <article-title>Heterologous effects of infant BCG vaccination: potential mechanisms of immunity</article-title>. <source>Future Microbiol.</source> (<year>2018</year>) <volume>13</volume>:<fpage>1193</fpage>&#x02013;<lpage>208</lpage>. <pub-id pub-id-type="doi">10.2217/fmb-2018-0026</pub-id><pub-id pub-id-type="pmid">30117744</pub-id></citation></ref>
<ref id="B76">
<label>76.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>de Bree</surname> <given-names>LCJ</given-names></name> <name><surname>Koeken</surname> <given-names>VACM</given-names></name> <name><surname>Joosten</surname> <given-names>LAB</given-names></name> <name><surname>Aaby</surname> <given-names>P</given-names></name> <name><surname>Benn</surname> <given-names>CS</given-names></name> <name><surname>van Crevel</surname> <given-names>R</given-names></name> <etal/></person-group>. <article-title>Non-specific effects of vaccines: Current evidence and potential implications</article-title>. <source>Semin Immunol.</source> (<year>2018</year>) <volume>39</volume>:<fpage>35</fpage>&#x02013;<lpage>43</lpage>. <pub-id pub-id-type="doi">10.1016/j.smim.2018.06.002</pub-id><pub-id pub-id-type="pmid">30007489</pub-id></citation></ref>
<ref id="B77">
<label>77.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Koeken</surname> <given-names>V</given-names></name> <name><surname>Verrall</surname> <given-names>AJ</given-names></name> <name><surname>Netea</surname> <given-names>MG</given-names></name> <name><surname>Hill</surname> <given-names>PC</given-names></name> <name><surname>van Crevel</surname> <given-names>R</given-names></name></person-group>. <article-title>Trained innate immunity and resistance to Mycobacterium tuberculosis infection</article-title>. <source>Clin Microbiol Infect.</source> (<year>2019</year>) <volume>25</volume>:<fpage>1468</fpage>&#x02013;<lpage>72</lpage>. <pub-id pub-id-type="doi">10.1016/j.cmi.2019.02.015</pub-id><pub-id pub-id-type="pmid">30807849</pub-id></citation></ref>
<ref id="B78">
<label>78.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Moorlag</surname> <given-names>S</given-names></name> <name><surname>R&#x000F6;ring</surname> <given-names>RJ</given-names></name> <name><surname>Joosten</surname> <given-names>LAB</given-names></name> <name><surname>Netea</surname> <given-names>MG</given-names></name></person-group>. <article-title>The role of the interleukin-1 family in trained immunity</article-title>. <source>Immunol Rev.</source> (<year>2018</year>) <volume>281</volume>:<fpage>28</fpage>&#x02013;<lpage>39</lpage>. <pub-id pub-id-type="doi">10.1111/imr.12617</pub-id><pub-id pub-id-type="pmid">29248003</pub-id></citation></ref>
<ref id="B79">
<label>79.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Arts</surname> <given-names>RJW</given-names></name> <name><surname>Moorlag</surname> <given-names>SJCFM</given-names></name> <name><surname>Novakovic</surname> <given-names>B</given-names></name> <name><surname>Li</surname> <given-names>Y</given-names></name> <name><surname>Wang</surname> <given-names>SY</given-names></name> <name><surname>Oosting</surname> <given-names>M</given-names></name> <etal/></person-group>. <article-title>BCG vaccination protects against experimental viral infection in humans through the induction of cytokines associated with trained immunity</article-title>. <source>Cell Host Microbe</source>. (<year>2018</year>) <volume>23</volume>:<fpage>89</fpage>. <pub-id pub-id-type="doi">10.1016/j.chom.2017.12.010</pub-id><pub-id pub-id-type="pmid">29324233</pub-id></citation></ref>
<ref id="B80">
<label>80.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Kaufmann</surname> <given-names>E</given-names></name> <name><surname>Sanz</surname> <given-names>J</given-names></name> <name><surname>Dunn</surname> <given-names>JL</given-names></name> <name><surname>Khan</surname> <given-names>N</given-names></name> <name><surname>Mendon&#x000E7;a</surname> <given-names>LE</given-names></name> <name><surname>Pacis</surname> <given-names>A</given-names></name> <etal/></person-group>. <article-title>BCG educates hematopoietic stem cells to generate protective innate immunity against tuberculosis</article-title>. <source>Cell</source>. (<year>2018</year>) 172:176&#x02013;90 e19. <pub-id pub-id-type="doi">10.1016/j.cell.2017.12.031</pub-id><pub-id pub-id-type="pmid">29328912</pub-id></citation></ref>
<ref id="B81">
<label>81.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Netea</surname> <given-names>MG</given-names></name> <name><surname>Giamarellos-Bourboulis</surname> <given-names>EJ</given-names></name> <name><surname>Dom&#x000ED;nguez-Andr&#x000E9;s</surname> <given-names>J</given-names></name> <name><surname>Curtis</surname> <given-names>N</given-names></name> <name><surname>van Crevel</surname> <given-names>R</given-names></name> <name><surname>van de Veerdonk</surname> <given-names>FL</given-names></name> <etal/></person-group>. <article-title>Trained immunity: a tool for reducing susceptibility to and the severity of SARS-CoV-2</article-title>. <source>Infect Cell.</source> (<year>2020</year>) <volume>181</volume>:<fpage>969</fpage>&#x02013;<lpage>77</lpage>. <pub-id pub-id-type="doi">10.1016/j.cell.2020.04.042</pub-id><pub-id pub-id-type="pmid">32437659</pub-id></citation></ref>
<ref id="B82">
<label>82.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>O&#x00027;Neill</surname> <given-names>LAJ</given-names></name> <name><surname>Netea</surname> <given-names>MG</given-names></name></person-group>. <article-title>BCG-induced trained immunity: can it offer protection against COVID-19?</article-title> <source>Nat Rev Immunol.</source> (<year>2020</year>) <volume>20</volume>:<fpage>335</fpage>&#x02013;<lpage>7</lpage>. <pub-id pub-id-type="doi">10.1038/s41577-020-0337-y</pub-id><pub-id pub-id-type="pmid">32393823</pub-id></citation></ref>
<ref id="B83">
<label>83.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Torun</surname> <given-names>S</given-names></name> <name><surname>Ozkaya</surname> <given-names>S</given-names></name> <name><surname>Sen</surname> <given-names>N</given-names></name> <name><surname>Kanat</surname> <given-names>F</given-names></name> <name><surname>Karaman</surname> <given-names>I</given-names></name> <name><surname>Yosunkaya</surname> <given-names>S</given-names></name> <etal/></person-group>. <article-title>The relationship between COVID-19 severity and Bacillus Calmette-Gu&#x000E9;rin (BCG)/<italic>Mycobacterium tuberculosis</italic> exposure history in healthcare workers: a multi-center study</article-title>. <source>Pathog Glob Health.</source> (<year>2021</year>) <volume>115</volume>:<fpage>405</fpage>&#x02013;<lpage>11</lpage>. <pub-id pub-id-type="doi">10.1080/20477724.2021.1927605</pub-id><pub-id pub-id-type="pmid">34014806</pub-id></citation></ref>
<ref id="B84">
<label>84.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Dos Anjos</surname> <given-names>LRB</given-names></name> <name><surname>da Costa</surname> <given-names>AC</given-names></name> <name><surname>Cardoso</surname> <given-names>AdO</given-names></name> <name><surname>Guimar&#x000E3;es</surname> <given-names>RA</given-names></name> <name><surname>Rodrigues</surname> <given-names>RL</given-names></name> <name><surname>Ribeiro</surname> <given-names>KM</given-names></name> <etal/></person-group>. <article-title>Efficacy and safety of BCG revaccination with <italic>M. bovis</italic> BCG Moscow to prevent COVID-19 infection in health care workers: a randomized phase II clinical trial</article-title>. <source>Front Immunol.</source> (<year>2022</year>) <volume>13</volume>:<fpage>841868</fpage>. <pub-id pub-id-type="doi">10.3389/fimmu.2022.841868</pub-id><pub-id pub-id-type="pmid">35392074</pub-id></citation></ref>
<ref id="B85">
<label>85.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Rivas</surname> <given-names>MN</given-names></name> <name><surname>Ebinger</surname> <given-names>JE</given-names></name> <name><surname>Wu</surname> <given-names>M</given-names></name> <name><surname>Sun</surname> <given-names>N</given-names></name> <name><surname>Braun</surname> <given-names>J</given-names></name> <name><surname>Sobhani</surname> <given-names>K</given-names></name> <etal/></person-group>. <article-title>BCG vaccination history associates with decreased SARS-CoV-2 seroprevalence across a diverse cohort of health care workers</article-title>. <source>J Clin Invest</source>. (<year>2021</year>) <volume>131</volume>:<fpage>e145157</fpage>. <pub-id pub-id-type="doi">10.1172/JCI145157</pub-id><pub-id pub-id-type="pmid">33211672</pub-id></citation></ref>
<ref id="B86">
<label>86.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Blossey</surname> <given-names>AM</given-names></name> <name><surname>Br&#x000FC;ckner</surname> <given-names>S</given-names></name> <name><surname>May</surname> <given-names>M</given-names></name> <name><surname>Parzmair</surname> <given-names>GP</given-names></name> <name><surname>Sharma</surname> <given-names>H</given-names></name> <name><surname>Shaligram</surname> <given-names>U</given-names></name> <etal/></person-group>. <article-title>VPM1002 as prophylaxis against severe respiratory tract infections including coronavirus disease 2019 in the elderly: a phase 3 randomized, double-blind, placebo-controlled, multicenter clinical study</article-title>. <source>Clin Infect Dis.</source> (<year>2023</year>) <volume>76</volume>:<fpage>1304</fpage>&#x02013;<lpage>10</lpage>. <pub-id pub-id-type="doi">10.1093/cid/ciac881</pub-id><pub-id pub-id-type="pmid">36358012</pub-id></citation></ref>
<ref id="B87">
<label>87.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Wen</surname> <given-names>J</given-names></name> <name><surname>Liu</surname> <given-names>Q</given-names></name> <name><surname>Tang</surname> <given-names>D</given-names></name> <name><surname>He</surname> <given-names>J-Q</given-names></name></person-group>. <article-title>Efficacy of BCG vaccination against COVID-19: systematic review and meta-analysis of randomized controlled trials</article-title>. <source>J Clin Med</source>. (<year>2023</year>) <volume>12</volume>:<fpage>1154</fpage>. <pub-id pub-id-type="doi">10.3390/jcm12031154</pub-id><pub-id pub-id-type="pmid">36769802</pub-id></citation></ref>
<ref id="B88">
<label>88.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Claus</surname> <given-names>J</given-names></name> <name><surname>Kollan</surname> <given-names>C</given-names></name> <name><surname>Gunsenheimer-Bartmeyer</surname> <given-names>B</given-names></name> <name><surname>Jensen</surname> <given-names>BO</given-names></name> <name><surname>Stephan</surname> <given-names>C</given-names></name> <name><surname>Degen</surname> <given-names>O</given-names></name> <etal/></person-group>. <article-title>BCG vaccination of health care workers does not reduce SARS-CoV-2 infections nor infection severity or duration: a randomized placebo-controlled</article-title>. <source>Trial mBio.</source> (<year>2023</year>) <volume>14</volume>:<fpage>e0035623</fpage>. <pub-id pub-id-type="doi">10.1101/2022.12.12.22283282</pub-id><pub-id pub-id-type="pmid">36976004</pub-id></citation></ref>
<ref id="B89">
<label>89.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Pittet</surname> <given-names>LF</given-names></name> <name><surname>Messina</surname> <given-names>NL</given-names></name> <name><surname>Orsini</surname> <given-names>F</given-names></name> <name><surname>Moore</surname> <given-names>CL</given-names></name> <name><surname>Abruzzo</surname> <given-names>V</given-names></name> <name><surname>Barry</surname> <given-names>S</given-names></name> <etal/></person-group>. <article-title>Randomized trial of BCG vaccine to protect against Covid-19 in health care workers</article-title>. <source>N Engl J Med.</source> (<year>2023</year>) <volume>388</volume>:<fpage>1582</fpage>&#x02013;<lpage>96</lpage>. <pub-id pub-id-type="doi">10.1056/NEJMoa2212616</pub-id><pub-id pub-id-type="pmid">37099341</pub-id></citation></ref>
<ref id="B90">
<label>90.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Moorlag</surname> <given-names>S</given-names></name> <name><surname>Taks</surname> <given-names>E</given-names></name> <name><surname>Ten Doesschate</surname> <given-names>T</given-names></name> <name><surname>van der Vaart</surname> <given-names>TW</given-names></name> <name><surname>Janssen</surname> <given-names>AB</given-names></name> <name><surname>M&#x000FC;ller</surname> <given-names>L</given-names></name> <etal/></person-group>. <article-title>Efficacy of BCG vaccination against respiratory tract infections in older adults during the coronavirus disease 2019 pandemic</article-title>. <source>Clin Infect Dis</source>. (<year>2022</year>) <volume>75</volume>:<fpage>e938</fpage>&#x02013;<lpage>46</lpage>. <pub-id pub-id-type="doi">10.1093/cid/ciac182</pub-id><pub-id pub-id-type="pmid">35247264</pub-id></citation></ref>
<ref id="B91">
<label>91.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Upton</surname> <given-names>CM</given-names></name> <name><surname>van Wijk</surname> <given-names>RC</given-names></name> <name><surname>Mockeliunas</surname> <given-names>L</given-names></name> <name><surname>Simonsson</surname> <given-names>USH</given-names></name> <name><surname>McHarry</surname> <given-names>K</given-names></name> <name><surname>van den Hoogen</surname> <given-names>G</given-names></name> <etal/></person-group>. <article-title>Safety and efficacy of BCG re-vaccination in relation to COVID-19 morbidity in healthcare workers: a double-blind, randomised, controlled, phase 3 trial</article-title>. <source>EClinicalMedicine.</source> (<year>2022</year>) <volume>48</volume>:<fpage>101414</fpage>. <pub-id pub-id-type="doi">10.1016/j.eclinm.2022.101414</pub-id><pub-id pub-id-type="pmid">35582122</pub-id></citation></ref>
<ref id="B92">
<label>92.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Ten Doesschate</surname> <given-names>T</given-names></name> <name><surname>van der Vaart</surname> <given-names>TW</given-names></name> <name><surname>Debisarun</surname> <given-names>PA</given-names></name> <name><surname>Taks</surname> <given-names>E</given-names></name> <name><surname>Moorlag</surname> <given-names>SJCFM</given-names></name> <name><surname>Paternotte</surname> <given-names>N</given-names></name> <etal/></person-group>. <article-title>Bacillus Calmette-Guerin vaccine to reduce healthcare worker absenteeism in COVID-19 pandemic, a randomized controlled trial</article-title>. <source>Clin Microbiol Infect.</source> (<year>2022</year>) <volume>28</volume>:<fpage>1278</fpage>&#x02013;<lpage>85</lpage>. <pub-id pub-id-type="doi">10.1016/j.cmi.2022.04.009</pub-id><pub-id pub-id-type="pmid">35489606</pub-id></citation></ref>
<ref id="B93">
<label>93.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Czajka</surname> <given-names>H</given-names></name> <name><surname>Zapolnik</surname> <given-names>P</given-names></name> <name><surname>Krzych</surname> <given-names>&#x00141;</given-names></name> <name><surname>Kmiecik</surname> <given-names>W</given-names></name> <name><surname>Stopyra</surname> <given-names>L</given-names></name> <name><surname>Nowakowska</surname> <given-names>A</given-names></name> <etal/></person-group>. <article-title>A multi-center, randomised, double-blind, placebo-controlled phase III clinical trial evaluating the impact of BCG re-vaccination on the incidence and severity of SARS-CoV-2 infections among symptomatic healthcare professionals during the COVID-19 pandemic in Poland-First Results</article-title>. <source>Vaccines</source>. (<year>2022</year>) <fpage>10</fpage>. <pub-id pub-id-type="doi">10.3390/vaccines10020314</pub-id></citation>
</ref>
<ref id="B94">
<label>94.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Gong</surname> <given-names>W</given-names></name> <name><surname>Liu</surname> <given-names>Y</given-names></name> <name><surname>Xue</surname> <given-names>Y</given-names></name> <name><surname>Zhuang</surname> <given-names>L</given-names></name></person-group>. <article-title>Two issues should be noted when designing a clinical trial to evaluate BCG effects on COVID-19</article-title>. <source>Front Immunol.</source> (<year>2023</year>) <volume>14</volume>:<fpage>1207212</fpage>. <pub-id pub-id-type="doi">10.3389/fimmu.2023.1207212</pub-id><pub-id pub-id-type="pmid">37287961</pub-id></citation></ref>
<ref id="B95">
<label>95.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Aaby</surname> <given-names>P</given-names></name> <name><surname>Netea</surname> <given-names>MG</given-names></name> <name><surname>Benn</surname> <given-names>CS</given-names></name></person-group>. <article-title>Beneficial non-specific effects of live vaccines against COVID-19 and other unrelated infections</article-title>. <source>Lancet Infect Dis.</source> (<year>2023</year>) <volume>23</volume>:<fpage>e34</fpage>&#x02013;<lpage>42</lpage>. <pub-id pub-id-type="doi">10.1016/S1473-3099(22)00498-4</pub-id><pub-id pub-id-type="pmid">36037824</pub-id></citation></ref>
<ref id="B96">
<label>96.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Sinha</surname> <given-names>S</given-names></name> <etal/></person-group>. <article-title>Efficacy of Bacillus Calmette-Guerin (BCG) vaccination in reducing the incidence and severity of COVID-19 in high-risk population (BRIC): a phase III, multi-centre, quadruple-blind randomised control trial</article-title>. <source>Infect Dis Ther.</source> (<year>2022</year>) <volume>11</volume>:<fpage>2205</fpage>&#x02013;<lpage>17</lpage>. <pub-id pub-id-type="doi">10.1007/s40121-022-00703-y</pub-id><pub-id pub-id-type="pmid">36242739</pub-id></citation></ref>
<ref id="B97">
<label>97.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Faustman</surname> <given-names>DL</given-names></name> <name><surname>Lee</surname> <given-names>A</given-names></name> <name><surname>Hostetter</surname> <given-names>ER</given-names></name> <name><surname>Aristarkhova</surname> <given-names>A</given-names></name> <name><surname>Ng</surname> <given-names>NC</given-names></name> <name><surname>Shpilsky</surname> <given-names>GF</given-names></name> <etal/></person-group>. <article-title>Multiple BCG vaccinations for the prevention of COVID-19 and other infectious diseases in type 1 diabetes</article-title>. <source>Cell Rep Med.</source> (<year>2022</year>) <volume>3</volume>:<fpage>100728</fpage>. <pub-id pub-id-type="doi">10.1016/j.xcrm.2022.100728</pub-id><pub-id pub-id-type="pmid">36027906</pub-id></citation></ref>
<ref id="B98">
<label>98.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Santos</surname> <given-names>AP</given-names></name> <name><surname>Werneck</surname> <given-names>GL</given-names></name> <name><surname>Dalvi</surname> <given-names>APR</given-names></name> <name><surname>Dos Santos</surname> <given-names>CC</given-names></name> <name><surname>Tierno</surname> <given-names>PFGMM</given-names></name> <name><surname>Condelo</surname> <given-names>HS</given-names></name> <etal/></person-group>. <article-title>The effect of BCG vaccination on infection and antibody levels against SARS-CoV-2-The results of ProBCG: a multicenter randomized clinical trial in Brazil</article-title>. <source>Int J Infect Dis.</source> (<year>2023</year>) <volume>130</volume>:<fpage>8</fpage>&#x02013;<lpage>16</lpage>. <pub-id pub-id-type="doi">10.1016/j.ijid.2023.02.014</pub-id><pub-id pub-id-type="pmid">36841502</pub-id></citation></ref>
<ref id="B99">
<label>99.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Darrah</surname> <given-names>PA</given-names></name> <name><surname>Zeppa</surname> <given-names>JJ</given-names></name> <name><surname>Maiello</surname> <given-names>P</given-names></name> <name><surname>Hackney</surname> <given-names>JA</given-names></name> <name><surname>Wadsworth MH</surname> <given-names>II</given-names></name> <name><surname>Hughes</surname> <given-names>TK</given-names></name> <etal/></person-group>. <article-title>Prevention of tuberculosis in macaques after intravenous BCG immunization</article-title>. <source>Nature.</source> (<year>2020</year>) <volume>577</volume>:<fpage>95</fpage>&#x02013;<lpage>102</lpage>. <pub-id pub-id-type="doi">10.1038/s41586-019-1817-8</pub-id><pub-id pub-id-type="pmid">31894150</pub-id></citation></ref>
<ref id="B100">
<label>100.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Larson</surname> <given-names>EC</given-names></name> <name><surname>Ellis-Connell</surname> <given-names>AL</given-names></name> <name><surname>Rodgers</surname> <given-names>MA</given-names></name> <name><surname>Gubernat</surname> <given-names>AK</given-names></name> <name><surname>Gleim</surname> <given-names>JL</given-names></name> <name><surname>Moriarty</surname> <given-names>RV</given-names></name> <etal/></person-group>. <article-title>Intravenous Bacille Calmette-Gu&#x000E9;rin vaccination protects simian immunodeficiency virus-infected macaques from tuberculosis</article-title>. <source>Nat Microbiol</source>. (<year>2023</year>) <volume>8</volume>:<fpage>2080</fpage>&#x02013;<lpage>92</lpage>. <pub-id pub-id-type="doi">10.1038/s41564-023-01503-x</pub-id><pub-id pub-id-type="pmid">37814073</pub-id></citation></ref>
<ref id="B101">
<label>101.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Tran</surname> <given-names>KA</given-names></name> <name><surname>Pernet</surname> <given-names>E</given-names></name> <name><surname>Sadeghi</surname> <given-names>M</given-names></name> <name><surname>Downey</surname> <given-names>J</given-names></name> <name><surname>Chronopoulos</surname> <given-names>J</given-names></name> <name><surname>Lapshina</surname> <given-names>E</given-names></name> <etal/></person-group>. <article-title>BCG immunization induces CX3CR1(hi) effector memory T cells to provide cross-protection via IFN-gamma-mediated trained immunity</article-title>. <source>Nat Immunol.</source> (<year>2024</year>) <volume>25</volume>:<fpage>418</fpage>&#x02013;<lpage>31</lpage>. <pub-id pub-id-type="doi">10.1038/s41590-023-01739-z</pub-id><pub-id pub-id-type="pmid">38225437</pub-id></citation></ref>
<ref id="B102">
<label>102.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Singh</surname> <given-names>AK</given-names></name> <name><surname>Wang</surname> <given-names>R</given-names></name> <name><surname>Lombardo</surname> <given-names>KA</given-names></name> <name><surname>Praharaj</surname> <given-names>M</given-names></name> <name><surname>Bullen</surname> <given-names>CK</given-names></name> <name><surname>Um</surname> <given-names>P</given-names></name> <etal/></person-group>. <article-title>Intravenous BCG vaccination reduces SARS-CoV-2 severity and promotes extensive reprogramming of lung immune cells</article-title>. <source>iScience.</source> (<year>2023</year>) <volume>26</volume>:<fpage>107733</fpage>. <pub-id pub-id-type="doi">10.1016/j.isci.2023.107733</pub-id><pub-id pub-id-type="pmid">37674985</pub-id></citation></ref>
<ref id="B103">
<label>103.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Hilligan</surname> <given-names>KL</given-names></name> <name><surname>Namasivayam</surname> <given-names>S</given-names></name> <name><surname>Clancy</surname> <given-names>CS</given-names></name> <name><surname>O&#x00027;Mard</surname> <given-names>D</given-names></name> <name><surname>Oland</surname> <given-names>SD</given-names></name> <name><surname>Robertson</surname> <given-names>SJ</given-names></name> <etal/></person-group>. <article-title>Intravenous administration of BCG protects mice against lethal SARS-CoV-2 challenge</article-title>. <source>J Exp Med</source>. (<year>2022</year>) <volume>219</volume>:<fpage>1862</fpage>. <pub-id pub-id-type="doi">10.1084/jem.20211862</pub-id><pub-id pub-id-type="pmid">34889942</pub-id></citation></ref>
<ref id="B104">
<label>104.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Lee</surname> <given-names>A</given-names></name> <name><surname>Floyd</surname> <given-names>K</given-names></name> <name><surname>Wu</surname> <given-names>S</given-names></name> <name><surname>Fang</surname> <given-names>Z</given-names></name> <name><surname>Tan</surname> <given-names>TK</given-names></name> <name><surname>Froggatt</surname> <given-names>HM</given-names></name> <etal/></person-group>. <article-title>BCG vaccination stimulates integrated organ immunity by feedback of the adaptive immune response to imprint prolonged innate antiviral resistance</article-title>. <source>Nat Immunol.</source> (<year>2023</year>) <volume>25</volume>:<fpage>41</fpage>&#x02013;<lpage>53</lpage>. <pub-id pub-id-type="doi">10.1038/s41590-023-01700-0</pub-id><pub-id pub-id-type="pmid">38036767</pub-id></citation></ref>
<ref id="B105">
<label>105.</label>
<citation citation-type="journal"><person-group person-group-type="author"><name><surname>Smith</surname> <given-names>AA</given-names></name> <name><surname>Su</surname> <given-names>H</given-names></name> <name><surname>Wallach</surname> <given-names>J</given-names></name> <name><surname>Liu</surname> <given-names>Y</given-names></name> <name><surname>Maiello</surname> <given-names>P</given-names></name> <name><surname>Borish</surname> <given-names>HJ</given-names></name> <etal/></person-group>. <article-title>A &#x0201C;suicide&#x0201D; BCG strain provides enhanced immunogenicity and robust protection against <italic>Mycobacterium tuberculosis</italic> in macaques</article-title>. <source>bioRxiv.</source> (<year>2023</year>). <pub-id pub-id-type="doi">10.1101/2023.11.22.568105</pub-id></citation>
</ref>
</ref-list>
</back>
</article>