AUTHOR=Tiwari Harish Kumar , Vanak Abi Tamim , O'Dea Mark , Gogoi-Tiwari Jully , Robertson Ian Duncan TITLE=A Comparative Study of Enumeration Techniques for Free-Roaming Dogs in Rural Baramati, District Pune, India JOURNAL=Frontiers in Veterinary Science VOLUME=Volume 5 - 2018 YEAR=2018 URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2018.00104 DOI=10.3389/fvets.2018.00104 ISSN=2297-1769 ABSTRACT=The presence of unvaccinated free-roaming dogs (FRD) amidst human settlements is a major contributor to the high incidence of rabies in countries where the disease is endemic, such as India. Estimating FRD population size is crucial to the planning and evaluation of interventions, such as mass immunisation, against rabies. Enumeration techniques for FRD are resource intensive and can vary from simple direct counts to statistically complex capture-recapture techniques primarily developed for ecological studies. In this study we compared eight enumeration techniques (direct count, Lincoln–Petersen’s index, Chapman’s correction estimate, Beck’s method, Schumacher-Eschmeyer method, Regression method, Huggin’s closed capture models and Application SuperDuplicates on-line tool) using data collected from Shirsuphal village of Baramati town in Western India, to recommend a technique which yields a reasonably accurate count to use for effective vaccination coverage against rabies with minimal resource inputs. A total of 263 unique dogs were sighted at least once over 6 observation occasions with no new dogs sighted on the 7th occasion. The methods that do not account for individual heterogeneity yielded population estimates in the range of 248-270, which potentially underestimate the real FRD population size. The highest estimates were obtained with the Huggin’s Mh-Jackknife (437±33), Huggin’s Mth-Chao (391±26), Huggin’s Mh-Chao (385±30), models and Application “SuperDuplicates” tool (392±20). When the sampling effort was reduced to only two surveys, the Application SuperDuplicates online tool gave the closest estimate of 349±36, which is 74% of the estimated highest population of free-roaming dogs in Shirsuphal village. This method is considered to be the most reliable method for estimating the FRD population with minimal inputs (two surveys conducted on consecutive days).