AUTHOR=Xia Zemiao , Geng Haopeng , Cai Yuan , Wang Yaonan , Sun Daquan , Zhang Jian , Pan Zhiming , Jiao Xin'an , Geng Shizhong 

TITLE=A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken

JOURNAL=Frontiers in Veterinary Science

VOLUME=Volume 7 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2020.00324

DOI=10.3389/fvets.2020.00324

ISSN=2297-1769

ABSTRACT=Salmonella enteritidis and Salmonella pullorum belonging to Group O9 Salmonella are major causative agents of infectious diseases in chicken. O9 antigen as a part of lipopolysaccharide (LPS) that is a predominant detected target for Salmonella infection. To identify their infection, an anti-O9 monoclonal antibody (McAb)-based direct competitive enzyme-linked assay (O9 Dc-ELISA) was developed after constraints were optimized, the establishment and application of O9 Dc-ELISA, compared to three commercial kits and plate agglutination test (PAT), showed that this O9 Dc-ELISA had better performances than PAT method and commercial kits in sensitivity in addition to strong specificity in the detection of clinical samples.