AUTHOR=Mekonnen Sefinew Alemu , Alelgn Zegeye , Saudik Seid , Molla Wassie , Fentie Tsegaw , Jemberu Wudu Temesgen TITLE=Reduced Milk Production, Economic Losses, and Risk Factors Associated to Subclinical Hypocalcemia in Holstein Friesian × Zebu Crossbreed Cows in North-West Ethiopia JOURNAL=Frontiers in Veterinary Science VOLUME=Volume 9 - 2022 YEAR=2022 URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2022.771889 DOI=10.3389/fvets.2022.771889 ISSN=2297-1769 ABSTRACT=Hypocalcemia is a metabolic disorder that occurs when calcium leaves the blood to support milk production or for growth of the fetus faster than calcium can be put back into the blood. Subclinical hypocalcaemia (SCH) refers to cows that are clinically normal but with concentrations of calcium lower than measured in normal cows. A study was conducted to estimate economic losses and to identify risk factors associated with SCH in Holstein Friesian × Zebu Crossbreed Cows in North-West Ethiopia. A total of 221 lactating dairy cows obtained from 81 dairy farms were selected and serum samples were collected and analyzed to estimate the level of calcium in the serum using automated electrolyte analyzer (Roch, UVL Japan, 2014). Forty seven cows obtained from 12 farms were grouped into two based on SCH status and milk yield was measured for eight consecutive days to estimate milk production loss associated with SCH. Prevalence was estimated and univariable and multivariable logistic regression analyses were used to identify determinants of SCH. The prevalence of SCH was 20.3 % (51/221, 90% CI=18.6-28.1%). Grazing on pasture, membership to dairy cooperative, feeding above the cows requirement (based on farmers’ opinion), previous experience of metritis reduced the odds of SCH (p<0.05). The average economic losses associated with reduced milk production due to SCH were 11.25 Ethiopian Birr (ETB) (1ETB = 0.023US Dollar) and 3,026.25ETB per cow per per lactation, respectively. The study has shown that SCH was a problem in crossbreed dairy cows in north-western Ethiopia and identified few risk factors that could be targeted to mitigate the problem. The economic cost of SCH was significant and needs attention in the dairy extension and dairy health training programs.