AUTHOR=Sun Qianjin , Wei Xiaona , Chen Wei , Zhong Qian , Yan Zhuanqiang , Zhou Qingfeng , Cao Yongchang , Chen Feng 

TITLE=Characterization and Evaluation of a Novel Conserved Membrane Antigen P35 of Mycoplasma synoviae

JOURNAL=Frontiers in Veterinary Science

VOLUME=Volume 9 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2022.836110

DOI=10.3389/fvets.2022.836110

ISSN=2297-1769

ABSTRACT=Mycoplasma synoviae (MS) is a major avian pathogen, which cause respiratory damage and infectious synovitis and arthritis in chickens, and cause serious economic losses to the global poultry industry. Despite its significance, the knowledge of pathogenicity and pathogenic mechanism of MS are lacked, especially its antigens. Bioinformation analysis showed that the known MS proteins are only the tip of the iceberg among many MS membrane proteins. In this study, we identified and expressed a novel MS membrane protein P35. Sequence similarity showed that P35 was conservative and commonly existed among MS strains. Membrane protein extraction and immunofluorescence assay confirmed that P35 was distributed on the surface of MS. The production of specific antibodies after immunized with recombination protein rP35 suggested the immunogenicity of it. Antigenicity of P35 was evaluated from two aspects by using polyantiserum against MS and rP35 respectively. Furthermore, to identify the immune peptides of P35, all successfully expressed truncated segments could react with positive polyantiserum of MS suggesting that P35 had more than one immune peptide. In conclusion, our study successfully identified P35 was a conservative antigen of MS, which may act as potential candidate for the future development of vaccine against MS.