AUTHOR=DeMello Victoria , Chen Grace , Wakshlag Joseph , Mason David 

TITLE=Evaluation of Platelet and Leukocyte Counts in Canine Platelet-Rich Plasma Obtained After Successive Blood Collections From the Same Patient and the Effects of Freezing on the Concentration of Growth Factors Present in It

JOURNAL=Frontiers in Veterinary Science

VOLUME=Volume 9 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2022.838481

DOI=10.3389/fvets.2022.838481

ISSN=2297-1769

ABSTRACT=Abstract:
Objective: The purpose of this study was two-fold: to evaluate whether the timing of
collection influences the platelet concentration and leukocyte counts of PRP samples, and to
evaluate consistency in growth factor concentrations in canine PRP over time after freezing and
storage without a preservation agent for 6 months of time. 
MATERIALS & METHODS: Whole blood collection was performed four times over the course
of four weeks. All PRP samples were evaluated with a CBC analysis and then stored. The PRP
samples were frozen and stored without a preservation agent using a -20º C freezer for the
duration of the 4-week study.
RESULTS: The platelet and leukocyte concentrations were not statistically significant between
the timing of blood draws over the course of 4 weeks. All three growth factors were present in
measurable quantities.
Clinical Relevance: PDGF, TGF, and VEGF were all present in measurable
quantities. Furthermore, PDGF and TGF were correlated with platelet concentration of the final
PRP. VEGF concentrations were able to be quantified. We correctly hypothesized growth factor
concentrations would be present and measurable in canine PRP frozen and stored without a
preservation agent for 6 months.