AUTHOR=Onarheim Tuva , Janczak Andrew M. , Nordgreen Janicke TITLE=The Effects of Social vs. Individual Housing of Zebrafish on Whole-Body Cortisol and Behavior in Two Tests of Anxiety JOURNAL=Frontiers in Veterinary Science VOLUME=Volume 9 - 2022 YEAR=2022 URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2022.859848 DOI=10.3389/fvets.2022.859848 ISSN=2297-1769 ABSTRACT=Two of the most commonly used models of anxiety in zebrafish research, the novel tank-diving test (NTDT) and the black-white preference test (BWPT), are modifications of assays used in rodent research (open field test and light/dark test). There has been a thorough validation of these tests in rodents, but a similar level of knowledge is still missing in zebrafish. Adult zebrafish naturally live in shoals with conspecifics, and group housing is therefore assumed to be the optimal housing condition for zebrafish, as it allows for shoaling behaviour. This study investigated how housing in social isolation affected whole-body cortisol and the behavioural responses in the NTDT and BWPT. We also examined the correlation between the behavioural responses in the two behavioural tests. We found that zebrafish housed in groups had significantly higher whole-body cortisol than individually housed zebrafish (F1,85=25.51, P<0.0001). Regardless of treatment, all groups had a general preference for the lower compartment in the NTDT and the black compartment in the BWPT. Individually housed zebrafish had a higher total number of entries to the white compartment in BWPT compared to group housed zebrafish when their first test was BWPT (F1,48=5.79, P=0.0201), but not when BWPT was their second behavioural test. Fish that had higher whole-body cortisol had a tendency towards fewer entries into the white compartment the first 3 minutes of the BWPT (F1,48=3.90, P=0.0540). There was no effect of housing on the behaviours registered in the NTDT. There was a positive correlation (correlation coefficient 0.40; p=0.003) between transitions from black to white compartment in BWPT and transitions from lower to upper compartment in NTDT, but we did not find any association between duration in white compartment in BWPT and upper compartment in NTDT. In light of this, we suggest that further model validation is needed.