AUTHOR=Stiensmeier Vera , Schmicke Marion 

TITLE=Brief Research Report: Effect of Triiodothyronine on Hepatic Growth Hormone Receptor Expression in Primary Bovine Hepatocytes

JOURNAL=Frontiers in Veterinary Science

VOLUME=Volume 9 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2022.882102

DOI=10.3389/fvets.2022.882102

ISSN=2297-1769

ABSTRACT=In previous studies triiodthyronine (T3) was found to be lower in cows with ketosis and an effect of T3 on Growth Hormone Receptor (GHR) expression is described e.g. in a human hepatoma cell line. Therefore, the aim of the present study was to test whether T3 affects GHR mRNA expression in a well-established bovine hepatocyte model. Hepatocytes were kept in a sandwich culture and stimulated for six days with constant (10 µg/ml) or decreasing (from 10 – 5 µg/ml) T3 concentrations, and GHR as well as IGF-1 mRNA expression was measured using RT-PCR. We could confirm in vitro that T3 has a stimulatory effect on GHR1A mRNA expression.