AUTHOR=Wang Yushun , Nie Mincai , Deng Huidan , Lai Siyuan , Zhou Yuancheng , Sun Xiangan , Zhu Ling , Xu Zhiwen 

TITLE=Establishment of a reverse transcription recombinase-aided amplification detection method for porcine group a rotavirus

JOURNAL=Frontiers in Veterinary Science

VOLUME=Volume 9 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2022.954657

DOI=10.3389/fvets.2022.954657

ISSN=2297-1769

ABSTRACT=Porcine rotavirus type A (PoRVA) is the main cause of dehydrating and diarrhea in piglets, which has a great impact on the development of pig industry worldwide. A rapid, accurate and sensitive detection method is conducive to the monitoring, control and removal of PoRVA. In this study, a PoRVA real-time fluorescent reverse transcription recombinase-aided amplification (RT-RAA) assay was developed. Based on the PoRVA VP6 gene, specific primers and probes which were designed and synthesis. The sensitivity of this method was not much different than the real-time fluorescent quantitative PCR(RT-qPCR) for PoRVA, which was 7 copies per reaction. The results of RT-RAA and SYBR GreenⅠRT-qPCR methods were completely consistent in 110 clinical samples. Therefore, we successfully established a rapid and specific RT-RAA diagnostic method for PoRVA.