AUTHOR=Rutledge Jessica J. , Paegelow Jillian , Ritchey Jerry , Singh Anuradha , Rizzi Theresa , Murray Cynthia , Gilliam Lyndi , Crisman Evan , Williams Natasha J. , Holbrook Todd C. 

TITLE=Nebulized glycosylated caffeic acid phenylether ester attenuation of environmental particulate-induced airway inflammation in horses

JOURNAL=Frontiers in Veterinary Science

VOLUME=Volume 9 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2022.958567

DOI=10.3389/fvets.2022.958567

ISSN=2297-1769

ABSTRACT=The objective of this study was to determine the extent that nebulized glycosylated caffeic acid phenethyl ester-4-O-alpha-D-glucopyranoside (G-CAPE) attenuates particulate-induced airway inflammation in healthy horses. 5 healthy adult horses were housed inside a climate controlled, closed barn on straw bedding and fed ad lib moldy grass hay to induce airway inflammation. An experimental crossover study was performed in which animals were treated with 200mg G-CAPE dissolved in 45mLs of 10% triethanolamine (G-CAPE Group) or 45mL of 10% triethanolamine (CONTROL group), and clinical respiratory scoring, arterial blood gases, and bronchoalveolar lavages were collected for 24 hours. There was a significant reduction (p=0.0404) in the mean neutrophil percentage of treated horses (9.3 + 2.0) compared to controls (16.9 + 2.4) at six hours post treatment (t=6hrs) and a significant increase (p=0.0217) in the mean neutrophil percentage of control horses from t=0 to t=6hrs, from 10.3+1.6 to 16.9+2.4 (t=6hrs). Both treatment and control groups demonstrated a significantly lower mean lymphocyte percentage at t=24hrs when compared to t=6hrs (p=0.0374, p=0.0217 respectively). Blood gas analysis did not differ significantly between groups. There was a significant improvement in the mean IDEASS respiratory scores of G-CAPE-treated horses compared to controls at one hour post treatment (from 3.2+1.5 to 1.6+1.5, p = 0.0316). This study demonstrates that a single nebulized dose of G-CAPE significantly decreased clinical respiratory scores one-hour post administration and significantly decreased BAL neutrophil numbers 6 hours after administration in horses with particulate induced airway inflammation. This compound shows promise as an anti-inflammatory and warrants further investigation.